亚热带植物科学 ›› 2004, Vol. 33 ›› Issue (02): 44-46.

• 研究报告 • 上一篇    下一篇

蚊净香草快速繁殖研究

卢秉国,曹智,陈昭   

  1. 福建师范大学 福清分校 生化系, 福建 福清 350300
  • 收稿日期:2004-04-02 出版日期:2004-06-10 发布日期:2004-06-10
  • 作者简介:卢秉国(1974-),男,福建仙游人,硕士,从事植物生物技术研究。

Rapid propagation of Mozzie buster

LU Bing-guo, CAO Zhi, CHEN Zhao   

  1. Biological and Chemical Engineering Department of Fuqing Branch of Fujian Normal University, Fuqing 350300, Fujian China
  • Received:2004-04-02 Online:2004-06-10 Published:2004-06-10

摘要: 以蚊净香草嫩叶和叶柄为外植体进行培养,筛选合适的外植体与诱导、增殖和生根的最佳培养基。结果表明,叶片外植体在MS+6-BA 2.0mg/L+NAA 0.5mg/L培养基最适于诱导愈伤组织和不定芽;MS+6-BA 1.0mg/L+NAA 0.3mg/L培养基有利于不定芽增殖;无根苗在含有低浓度无机盐和生长素水平的生根培养基上易生根,生根率高达100%。

关键词: 蚊净香草, 组织培养, 快速繁殖

Abstract: Taking the petioles and leaves of Mozzie buster as explants, tissue culture and rapid propagation were studied. The results showed that the leaves cultured on MS media supplemented with 6-BA 2.0mg/L and NAA 0.5mg/L was best for inducing callus and adventitious buds, and MS media with 6-BA 1.0mg/L and NAA 0.3mg/L was suitable for subculture of adventitious buds. The microshoots rooted in low salt media containing low level of auxin, rooting rate up to 100%.

Key words: Mozzie buster, tissue culture, rapid propagation

中图分类号: