Abstract: The contents of endogenous IAA and ABA in the differentiation of adventitious root in vitro of Elaeocarpus sylvestris were studied.The results showed that the contents of endogenous IAA and ABA in plantlets in vitro treated with IBA and NAA increased before rooting, and then began to decrease when the root was observed;the ratios of IAA to ABA were 2.526 and 3.226, respectively when the calli were visible.Treated without any hormones, the content of endogenous IAA maintained low level, but the content of endogenous ABA increased constantly in root differentiation.Accordingly the ratio of IAA to ABA was under 1.211.

Key words: Elaeocarpus sylvestris, endogenous, IAA, ABA, differentiation of adventitious root

摘要： 本文研究山杜英组培苗生根过程中内源IAA、ABA含量变化规律。结果表明,培养基添加IBA和NAA后,在生根过程中内源IAA、ABA含量变化类似,根点出现前内源IAA、ABA含量一直上升,根点出现后含量开始下降,产生愈伤组织时两种处理的IAA/ABA分别是2.526和3.226。在不添加外源生长素情况下,内源IAA含量一直维持在较低水平,而内源ABA含量一直呈现上升趋势,IAA/ABA始终都在1.211以下。

关键词: 山杜英, 内源, IAA, ABA, 根分化