关键词: 安祖花, 愈伤组织, 叶绿体

Abstract: Tender leaves of Anthurium andraeanum were cultured on MS medium containing optimal concentration of plant hormones, then the callus were induced and plantlets formed. Observation under electron microscope shows that there are significant differences in ultrastructure among the cells of the puff callus, the tight callus and the tender leaves of plantlets. In the cells of puff callus, cytoplasm are thin, vacuoles are bigger than normal, organelles are few, while no typical plastids and pre-plastids were observed. In the cells of tight callus and the tender leaves, cytoplasm are thick, vacuoles are smaller, the structure and the development of chloroplasts and mitochrodia can be observed clearly. It suggests that one of the causes of failing to differentiate into plantlets from longtime cultured callus is the excessive depression of chloroplasts, thus losing the function of regeneration.

Key words: Anthurium andraeanum, Callus, Chloroplast