亚热带植物科学 ›› 2022, Vol. 51 ›› Issue (3): 163-171.DOI: 10.3969/j.issn.1009-7791.2022.03.001
• 植物生理生化与分子生物学 • 下一篇
梁永鑫，吴劲松*
收稿日期:2022-04-25
接受日期:2022-05-30
出版日期:2022-06-30
发布日期:2022-10-17
通讯作者:
吴劲松
基金资助:LIANG Yong-xin, WU Jin-song*
Received:2022-04-25
Accepted:2022-05-30
Online:2022-06-30
Published:2022-10-17
Contact:
WU Jin-song
摘要： 渐狭叶烟草(Nicotiana attenuata)是烟草属植物中研究植物与昆虫、植物与病原菌互作的模式植物。本研究以八氢番茄红素脱氢酶基因(PDS)为靶标基因，建立一套以pHSE401为基因编辑载体，以潮霉素为抗性筛选标记的渐狭叶烟草高效基因编辑体系。利用该体系，获得PDS基因约80%的基因编辑效率，远远超过目前在渐狭叶烟草中报道的约30%的基因编辑效率。进一步使用WRKY70基因为靶标，对该体系对进行编辑效率验证，经测序发现WRKY70基因编辑材料中的基因编辑效率为83%，其中发生大片段缺失突变的频率为50%。因此，本研究成功建立了渐狭叶烟草高效基因编辑体系，为以后渐狭叶烟草的基因功能研究奠定基础。
中图分类号:
梁永鑫，吴劲松. 渐狭叶烟草高效基因编辑体系的建立[J]. 亚热带植物科学, 2022, 51(3): 163-171.
LIANG Yong-xin, WU Jin-song. Establishment of a High Efficient Gene Editing System for Nicotiana attenuata[J]. Subtropical Plant Science, 2022, 51(3): 163-171.
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