蓝光胁迫对金线莲游离氨基酸组分的影响

doi:10.3969/j.issn.1009-7791.2021.06.003

亚热带植物科学 ›› 2021, Vol. 50 ›› Issue (06): 439-446.DOI: 10.3969/j.issn.1009-7791.2021.06.003

蓝光胁迫对金线莲游离氨基酸组分的影响

王弛1，刘慧1，吴文晞2，褚克丹1，赵峰1*

(1. 福建中医药大学药学院，福建福州 350122；2. 福建省恒正检测技术有限公司，福建福州 350199)

出版日期:2021-12-30 发布日期:2022-03-31
通讯作者: 福建中医药大学引进人才科研启动项目(2801/701190097)

Effect of Blue Light Stress on Free Amino Acids of Anoectochilus roxburghii

WANG Chi 1, LIU Hui 1, WU Wen-xi 2, CHU Ke-dan 1, ZHAO Feng 1*

(1. College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian China; 2. Fujian Hengzheng Testing Company, Fuzhou 350199, Fujian China)

Online:2021-12-30 Published:2022-03-31

摘要/Abstract

摘要： 以金线莲(Anoectochilus roxburghii)为试材，在光密度30 μmol·m–2s–1的白光基础上补充60 μmol·m–2·s–1、120 μmol·m–2·s–1和180 μmol·m–2·s–1三个强度蓝光胁迫56 d，采用氨基喹啉-N-羟基丁二酰氨基甲酸酯(AQC)柱前衍生结合超高效液相色谱-串联质谱法测定游离氨基酸组分含量变化，分析金线莲游离氨基酸组分对蓝光胁迫的响应特征。共稳定检测出22种氨基酸，苏氨酸、丙氨酸、谷氨酰胺、缬氨酸、肌氨酸、组氨酸、天冬酰胺和酪氨酸是金线莲的特征氨基酸，游离氨基酸总量随着胁迫时间延长呈先增加后减少的趋势，在14 d 时含量最高。综合结果表明，蓝光胁迫14?d对金线莲中多种游离氨基酸的合成有促进作用，在补充180 μmol·m–2·s–1强度蓝光时效果最好。

关键词: 金线莲, 蓝光胁迫, 游离氨基酸, AQC衍生

Abstract: Anoectochilus roxburghii was used as the test material, and the white light with an optical density of 30 μmol·m–2·s–1 was supplemented with three intensities of 60 μmol·m–2·s–1, 120 μmol·m–2·s–1 and 180 μmol·m–2·s–1 blue light stress for 56 days. Aminoquinoline-N-hydroxysuccinyl carbamate (AQC) pre-column derivatization combined with ultra performance liquid chromatography-tandem mass spectrometry was used to determine the content of free amino acids in order to clarify the response characteristics of the free amino acid components for blue stress. A total of 22 amino acids were detected stably. Threonine, Alanine, Glutamine, Valine, Sarcosine, Histidine, Asparagine and Tyrosine were the characteristic amino acids in A. roxburghii. The total amount of free amino acids increased first and then decreased with the extension of the stress time, and the content was the highest on the 14th day. The comprehensive results showed that blue light stress for 14 days could promote the synthesis of a variety of free amino acids in A. roxburghii, and the effect was best when supplemented with 180 μmol·m–2·s–1 intensity of blue light.

Key words: Anoectochilus roxburghii, blue light stress, free amino acid, AQC derivative

中图分类号:

Q945

王弛, 刘慧, 吴文晞, 褚克丹, 赵峰. 蓝光胁迫对金线莲游离氨基酸组分的影响[J]. 亚热带植物科学, 2021, 50(06): 439-446.

WANG Chi, LIU Hui, WU Wen-xi, CHU Ke-dan, ZHAO Feng. Effect of Blue Light Stress on Free Amino Acids of Anoectochilus roxburghii[J]. Subtropical Plant Science, 2021, 50(06): 439-446.

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蓝光胁迫对金线莲游离氨基酸组分的影响

Effect of Blue Light Stress on Free Amino Acids of Anoectochilus roxburghii

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