应用正交设计法优选黄独脱毒苗快繁培养基

doi:10.3969/j.issn.1009-7791.2009.03.006

亚热带植物科学 ›› 2009, Vol. 38 ›› Issue (03): 24-26.DOI: 10.3969/j.issn.1009-7791.2009.03.006

应用正交设计法优选黄独脱毒苗快繁培养基

尹明华,洪森荣,徐卫红,王艾平

上饶师范学院生命科学系, 江西上饶 334001

收稿日期:2009-02-11 出版日期:2009-09-10 发布日期:2009-09-10
作者简介:尹明华(1973-),女,江西吉安人,硕士,讲师,从事生物技术研究。
基金资助:
江西省教育厅科技项目(GJJ09374);上饶师范学院院级科技项目(SR0911)

Study on Rapid Propagation Medium for Virus-free Plantlets of Dioscorea bulbifera by Orthogonal Design

YIN Ming-hua, HONG Sen-rong, XU Wei-hong, WANG Ai-ping

Department of Life Science, Shangrao Normal University, Shangrao 334001, Jiangxi China

Received:2009-02-11 Online:2009-09-10 Published:2009-09-10

摘要/Abstract

摘要： 通过正交设计法研究黄独脱毒苗的快繁培养基,考察KT、NAA和2,4-D对黄独脱毒苗快繁的影响。结果表明,黄独脱毒苗快繁的最佳培养基为MS+KT 2.0 mg/L+NAA 0.5 mg/L。

关键词: 黄独, 脱毒苗, 快繁培养基, 优选

Abstract: The rapid propagation medium for virus-free plantlets of Dioscorea bulbifera were studied by orthogonal design,and the effects of rapid propagation medium including different concentrations of KT,NAA and 2,4-D on rapid propagation of Dioscorea bulbifera were also investigated.The results showed that the best rapid propagation medium for virus-free plantlets of Dioscorea bulbifera was MS+KT 2.0 mg/L+NAA 0.5 mg/L.

Key words: Dioscorea bulbifera, virus-free plantlets, rapid propagation medium, optimization

中图分类号:

Q943.1

尹明华,洪森荣,徐卫红,王艾平. 应用正交设计法优选黄独脱毒苗快繁培养基[J]. 亚热带植物科学, 2009, 38(03): 24-26.

YIN Ming-hua, HONG Sen-rong, XU Wei-hong, WANG Ai-ping. Study on Rapid Propagation Medium for Virus-free Plantlets of Dioscorea bulbifera by Orthogonal Design[J]. Subtropical Plant Science, 2009, 38(03): 24-26.

[1]	尹明华,王艾平,罗朝晖,夏瑾华,许露. KT、2,4-D和NAA对黄独组培苗带芽茎段生长发育的影响[J]. 亚热带植物科学, 2010, 39(02): 29-31.
[2]	尹明华,洪森荣,罗建民. 盐胁迫对黄独脱毒苗生长和若干生理生化指标的影响[J]. 亚热带植物科学, 2009, 38(04): 15-17.
[3]	尹明华,洪森荣. 黄独脱毒苗快繁及产业化技术探讨[J]. 亚热带植物科学, 2009, 38(02): 22-25.

应用正交设计法优选黄独脱毒苗快繁培养基

Study on Rapid Propagation Medium for Virus-free Plantlets of Dioscorea bulbifera by Orthogonal Design

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