亚热带植物科学 ›› 2024, Vol. 53 ›› Issue (4): 363-368.DOI: 10.3969/j.issn.1009-7791.2024.04.009

• 研究论文 • 上一篇    下一篇

福建新记录种缘生穴子蕨及其叶绿体基因组分析

陈章雪1,陈 彬2,田 敏1,黄瑛琳1,张 淼1,陈炳华1*   

  1. (1. 福建师范大学生命科学学院,福建 福州 350117;2. 上海辰山植物园华东野生濒危资源植物保育中心,上海 201602)
  • 收稿日期:2024-03-25 接受日期:2024-04-07 发布日期:2024-11-20
  • 通讯作者: 陈炳华
  • 基金资助:
    福建木兰溪源省级自然保护区生物多样性研究与森林生态系统效益和经济效应评估咨询项目(HLSJ20230811)

A Newly Recorded Species of Prosaptia contigua in Fujian Province and Analysis of Its Chloroplast Genome

CHEN Zhang-xue1, CHEN Bin2, TIAN Min1, HUANG Ying-lin1, ZHANG Miao1, CHEN Bing-hua1*   

  1. (1. College of Life Sciences, Fujian Normal University, Fuzhou 350117, Fujian China; 2. East China Wild Endangered Resource Plant Conservation Center, Shanghai Chenshan Botanical Garden, Shanghai 201602, China)
  • Received:2024-03-25 Accepted:2024-04-07 Published:2024-11-20
  • Contact: CHEN Bing-hua

摘要: 报道福建省蕨类植物一新记录属—穴子蕨属Prosaptia C. Presl,及新记录种—缘生穴子蕨Prosaptia contigua (G. Forst.) C. Presl.。文中提供了相应的生境与特写图片,凭证标本保存于福建师范大学标本馆(FNU)。在形态鉴定的基础上,基于rbcL和atpB基因联合构建系统发育树,结果表明新记录种与NCBI数据库的缘生穴子蕨为同一物种(BP/PP=93/0.99),为强支持。通过二代高通量全基因组测序,组装和注释了缘生穴子蕨叶绿体基因组,其叶绿体基因组为典型的四分体结构,总长度为148 722 bp,含123个基因(31个tRNA基因、8个rRNA基因和84个蛋白质编码基因),研究结果为后续水龙骨科系统发育研究提供了数据支持。

关键词: 缘生穴子蕨;新记录;叶绿体基因组;系统发育分析

Abstract: A newly recorded genus of ferns, Prosaptia C. Presl, and a newly recorded species, Prosaptia contigua (G. Forst.) C. Presl., have been identified in Fujian Province. The corresponding habitat and detailed images are provided, and the voucher specimens are preserved in the Herbarium of Fujian Normal University (FNU). In addition to morphological identification, a phylogenetic tree was constructed based on the combined genes of rbcL and atpB. The results showed that the newly recorded species matched the P. contigua in the NCBI database (BP/PP=93/0.99), providing strong support. The chloroplast genome genome was sequenced, assembled, and annotated using the second-generation high-throughput whole genomes sequencing, revealing a typical four-stage structure with a total length of 148 722 bp and comprising123 genes (including 31 tRNA genes, 8 rRNA genes and 84 protein-coding genes). These findings provide a solid foundation for subsequent studies on the phylogeny of Polypodiaceae.

Key words: Prosaptia contigua; newly recorded species; chloroplast genome; phylogenetic analysis

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