关键词: 野生兰花, 种子培养, 萌发, 无菌苗

Abstract: The traits of fruits, seeds, embryos and seeds culture of four wild orchidaceous plants were studied. These plants included Calanthe discolor; Liparis japonica; Pholidota chinensis; Dendrobium densiflorum. The results showed that the colours of the seeds were white, light green or yellowish brown. The seeds were spindle or ellipsoid. The seeds of L. japonica, P. chinensis, D. densiflorum had seed pores and their embryos were dense and visible. The seeds germinated early and the germination rate was high. In which, the seeds of P. chinensis and D. densiflorum germinated earliest, and it only needed 36 days. The germination rate reached 100%. But the seeds of C. discolor did not have seed pores and the embryos could not see clearly. The seeds germinated late and the germination rate was low. The seeds of four orchidaceous plants were germinated by protocorms. A group of aseptic seedlings had been achieved through subculture. The germination status was discussed in combination with the characteristics of fruits, seeds and embryos.

Key words: wild orchidaceous plants, seed culture, germination, aseptic seedlings