关键词: 雷公藤, 组织培养, 体系建立

Abstract: Using the plant without diseases and insect pests as experimental material, the renewable technologies of stem section, leaf in Tripterygium wilfordii were studied by orthogonal experimental design. The results showed that the optimal medium for bud induction was MS + 6-BA 1.5 mg·L-1 + IBA 0.1 mg·L-1; the optimal medium for subculture was MS + 6-BA 1.0 mg·L-1 + NAA 0.1 mg·L-1; the best culture medium for seedling cultivation was MS + 6-BA 0.5 mg·L-1 + VB1 0.5 mg·L-1 + Peptone 0.5 g·L-1; the optimal rooting medium was 1/2MS + NAA 2.0 mg·L-1 + KT 0.1 mg·L-1. In transplanting, the best substrate ratio was vermiculite and peat mixed with 1:1, and 30 days after transplanted, the transplanting survival rate was up to 84.5%.

Key words: Tripterygium wilfordii, tissue culture, system establishment