关键词: 金线莲, 银线莲, HPLC指纹图谱, 相似度评价, 栽培

Abstract: In this experiment, the fingerprints of common cultivated and wild Anoectochilus roxburgii including 14 batches of herbs from different sources were established by high performance liquid chromatography (HPLC) with gradient elution using acetonitrile-formic acid aqueous solution as the mobile phase. The results showed that the HPLC fingerprint of A. roxburgii confirmed 24 common peaks including 6 common peaks of kinsenoside and trigonelline, rhamnetin-3-O-rutinoside, quercetin, kaempferol, isorhamnetin and batatasin-III. Except for 3 batches of medicinal materials, the similarity of the other 11 batches was higher than 0.910, and the similarity was generally good. The similarity of Goodyera hachijoensis only reached 0.529, 0.721, and 0.698, all of which were lower than the similarity within 14 batches of A. roxburgi. Based on the similarity evaluation, it was concluded that differences existed between A. roxburgii and G. hachijoensis. The results provide a reference for the analysis and evaluation of the differences between A. roxburgii and G. hachijoensis.

Key words: Anoectochilus roxburgii, Goodyera hachijoensis, HPLC fingerprint, similarity evaluation, cultivation