Influence of Different Elicitors on Cell Growth, Ursolic Acid and Oleanic Acid Content of Loquat Cell Suspension Culture

doi:10.3969/j.issn.1009-7791.2014.03.001

Subtropical Plant Science ›› 2014, Vol. 43 ›› Issue (03): 187-191.DOI: 10.3969/j.issn.1009-7791.2014.03.001

Influence of Different Elicitors on Cell Growth, Ursolic Acid and Oleanic Acid Content of Loquat Cell Suspension Culture

LI Hui-hua,LIU Xiao-ying,XU Jian,CHANG Qiang,WANG Wei,SU Ming-hua

(Fujian Key Laboratory of Subtropical Plant Physiology and Biochemistry, Fujian Institute of Subtropical Botany, Xiamen 361006, Fujian China)

Received:2014-05-04 Revised:2014-05-04 Online:2014-09-10 Published:2014-09-10
Contact: SU Ming-hua

不同诱导子对枇杷悬浮细胞生长及熊果酸、齐墩果酸含量的影响

李惠华,刘小英,徐剑,常强,王伟,苏明华

（福建省亚热带植物研究所，福建省亚热带植物生理生化重点实验室，福建厦门 361006）

通讯作者: 苏明华
作者简介:李惠华，博士，副研究员，从事植物生理与分子生物学研究。
基金资助:
福建省自然科学基金(2012J05047)、福建省公益类科研院所专项(2011R1012-1)、厦门市科技计划项目(3502Z20132004)

Abstract

Abstract: Using the suitable loquat cell suspension culture as test materials, the influence of different elicitors (salicylic acid, SA; jasmonic acid, MJ; casein acid hydrolysate, CH; yeast extract, YE) on the cell growth, the accumulation of ursolic acid (UA) and Oleanic acid (OA) were studied. The results showed that the addition of MJ 100.0 mg.L^-1 was advantageous to loquat cell suspension culture, the fresh weight, UA and OA content, were 1.081, 2.540, 2.590 times to the control, respectively.

Key words: loquat, cell suspension, elicitor, ursolic acid, oleanic acid

摘要： 在已建立的稳定枇杷细胞悬浮培养系的试验中，研究加入诱导子水杨酸（SA）、茉莉酸甲酯（MJ）、水解酪蛋白（CH）和酵母提取物（YE）对枇杷悬浮细胞生长及次生代谢产物熊果酸（UA）、齐墩果酸（OA）含量的影响。结果表明，从细胞生长、UA及OA含量三方面综合考虑，以MJ为100.0 mg.L^-1时培养最佳，此时细胞生长、UA及OA含量分别为对照的1.081、2.540及2.590倍。

关键词: 枇杷, 细胞悬浮培养, 诱导子, 熊果酸, 齐墩果酸

CLC Number:

Q943.1

LI Hui-hua,LIU Xiao-ying,XU Jian,CHANG Qiang,WANG Wei,SU Ming-hua. Influence of Different Elicitors on Cell Growth, Ursolic Acid and Oleanic Acid Content of Loquat Cell Suspension Culture[J]. Subtropical Plant Science, 2014, 43(03): 187-191.

李惠华,刘小英,徐剑,常强,王伟,苏明华. 不同诱导子对枇杷悬浮细胞生长及熊果酸、齐墩果酸含量的影响[J]. 亚热带植物科学, 2014, 43(03): 187-191.

References

[1] 严华,苏健,王宝琹. 药材中齐墩果酸与熊果酸质控指标的探究[J]. 药物分析杂志, 2009(8): 1400—1406.

[2] 王汉屏,王立志. 木瓜齐墩果酸的研究进展[J]. 食品科学, 2007(10): 621—624.

[3] Lee I, Lee J, Lee Y H, Leonard J. Ursolic acid-induced changes in tumor growth, O2 consumpition, and tumor interstitial fluid pressure[J]. Anti-cancer Research, 2001,21(4A): 2827—2833.

[4] Lee H Y, Chang H Y, Kim K H, Lee J J, Kim K W. Induction of differentiation in the cultured F9 terato carcinoma stem cells by triterpene acids[J]. Cancer Research Clinical Nocology, 1994,120(9): 516—523.

[5] Chiang L C, Chiang W, Chang M Y, Ng L T, Lin C C. Anti-leukemic activity of selected natural products in Taiwan[J]. American Journal Chinese Medicine, 2003,31(1): 37—46.

