Comparison of Physiological and Biochemical Properties of Storage Roots Between High and Low Starch Cultivars of Cassava during the Critical Growth Period

doi:10.3969/j.issn.1009-7791.2023.02.001

Subtropical Plant Science ›› 2023, Vol. 52 ›› Issue (2): 85-92.DOI: 10.3969/j.issn.1009-7791.2023.02.001

• Plant physiology and biochemistry • Previous Articles Next Articles

Comparison of Physiological and Biochemical Properties of Storage Roots Between High and Low Starch Cultivars of Cassava during the Critical Growth Period

CAI Zheng, SUN Jin-liang, LIU Kun-hang, LI You-zhi, FAN Xian-wei^*

(College of Life Science and Technology, Guangxi University State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Nanning 530004, Guangxi China)

Received:2023-01-07 Accepted:2023-01-28 Online:2023-04-30 Published:2023-05-15
Contact: FAN Xian-wei

木薯高、低粉品种贮藏根生长关键期的生理生化特性比较

蔡政，孙津梁，刘坤行，李有志，樊宪伟^*

(广西大学生命科学与技术学院／亚热带农业生物资源保护与利用国家重点实验室，广西南宁 530004)

通讯作者: 樊宪伟
基金资助:
国家自然科学基金项目地区基金(3216150111)；广西自然科学基金重点项目(2021GXNSFDA196009)

Abstract

Abstract: This study explored the differences and dynamic changes in the main physiological and biochemical characteristics of roots in high and low starch cultivars of cassava (Manihot esculenta) at key developmental stages (storage root formation and bulking), providing a theoretical basis for cassava management and breeding improvement. Storage root samples of high starch cassava varieties ‘FX01’ and ‘KU50’ and low starch cassava varieties ‘SC124’ and ‘9I’ were selected at 120, 130, 140, 151 and 165 days after planting, respectively. The physiological and biochemical indexes of starch, soluble sugar, abscisic acid (ABA), protective enzyme and ROS were measured and analyzed. The results showed that the soluble sugar content in the cortex of cassava was always higher than that in starch storage area. The soluble sugar content of the high starch varieties increased rapidly during the root expansion period of storage, and showed higher antioxidant enzyme activity and lower ROS and MDA content. ABA content of high starch varieties was lower than that of low starch varieties. Analyzing the effects of these physiological and biochemical parameters on starch accumulation, it was found that the distribution of soluble sugar in low starch and high starch varieties was consistent with the law of starch synthesis after transportation. The starch accumulation rate in high starch varieties was always higher than that in low starch varieties. Their higher antioxidant enzyme activities and lower ROS and MDA content were conducive to the starch accumulation in cassava storage roots.

Key words: Manihot esculenta, starch, soluble sugar, ABA, MDA, ROS

摘要： 通过研究高粉和低粉木薯Manihot esculenta品种在贮藏根发育关键期(形成-膨大期)主要生理生化性质的差异及其动态变化，为木薯栽培管理和育种改良提供理论基础。对高粉木薯品种‘辐选01’(FX01)和‘Kasetsart 50’ (KU50)以及低粉品种‘华南124’ (SC124)和‘9I’于种植后120、130、140、151和165 d五个时期分别取贮藏根样品，测定淀粉、可溶性糖、脱落酸(ABA)、保护酶、活性氧(ROS)等生理生化指标并分析变化规律。结果表明，可溶性糖含量在木薯皮层中始终高于淀粉储藏区；高粉品种中总可溶性糖含量在贮藏根膨大期快速增加，且表现出较高的抗氧化酶活性和较低的ROS及MDA含量；ABA含量呈现出高粉品种比低粉品种低的趋势。木薯低粉和高粉品种中可溶性糖的分布与其转运后合成淀粉的规律相一致，高粉品种中淀粉的积累速率始终高于低粉品种，其较高的抗氧化酶活性和较低的ROS及MDA含量有利于木薯贮藏根中淀粉的积累。

关键词: 木薯, 淀粉, 可溶性糖, ABA, MDA, ROS

CLC Number:

S533

CAI Zheng, SUN Jin-liang, LIU Kun-hang, LI You-zhi, FAN Xian-wei. Comparison of Physiological and Biochemical Properties of Storage Roots Between High and Low Starch Cultivars of Cassava during the Critical Growth Period[J]. Subtropical Plant Science, 2023, 52(2): 85-92.

蔡政, 孙津梁, 刘坤行, 李有志, 樊宪伟. 木薯高、低粉品种贮藏根生长关键期的生理生化特性比较[J]. 亚热带植物科学, 2023, 52(2): 85-92.

