Abstract: The quality marker of Peucedani Radix was identified for quality control in this study. The HPLC method was employed to establish a quantitative method for coumarins in multiple batches of Peucedanum praeruptorum. The content changes of five major coumarins in the decoction pieces and decoctions of P. praeruptorum were analyzed. Finally, the quality markers of P. praeruptorum were screened through network pharmacology and molecular docking. Results showed that the content determination of praeruptorin A, praeruptorin B, peucedanin E, imperatorin, and bergapten showed a high degree of stability. Moreover, these five components exhibited good transferability and a relatively high transfer rate under different extraction methods from decoction pieces to decoctions. The results of network pharmacology inferred that praeruptorin A, imperatorin, and peucedanin E regulated signaling pathways such as IL-17, NF-κB, C-type lectin receptor, and influenza A by binding to targets like RELA, TNF, and PTGS2, and played a role in treating diseases such as coronary heart disease, tumors, cough, and asthma. Praeruptorin A, praeruptorin B, and praeruptorin E are proposed as quality marker for P. praeruptorum.

Key words: Peucedani Radix, high performance liquid chromatography, assay of multi-component, network pharmacology, quality marker

摘要： 采用HPLC建立多批次前胡香豆素的定量方法，分析前胡5种主要香豆素在饮片-汤剂中的含量变化，最终通过网络药理学与分子对接筛选前胡质量标志物，为前胡质量评价提供依据。结果表明，白花前胡甲素、白花前胡乙素、白花前胡素E、欧前胡素及佛手柑内酯的含量检测表现出高度稳定性，而且这5种成分在饮片-汤剂的不同提取方法下，均具有良好的传递性，且转移率较好。网络药理学推测白花前胡甲素、欧前胡素、白花前胡素E通过与RELA、TNF、PTGS2等靶标相结合，调控IL-17、NF-kappa B、C型凝集素受体、甲型流感等信号通路，发挥治疗冠心病、肿瘤及咳喘等疾病的作用。研究结果表明白花前胡甲素、白花前胡素E以及欧前胡素可作为前胡质量标志物。

关键词: 前胡, 高效液相色谱, 多成分定量, 网络药理学, 质量标志物