[6] Li H J, Guo W J, Yang Q Y. Effects of ursolic acid and oleanolic acid on human colon carcinom a cell line HCT15[J]. World Journal Gastroenterology, 2002,8(3): 493.

[7] Kim D K, Baek J H, Kang C M, Yoo M A, Sung J W, Kim D K, Chung H Y, Kim N D, Choi Y H, Lee S H, Kim K W. Apoptotic activity of ursolic acid may correlate with the inhibition of initiation of DNA replication[J]. International Journal Cancer, 2000,87(5): 629.

[8] 相延英,杨光. 常用中药中齐墩果酸和熊果酸的含量测定[J]. 中国医院药学杂志, 2004,24(5): 316—318.

[9] Georgiew M I, Weber J, Maciuk A. Bioprocessing of plant cell cultures for mass production of targeted compounds[J]. Applied Microbiology Biotechnology, 2009,83: 809—823.

[10] Mustafa N R, Winter W, Iren F, Verpoorte R. Initiation, growth and cryopreservation of plant cell suspension cultures[J]. Nature Protocols, 2011,6(6): 715—742.

[11] 司徒琳莉,袁长友. 次生代谢产物: 代谢途径、分类、作用及其生产[J]. 牡丹江师范学院学报(自然科学版), 2001,23(3): 3—5.

[12] 杜丽娜,张存莉,朱玮,张高宏. 植物次生代谢合成途径及生物学意义[J]. 西北林学院学报, 2005,20(3): 150—155.

[13] 赵鸿莲,于荣敏. 诱导子在植物细胞培养中的应用研究进展[J]. 沈阳药科大学学报, 2000,17(2): 152—156.

[14] 高丽君,崔建华,刘风云,尹迎春. 植物次生代谢物的应用和开发[J]. 生物学通报, 2004,39(7): 15—17.

[15] Malamy J, Carr J P, Klessig D F, Raskin L. Salicylic acid: A likely endogenous signal in the resistance response of tobacco to viral infection[J]. Science, 1990,250(4983): 1002—1004.

[16] 刘新. 水杨酸对植物生理活动的影响[J]. 莱阳农学院学报, 1998,15(4): 280—284.

[17] 滕艳萍,梁宗锁. 诱导子在药用植物中的应用[J]. 西北农林科技大学学报(自然科学版), 2006,34(4): 91—95.

[18] 李忠光,龚明. 水解酪蛋白对烟草愈伤组织和悬浮培养细胞生长的促进作用[J]. 云南师范大学学报, 2006,26(4): 60—61.

[19] 张春荣,李玲. 水杨酸、茉莉酸甲酯和乙烯利对野葛细胞悬浮培养生产葛根素的影响[J]. 植物资源与环境学报, 2003,12(1): 56—57.

[20] 王宁宁,王淑芳,王勇,田俊英,朱亮基. 外源刺激物对长春花冠瘿细胞生长和吲哚生物碱含量的影响[J]. 生物工程学报, 1996,11(S1): 1—4.

[21] 徐亮胜,薛晓锋,付春祥,金治平,陈毓荃,赵德修. 茉莉酸甲酯与水杨酸对肉苁蓉悬浮细胞中苯乙醇甙合成的影响[J]. 生物工程学报, 2005,21(3): 402—406.

[22] 吕寒,习超鹏,陈剑,李维林,张涵庆. 不同产地枇杷叶中三萜酸类成分的含量比较[J]. 时珍国医国药, 2000,21(12): 3170—3171.

[23] 兰文智,余龙江,吴元喜. 水杨酸对真菌诱导子诱导红豆杉悬浮细胞膜脂过氧化和紫杉醇生物合成的影响[J]. 生物工程学报, 1996,12(增刊): 300—303.

[24] Suzuki H, Achnine L, Xu R, Matsuda S, Dixon R A. A genomics approach to the early stages of triterpene saponin biosynthesis in Medicago truncatula[J]. The Plant Journal, 2002,32(6): 1033—1048.

[25] 宁文,曹日强. 促进紫草色素合成的米曲霉诱导子的纯化和活性鉴定[J]. 植物学报, 1996,38(5): 367—374.

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Influence of Different Elicitors on Cell Growth, Ursolic Acid and Oleanic Acid Content of Loquat Cell Suspension Culture

不同诱导子对枇杷悬浮细胞生长及熊果酸、齐墩果酸含量的影响

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