References

[1] Zidenga T, Leyva–Guerrero E, Moon H, Siritunga D, Sayre R. Extending cassava root shelf life via reduction of reactive oxygen species production [J]. Plant Physiology, 2012, 159(4): 1396–1407.
[2] Dai D, Hu Z, Pu G, Li H, Wang C. Energy efficiency and potentials of cassava fuel ethanol in Guangxi region of China [J]. Energy Conversion and Management, 2006, 47(13): 1686–1699.
[3] Cock J H, Connor D J. Cassava [M]. Elsevier: Crop Physiology Case Histories for Major Crops, 2021: 588–633.
[4] 赵大伟, 宋记明, 刘炜林, 孟凡来, 邓国军, 黄兴粉. 不同生态环境木薯高产高效栽培分析[J]. 中国农学通报, 2021, 37(28): 14–19.
[5] 肖明昆, 刘光华, 宋记明, 刘倩, 段春芳, 姜太玲, 张林辉, 严炜, 沈绍斌, 周迎春, 熊贤坤, 罗鑫, 白丽娜, 李月仙. 不同木薯品种(系)农艺性状分析及高产品种(系)筛选[J]. 作物杂志, 2022(4): 77–82.
[6] 靳亚忠, 任金立, 齐娟, 兰慧, 李宗佑, 吴兴彪, 张天一, 王雪娇, 于佳, 何化强, 张鹏. 薄皮甜瓜品系糖、酸、淀粉含量差异性研究 [J]. 四川农业大学学报, 2020, 38(6): 723–733.
[7] 孙继, 顾万荣, 赵东旭, 孟繁美, 魏湜. 不同株型玉米灌浆期穗位叶可溶性糖含量和子粒淀粉积累关系的研究[J]. 作物杂志, 2012(2): 80–83.
[8] 张秋英, 刘娜, 金剑, 刘晓冰, 杨恕平, 王光华. 春小麦籽粒淀粉和蛋白质积累与底物供应的关系[J]. 麦类作物学报, 2000(1): 55–58.
[9] 沈淞海, 沈海铭, 吴建华. 甘薯生长发育过程中可溶性糖含量与淀粉积累的关系[J]. 浙江农业大学学报(农业与生命科学版), 1994(4): 400–404.
[10] Li M, Yang S, Xu W, Pu Z, Feng J, Wang Z, Zhang C, Peng M, Du C, Lin F, Wei C, Qiao S, Zou H, Zhang L, Li Y, Yang H, Liao A, Song W, Zhang Z, Li J, Wang K, Zhang Y, Lin H, Zhang J, Tan W. The wild sweet potato (Ipomoea trifida) genome provides insights into storage root development [J]. BMC Plant Biology, 2019, 19: 1–17.
[11] Mehdi R, Lamm C E, Anjanappa R B, Mudsam C, Saeed M, Klima J, Kraner M E, Ludewig F, Knoblauch M, Gruissem W, Sonnewald U, Zierer W. Symplasmic phloem unloading and radial post-phloem transport via vascular rays in tuberous roots of Manihot esculenta [J]. Journal of Experimental Botany, 2019, 70(20): 5559–5573.
[12] Pan K, Lu C, Nie P, Hu M Z, Zhou X C, Chen X, Wang W Q. Predominantly symplastic phloem unloading of photosynthates maintains efficient starch accumulation in the cassava storage roots (Manihot esculenta Crantz) [J]. BMC Plant Biology, 2021, 21(1): 318.
[13] 罗兴录, 潘晓璐, 朱艳梅. 木薯内源ABA含量与块根淀粉积累关系研究[J]. 热带作物学报, 2018, 39(3): 472–479.
[14] 彭佳铭, 屈仁军, 王世威, 王新新, 查良平, 彭华胜, 申业. 丹参超氧化物歧化酶SmMSD2基因的克隆与表达分析[J]. 药学学报, 2023, 58(2): 454–464.
[15] 田国忠, 李怀方, 裘维蕃. 植物过氧化物酶研究进展[J]. 武汉植物学研究, 2001(4): 332–344.
[16] 欧阳翠. 高低淀粉木薯品种块根可溶性糖含量与块根淀粉积累研究[J]. 农业科技通讯, 2018(1): 78–82.
[17] Cheng Y E, Dong M Y, Fan X W, Nong L L, Li Y Z. A study on cassava tolerance to and growth responses under salt stress [J]. Environmental and Experimental Botany, 2018, 155: 429–440.
[18] Hosseini S A, Maillard A, Hajirezaei M R, Ali N, Schwarzenberg A, Jamois F, Yvin J C. Induction of barley silicon transporter HvLsi1 and HvLsi2, increased silicon concentration in the shoot and regulated starch and ABA homeostasis under osmotic stress and concomitant potassium deficiency [J]. Frontiers in Plant Science, 2017, 8: 1359–1373.
[19] 刘玉明, 钱甜甜, 蒋定文, 何颖, 沈先荣, 江叔奇. 凯氏定氮法和考马斯亮蓝法测定方格星虫多糖中蛋白质的含量[J]. 中国实验方剂学杂志, 2013, 19(19): 96–98.
[20] 张秀梅, 杜丽清, 谢江辉, 陈佳瑛, 弓德强, 李伟才. 高效液相色谱法测定菠萝果实中的糖分[J]. 食品科学, 2007(11): 450–452.
[21] Velikova V, Yoranov I, Edreva A. Oxidative stress and some antioxidant systems in acid rain-treated bean plants-Protective role of exogenous polyamines [J]. Plant Science, 2000, 151(1): 59–66.
