Box-Behnken响应面法优化超声波辅助提取辣木叶多酚工艺研究

doi:10.3969/j.issn.1009-7791.2018.02.002

亚热带植物科学 ›› 2018, Vol. 47 ›› Issue (02): 107-112.DOI: 10.3969/j.issn.1009-7791.2018.02.002

• 植物生理生化与分子生物学 • 上一篇下一篇

Box-Behnken响应面法优化超声波辅助提取辣木叶多酚工艺研究

符稳群，孙言言，李晓花，邵南津，黄贵红，苏雪燕，郑艺梅

(1.闽南师范大学生物科学与技术学院，福建漳州 363000；2.漳台休闲食品与茶饮料研究所，福建漳州 363000；3.滇西应用技术大学傣医药学院，云南景洪 666100；4.漳州市苗草王生物技术有限公司，福建漳州 363000)

收稿日期:2017-09-26 修回日期:2018-04-22 出版日期:2018-06-30 发布日期:2018-06-30
通讯作者: 符稳群
作者简介:符稳群，博士，副教授，从事食品生物技术研究。
基金资助:
福建省自然科学基金面上项目(2014J01134)；福建省教育厅科技项目(JA12212)；漳州师范学院新世纪优秀人才支持计划 (SX1106)；福建省大学生创新创业项目(201610402058)

Optimization of Ultrasound-assisted Extraction of Total Polyphenols from Moringa oleifera Leaves by Box-Behnken Response Surface Methodology

FU Wen-qun, SUN Yan-yan, LI Xiao-hua, SHAO Nan-jin, HUANG Gui-hong, SU Xue-yan, ZHENG Yi-mei

(1.Department of Biological Science & Technology, Minnan Normal University, Zhangzhou 363000, Fujian China; 2.Research Institute of Zhangzhou and Taiwan Leisure Food and Tea Beverage, Zhangzhou 363000, Fujian China; 3.School of Traditional Dai-Thai Medicine, West Yunnan University of Applied Sciences, Jinghong 666100, Yunnan China; 4.Zhangzhou Miaocaowang Biotechnology Co. Ltd., Zhangzhou 363000, Fujian China)

Received:2017-09-26 Revised:2018-04-22 Online:2018-06-30 Published:2018-06-30
Contact: FU Wen-qun

摘要/Abstract

摘要： 采用超声波辅助提取辣木Moringa oleifera叶多酚，通过单因素试验考察乙醇浓度、料液比、提取时间和提取次数对辣木叶多酚得率的影响，进一步利用四因素三水平Box-Behnken组合设计响应面试验对提取工艺参数进行优化。结果表明，超声波辅助提取辣木叶多酚的最佳工艺条件为乙醇浓度62%、料液比1∶30(W/V)、提取时间30 min、提取3次，在此条件下辣木叶多酚得率为27.32 mg·g-1，所得回归模型拟合情况良好。

关键词: 辣木, 多酚, 超声波提取, 响应面

Abstract: The optimum process of ultrasound-assisted extraction of total polyphenos from Moringa oleifera leaves by response surface methodology was studied in the research. The experiment method was designed according to Box-Behnken central composite design with 4 factors and 3 levels on the basis of single-factor experiment of ethanol concentration, material-to-liquid ration, extraction time and extraction times. The relationship between influencing factors and response values was subsequently established. The results showed that the optimum extraction conditions were as follows: the extracting solvent was 62% ethanol, material to liquid ration was 1:30 (W/V), extracting time was 30 min, and extracting three times. Under these conditions, the maximal yield of total polyphenols was upto 27.32 mg·g-1, which was well matched with the predicted content.

Key words: Moringa oleifera, polyphenol, ultrasound-assisted extraction, response surface methodology

中图分类号:

R284

符稳群,孙言言,李晓花,邵南津,黄贵红,苏雪燕,郑艺梅. Box-Behnken响应面法优化超声波辅助提取辣木叶多酚工艺研究[J]. 亚热带植物科学, 2018, 47(02): 107-112.

FU Wen-qun,SUN Yan-yan,LI Xiao-hua,SHAO Nan-jin,HUANG Gui-hong,SU Xue-yan,ZHENG Yi-mei. Optimization of Ultrasound-assisted Extraction of Total Polyphenols from Moringa oleifera Leaves by Box-Behnken Response Surface Methodology[J]. Subtropical Plant Science, 2018, 47(02): 107-112.

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Box-Behnken响应面法优化超声波辅助提取辣木叶多酚工艺研究

Optimization of Ultrasound-assisted Extraction of Total Polyphenols from Moringa oleifera Leaves by Box-Behnken Response Surface Methodology

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