关键词: 灵芝, 液体发酵, 菌龄, 培养基, 多糖

Abstract: Some factors influencing liquid fermentation of Ganoderma lucidum were examined. The experiments showed that 4-day old spawn and 60 ml of culture medium in 300 ml shake-bottle gave the best fermentation result. Sucrose and peanut meal were ideal carbon source and nitrogen source, respectively. The optimum formula of culture medium selected was. sucrose 4%, peanut meal 3%, (NH₄)₂SO₄ 0.15%, KH₂PO₄ 0.15%. The dry weight of mycelium and polysaccharide were up to 0.83 g and 0.3 g per 100 ml fermented liquid, respectively. These results provide the basis for increasing yield and active constituents of fermented pro-duct of Ganoderma lucidum.

Key words: Ganoderma lucidunt, liquid fermentation, age of spawn, cul-ture medium, polysaccharide