关键词: 紫球藻, 胞外多糖, 超声波, 均匀设计, 粘度

Abstract: The effect of ultrasonic degradation of extracellular polysaccharide from Porphyridium cruentum was studied by determining the viscosity of solution. Uniform design method was used to optimize the parameter of ultrasonic, i.e. amplitude, irradiation time and pulse on the degradation of extracellular polysaccharide from Porphyridium cruentum. The optimal conditions for the viscosity were as follows:amplitude 39%, irradiation time 245s and pulse 9.5s. Under the optimal condition, the experimental value of the viscosity of the extracellular polysaccharide achieved to 2.98 mm²/s, compared to the calculated value, the error was 0%. Quadratic polynomial of DPS software was used to analyze and fit the result of the experiment, and the significance of model and regression coefficients was tested in order to establish a regression equation aimed at the viscosity of extracellular polysaccharide.

Key words: Porphyridium cruentum, extracellular polysaccharide, ultrasound, uniform design, viscosity