Abstract: The contents of lactone glycosides in Goodyera schlechtendaliana and its related species from different habitat were compared in this study to provide reference for the quality control of Goodyera. By HPLC–ELSD method, ChromCore AQ C18 (3 μm, 4.6 mm×250 mm) was used as the chromatographic column, and ultrapure water was used as the mobile phase. The flow rate was 0.5 mL·min–1, the column temperature was 30 ℃, and the atomization temperature of evaporative light scattering detector was 70 ℃. The flow rate of carrier gas was 2.5 mL·min–1, and the sample volume was 5 μL. The content of lactone glycosides in 30 batches of G. schlechtendaliana and its related species from different habitat was determined. The linear ranges of goodyeroside A and kinseoside were 62.5–1500 μg·mL–1 (r=0.9995) and 62.5–1500 μg·mL–1 (r=0.9996) respectively. The RSD of precision, repeatability and stability were less than 3%, and the average recovery rates were 101.41% and 101.16% respectively. The contents of goodyeroside A in 30 batches of G. schlechtendaliana and its related species were in the range of 28.31–243.07 mg·g–1, and the average content of 134.31 mg·g–1, which was quite different (RSD= 38.40%). The contents of kinseoside in 30 batches of G. schlechtendaliana and its related species were in the range of 0–184.37 mg·g–1, and the average content of 34.50 mg·g–1, and there were also great differences (RSD=141.30%). The method was simple, rapid, stable and reproducible, with good precision and accuracy, and be used for the content analysis of lactone glycosides in G. schlechtendaliana and its related species.

Key words: Goodyera schlechtendaliana; goodyeroside A; kinseoside; content determination

摘要： 比较不同产地斑叶兰Goodyera schlechtendaliana及近缘种植物中两种内酯苷类成分的含量，为斑叶兰药材的质量控制提供参考。采用HPLC-ELSD法，以ChromCore AQ C18 (3 μm，4.6 mm×250 mm)为色谱柱，超纯水为流动相，等度洗脱；流速0.5 mL·min–1，柱温30 ℃，蒸发光散射检测器雾化温度70 ℃；载气流速2.5 mL·min–1，进样量5 μL，测定30批不同产地斑叶兰及近缘种内酯苷类成分含量。结果表明，斑叶兰苷和金线莲苷含量的线性范围均为62.5～1500 μg·mL–1，相关系数r分别为0.9995、0.9996，精密度、重复性、稳定性的RSD均小于3%；平均加样回收率分别为101.41%和101.16%，RSD分别为1.53%和1.36%。30批斑叶兰及近缘种植物的斑叶兰苷含量在28.31～243.07 mg·g–1之间，平均含量为134.31 mg·g–1，存在较大的差异(RSD=38.40%)；30批斑叶兰及近缘种植物的金线莲苷含量在0～184.37 mg·g–1之间，平均含量为34.50 mg·g–1，也存在较大的差异(RSD=141.30%)。该方法操作简便、快捷、稳定性和重复性良好，具有较好的精密度和准确度，可用于斑叶兰及近缘种内酯苷类成分含量分析。

关键词: 斑叶兰；斑叶兰苷；金线莲苷；含量测定