火百合花丝组织培养器官形成的细胞组织学研究

亚热带植物科学 ›› 2006, Vol. 35 ›› Issue (02): 5-8.

火百合花丝组织培养器官形成的细胞组织学研究

周祖富,艾素云,杨美纯,黎兆安

广西大学农学院, 广西南宁 530005

收稿日期:2005-11-28 出版日期:2006-06-10 发布日期:2006-06-10
作者简介:周祖富(1951-),男,广西阳朔人,高级工程师,从事植物生理生化和组织培养研究。
基金资助:
广西科技厅资助项目(桂科攻0322008-2A)

Cytohistological Study on Morphogenesis in filament Culture of Lilium‘Star Gazer’

ZHOU Zu-fu, AI Su-yun, YANG Mei-chun, LI Zhao-an

Agricultural College, Guangxi University, Nanning 530005, Guangxi China

Received:2005-11-28 Online:2006-06-10 Published:2006-06-10

摘要/Abstract

摘要： 以火百合花丝节段为外植体,接种在附加6-BA 1.0 mg/L和NAA 0.2 mg/L的MS培养基上,诱导出愈伤组织及器官。经细胞组织学观察表明,细胞启动、愈伤组织形成及器官再生皆发生于外植体形态学下端的切口边缘及内方,而形态学上端的细胞自始至终未启动。器官发生的途径是通过愈伤组织间接产生,有的愈伤组织团单独形成芽或根,而有的愈伤组织团则同时分别从表面形成芽,内部形成根,通过维管组织连接成完整的植株。

关键词: 火百合, 花丝, 器官发生, 细胞组织学

Abstract: Segments from filament of Lilium ‘star gazer’ were cultured as explants on MS medium supplemented with BA 1.0 mg/L and NAA 0.2 mg/L to induce callus and organs.Cytohistological observations on callus and bud formation were carried out.The results were as follows.Callus was initiated from the cut surface of the morphological lower side of explants;Some callus packs developed into roots or shoots,and others developed into shoots from surface cells and developed into roots from inner cells.

Key words: Lilium ‘star gazer’, filament, organogenesis, cytohistology

中图分类号:

Q944.6

周祖富,艾素云,杨美纯,黎兆安. 火百合花丝组织培养器官形成的细胞组织学研究[J]. 亚热带植物科学, 2006, 35(02): 5-8.

ZHOU Zu-fu, AI Su-yun, YANG Mei-chun, LI Zhao-an. Cytohistological Study on Morphogenesis in filament Culture of Lilium‘Star Gazer’[J]. Subtropical Plant Science, 2006, 35(02): 5-8.

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