关键词: 灵芝, 液体发酵, 培养基, 胞外多糖

Abstract: The optimum culture conditions for Ganoderma lucidum strain G18801 by liquid fermentation to yield exccellujar polysaccharide and the chemical components of these polysaccharides were studied. The suitable formula of culture fluid (g/L) was. malt sugar 40, peanut meal 30, KH₂-PO₄ 1.5, (NH₄)₂SO₄ 1.5,MgSO₄·7H₂O 0.75 and CaCO₃ 2, and the optimum initial pH was 5.5.Sixty ml culture fluid was held in 300 ml cone bottle. After shake culture (165 rpm) at 28C for 7-8 days, the yield of pure polysaccharide in fermented fluid was up to 880 mg/L. These exocellular polysaccharides could be seperated into two parts, i.e. water-soluble and water-insoluble. The monoses that made up of water soluble polysaccharide were galactose, glucose, arabinose and xylose, and the ratio was 6:4:4:1. Water-insoluble polysaccharide was glucan. The cyclic configuration of exocellular polysaccharide from Ganoderma lucidum was pyranose. As to that of the terminal configuration, galactose was a configuration and glucose was β.

Key words: Ganoderma lucidum, Liquid fermentation, Culture medium, Exocellufar polysaccharide