Subtropical Plant Science ›› 2005, Vol. 34 ›› Issue (04): 1-5.

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Induction and Conservation of Callus from Loquat Leaves

YANG Feng-Ling1,2, LIN Shun-Quan1   

  1. 1. College of Horticulture, South China Agricultural University, Guangzhou 510642, Guangdong China;
    2. Linyi Teacher's College, Linyi 276005, Shandong China
  • Received:2005-07-04 Online:2005-11-10 Published:2005-11-10

枇杷叶片愈伤组织的诱导与保存

杨凤玲1,2,林顺权1   

  1. 1. 华南农业大学 园艺学院, 广东 广州 510642;
    2. 临沂师范学院, 山东 临沂 276005
  • 作者简介:杨凤玲(1980-),女,山东临沂人,助教,硕士,从事植物生物技术研究与教学。

Abstract: Loquat leaves of medium maturity was optimum for callus induction. MS+6-BA 1.0mg/L+2,4-D 0.5mg/L induced the callus with optimal quality and MS+6-BA 0.5~1.0mg/L+2,4-D 0.1~0.2mg/L gave the best result for subculture of loquat.

Key words: loquat, leaf, tissue culture

摘要: 在不同成熟度的枇杷叶片中,最适宜诱导愈伤组织的是中度成熟叶;最适合的愈伤组织诱导培养基是MS+6-BA 1.0mg/L+2,4-D 0.5mg/L,诱导出的愈伤组织为黄绿色、颗粒状、硬度适中;适合愈伤组织继代保存的培养基为MS+6-BA 0.5~1.0mg/L+2,4-D 0.1~0.2mg/L。

关键词: 枇杷, 叶片, 组织培养

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