Making Template DNA in the Analysis of Tobacco Germplasm Based on AFLP Fingerprint

Subtropical Plant Science ›› 2005, Vol. 34 ›› Issue (02): 1-4.

Making Template DNA in the Analysis of Tobacco Germplasm Based on AFLP Fingerprint

YANG You-cai¹, ZHOU Qing-ming¹, YIN Han-qi²

1. College of Agronomy, Hunan Agricultural University, Changsha 410128, Hunan China;
2. College of Life Science, Zhongshan University, Guangzhou 510275, Guangdong China

Received:2004-11-02 Online:2005-06-10 Published:2005-06-10

烟草种质资源AFLP分析中DNA模板的制备

杨友才¹,周清明¹,尹晗琪²

1. 湖南农业大学农学院, 湖南长沙 410128;
2. 中山大学生命科学院, 广东广州 510275

作者简介:杨友才(1969-),男,湖南湘阴人,副教授,博士研究生,从事作物育种研究。
基金资助:
湖南省科技厅重大科技攻关项目(01NKY1002);湖南省烟草公司资助项目

Abstract

Abstract: Two methods-CTAB method and SDS method were tested in isolation of tobacco genomic DNA. The results showed that the degree of purity of DNA extracted by using CTAB method was higher than that by using SDS method; the value of OD₂₆₀/OD₂₈₀ for the DNA samples obtained with CTAB method was over 1.8; the molecular weight of DNAs was more than 23kb, the DNAs were completely digestible with EcoRⅠ/MseⅠ, and the AFLP figure were obtained.

Key words: tobacco, AFLP, DNA extraction

摘要： 采用CTAB法和SDS法提取烟草基因组DNA,经检测表明,CTAB法提取的基因组DNA纯度高于SDS法;CTAB法提取的DNA,其OD₂₆₀/OD₂₈₀值均高于1.8,相对分子量23kb左右,且能完全被EcoRⅠ和MseⅠ酶切消化,并能获得清晰的AFLP指纹图谱。

关键词: 烟草, AFLP, DNA提取方法

CLC Number:

Q781

YANG You-cai, ZHOU Qing-ming, YIN Han-qi. Making Template DNA in the Analysis of Tobacco Germplasm Based on AFLP Fingerprint[J]. Subtropical Plant Science, 2005, 34(02): 1-4.

杨友才,周清明,尹晗琪. 烟草种质资源AFLP分析中DNA模板的制备[J]. 亚热带植物科学, 2005, 34(02): 1-4.

References

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