[22] Sinnhuber R O, Yu T C, Chang Y T. Characterization of the red pigment formed in the 2-thiobarbituric acid determination of oxidative rancicity a,b [J]. Journal of Food Science, 2010, 23(6): 626–634.
[23] 秦延河, 罗丹明. B-Mn2+-H2O2体系光度法测定水果的抗氧化性[J]. 江西师范大学学报(自然科学版), 2005(6): 519–521.
[24] 王爱国, 罗广华. 植物的超氧物自由基与羟胺反应的定量关系[J]. 植物生理学通讯, 1990(6): 55–57.
[25] 李合升. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000.
[26] Lin C C, Kao C H. NaCl induced changes in ionically bound peroxidase activity in roots of rice seedlings [J]. Plant and Soil, 1999, 216(1–2): 147–153.
[27] Misako K, Seki Shimiz. Chlorophyll metabolism in higher plants VI. Involvement of peroxidase in chlorophyll degradation [J]. Plant Cell Physiology, 1985, 26(7):?1291–1301.
[28] Kumar R, Mukherjee S, Ayele B T. Molecular aspects of sucrose transport and its metabolism to starch during seed development in wheat: A comprehensive review [J]. Biotechnology Advances, 2018, 36(4): 954–967.
[29] Fu Y Y, Gu Q Q, Dong Q, Zhang Z H, Lin C, Hu W M, Pan R H, Guan Y J, Hu J. Spermidine enhances heat tolerance of rice seeds by modulating endogenous starch and polyamine metabolism [J]. Molecules, 2019, 24(7): 1395.
[30] Baxter A, Mittler R, Suzuki N. ROS as key players in plant stress signalling [J]. Journal of Experimental Botany, 2014, 65(5): 1229–1240.
[31] Inzé A, Vanderauwera S, Hoeberichts F A, Vandorpe M, Van Gaever T, Van Breusegem F. A subcellular localization compendium of hydrogen peroxide-induced proteins [J]. Plant Cell and Environment, 2012, 35(2): 308–320.
[32] Mittler R, Vanderauwera S, Suzuki N, Miller G, Tognetti V B, Vandepoele K, Gollery M, Shulaev V, Van Breusegem F. ROS signaling: the new wave? [J]. Trends in Plant Science, 2011, 16(6): 300–309.
[33] Ortega-Galisteo A P, Rodriguez-Serrano M, Pazmino D M, Gupta D K, Sandalio L M, Romero-Puertas M C. S-Nitrosylated proteins in pea (Pisum sativum L.) leaf peroxisomes: changes under abiotic stress [J]. Journal of Experimental Botany, 2012, 63(5): 2089–2103.
[34] Eliyahu E, Rog I, Inbal D, Danon A. ACHT4-driven oxidation of APS1 attenuates starch synthesis under low light intensity in Arabidopsis plants [J]. Proceedings of the national academy of sciences of The United States of America, 2015, 112(41): 12876–12881.
[35] Del Rio L A. ROS and RNS in plant physiology: an overview [J]. Journal of Experimental Botany, 2015, 66(10): 2827–2837.
[36] Xu N, Chu Y L, Chen H L, Li X X, Wu Q, Jin L, Wang G X, Huang J L. Rice transcription factor OsMADS25 modulates root growth and confers salinity tolerance via the ABA-mediated regulatory pathway and ROS scavenging [J]. Plos Genetics, 2018, 14(10): e1007662.
[37] Zhang D, Zhang M, Zhou Y, Wang Y, Shen J, Chen H, Zhang L, Lu B, Liang G, Liang J. The rice G protein γ subunit DEP1/qPE9–1 positively regulates grain-filling process by increasing auxin and cytokinin content in rice grains [J]. Rice, 2019, 12(1): 91.
[38] Rubio S, Noriega X, Perez F J. ABA promotes starch synthesis and storage metabolism in dormant grapevine buds [J]. Journal of Plant Physiology, 2019, 234: 1–8.

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Comparison of Physiological and Biochemical Properties of Storage Roots Between High and Low Starch Cultivars of Cassava during the Critical Growth Period

木薯高、低粉品种贮藏根生长关键期的生理生化特性比较

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