›› 2015, Vol. 44 ›› Issue (01): 23-28.DOI: 10.3969/j.issn.1009-7791.2015.01.005

• 植物生理生化与分子生物学 • 上一篇    下一篇

也门铁的组织培养技术研究

齐安民,王家琼,李楠,贺涛,李薇,崔大方   

  1. (1.华南农业大学 林学院,广东 广州 510642;2.重庆云阳县果品产业发展局,重庆 云阳 404500;3.重庆奉节县林业局,重庆 奉节 404600;4.华南农业大学 公共基础课实验教学中心,广东 广州510642;5.广州南沙明珠湾区开发有限公司,广东 广州 511455)
  • 收稿日期:2014-11-26 修回日期:2015-04-18 出版日期:2015-03-10 发布日期:2015-03-10
  • 通讯作者: 崔大方
  • 作者简介:齐安民,硕士,从事生物技术与园艺植物资源研究。

Tissue Culture Technique of Dracaena fragrans

QI An-min,WANG Jia-qiong,LI Nan,HE Tao,LI Wei,CUI Da-fang   

  1. (1.College of Forestry, South China Agricultural University, Guangzhou 510642, Guangdong China; 2.Yunyang Fruit Industry Development Council, Yunyang 404500, Chongqing China; 3.Fengjie Forestry Bureau, Fengjie 404600, Chongqing China; 4. Center of Experimental Teaching for common Basic Courses, South China Agricultural University, Guangzhou 510642, Guangdong China; 5.Nansha Pearl Bay Development Co. Ltd., Guangzhou 511455, Guangdong China )
  • Received:2014-11-26 Revised:2015-04-18 Online:2015-03-10 Published:2015-03-10
  • Contact: CUI Da-fang

摘要: 以4品种也门铁的茎段为外植体进行组织培养技术研究。结果表明,控制普通也门铁茎段褐化效果最理想的培养基为MS + 0.25 g·L-1 VC + 0.50 g·L-1 Na2S2O3;诱导也门铁不定芽萌动的最佳培养基为MS + 3.0 mg·L-1 6-BA + 0.2 mg·L-1 NAA + 0.1 mg·L-1 KT;诱导也门铁不定芽增殖的最佳培养基,因品种不同而异,普通也门铁和金心也门铁为MS + 2.0 mg·L-1 6-BA + 1.0 mg·L-1 NAA + 0.1 mg·L-1 GA3,扭纹铁和金心扭纹铁为MS + 1.0 mg·L-1 6-BA + 0.5 mg·L-1 NAA + 0.1 mg·L-1 GA3;也门铁壮苗的最佳培养基为MS + 0.05 mg·L-1 6-BA + 0.05 mg·L-1 NAA + l g·L-1AC;也门铁生根最优培养基为1/2MS + 0.5 mg·L-1 IBA。

关键词: 也门铁, 组织培养, 褐化

Abstract: The tissue culture techniques of four cultivars of Dracaena fragrans used stem section as the explant were analyzed. A series of important conclusions can be made: 1) The optimal medium for controlling tissue browning of D. fragrans: MS + 0.25 g·L-1 VC + 0.50 g·L-1 Na2S2O3; 2) The optimal medium for inducing the D. fragrans stem stirring: MS+ 3.0 mg·L-1 6-BA + 0.2 mg·L-1 NAA + 0.1 mg·L-1 KT; 3) The optimal proliferation medium of D. fragrans, depends on the cultivars of D. fragrans. The optimal medium for D. fragrans and D. fragrans ‘Massangeana’: MS + 2.0 m g·L-1 6-BA + 1.0 mg·L-1 NAA + 0.1 mg·L-1 GA3; The optimal medium for D. fragrans ‘Compacta’ and D. fragrans ‘Janet-craig’: MS + 1.0 m g·L-1 6-BA + 0.5 mg·L-1 NAA + 0.1 mg·L-1 GA3; 4) Under the condition of MS + 0.05 mg·L-1 6-BA + 0.05 mg·L-1 NAA + l g·L-1 AC was the most efficient the Dracaena fragrans seedling medium; 5) 1/2MS + 0.5 mg·L-1 IBA was the most efficient rooting medium.

Key words: Dracaena fragrans, tissue culture, callus browning

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