Subtropical Plant Science ›› 2013, Vol. 42 ›› Issue (03): 193-198.DOI: 10.3969/j.issn.1009-7791.2013.03.002

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Optimization of Two-Dimensional Gel Electrophoresis System for Analyzing ‘Hongroumiyou’ Juice Sacs Proteins

WU Yang,CHEN Qing-xi   

  1. (College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian China)
  • Received:2013-04-24 Revised:2013-04-24 Online:2013-09-01 Published:2013-09-01
  • Contact: CHEN Qing-xi

红肉蜜柚汁胞蛋白质双向电泳体系优化研究

伍洋,陈清西   

  1. (福建农林大学 园艺学院,福建 福州,350002)
  • 通讯作者: 陈清西
  • 作者简介:伍洋(1987-),男,福建福州人,硕士研究生,从事果树生物技术研究。E-mail: 381794641@qq.com
  • 基金资助:

    国家科技支撑计划项目(2007BAD07B01)

Abstract: In order to establish proteomics research system for analyzing ‘Hongroumiyou’ juice sacs proteins, using ‘Hongroumiyou’ juice sacs in initial color change stage as test material, exploring the most suitable 2-DE system parameters. The results indicated that compared with TCA-acetone extraction method, phenol extraction method was more appropriate for ‘Hongroumiyou’ juice sacs total protein yield. Using 18 cm, pH 4~7 linner immobiline combined with 18 cm, pH 3~10 linner immobiline could had clear and high resolution 2-DE patterns. Both of the best isoelectric focused parameter was 43 000 vhs and 30 000 vhs, respectively.

Key words: Citrus grandis ‘Hongroumiyou’, juice sacs, proteomics, two-dimensional electrophoresis

摘要: 为建立适合红肉蜜柚发育过程中汁胞蛋白质组学的研究体系,以着色初始时期的红肉蜜柚汁胞为试材,摸索最适合的蛋白质双向电泳体系参数。结果表明,酚提取法比TCA-丙酮法更适合于红肉蜜柚汁胞总蛋白质的提取,提取得率高,双向电泳图谱清晰。采取18 cm、线性pH 3~10 和18 cm、线性pH 4~7 相结合的分析策略,可获得更为完整的蛋白质组信息,其第一向等电聚焦最佳参数(总伏小时数)分别为30 000 Vhs和43 000 Vhs。

关键词: 红肉蜜柚, 汁胞, 蛋白质组学, 双向电泳

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