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10 September 2014, Volume 43 Issue 03 Previous Issue    Next Issue
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Influence of Different Elicitors on Cell Growth, Ursolic Acid and Oleanic Acid Content of Loquat Cell Suspension Culture LI Hui-hua,LIU Xiao-ying,XU Jian,CHANG Qiang,WANG Wei,SU Ming-hua 2014, 43(03): 187-191.  DOI: 10.3969/j.issn.1009-7791.2014.03.001 Abstract ( )   PDF (286KB) ( )   Using the suitable loquat cell suspension culture as test materials, the influence of different elicitors (salicylic acid, SA; jasmonic acid, MJ; casein acid hydrolysate, CH; yeast extract, YE) on the cell growth, the accumulation of ursolic acid (UA) and Oleanic acid (OA) were studied. The results showed that the addition of MJ 100.0 mg.L-1 was advantageous to loquat cell suspension culture, the fresh weight, UA and OA content, were 1.081, 2.540, 2.590 times to the control, respectively. References | Related Articles | Metrics

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Microbial Transformation of Gynostemma pentaphyllum from Fujian by Fungi and Its Anticancer Activity CHEN Liang-hua,ZHENG Zhi-zhong,MING Yan-lin,LIU Shao-song,TONG Qing-xuan 2014, 43(03): 192-196.  DOI: 10.3969/j.issn.1009-7791.2014.03.002 Abstract ( )   PDF (346KB) ( )   Microbial transformation of Gynostemma pentaphyllum from Fujian by two fungi of Aspergillus niger and A. nidulans was studied. After gypenosides being fermented in liquid for 2 d using the foregoing fungi at 37 ℃, HPLC analysis of the transformed products of gypenoside via microorganism indicated that the composition of the fermentation broth changed obviously. To compare the activity of transformed substrate and products of gypenosides, the anticancer activity was evaluated by MTT assay. The results showed that the transformed products of gypenoside via microorganism exhibited the obvious cytotoxic activity on human hepatoma cells SMCC7721 and Bel7402, but the transformed substrate of gypenosides was inactive. Products transformed by A. nidulans and A. niger had inhibitory effect on the growth of SMCC7721 cells for 72 h with the 50% inhibitory concentration (IC50) of 92.00 and 40.60 mg·mL-1 respectively, with 49.06 and 125.38 mg·mL-1 on the growth of Bel7402 cells. The results indicated that microbial transformation of the gypenosides from G. pentaphyllum via two fungi had produced the anticancer activity constitutes. The research provided new research ideas for the later works of bioscreening and separation of active ingredients from G. pentaphyllum. References | Related Articles | Metrics

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Study on HPLC Fingerprint Analysis of Clerodendranthus spicatus LI Jin-yu,CHEN Chun,LIN Jian-zhong,HU Wei-ji,XU Wen-jiang 2014, 43(03): 197-201.  DOI: 10.3969/j.issn.1009-7791.2014.03.003 Abstract ( )   PDF (455KB) ( )   To establish a method of fingerprint analysis on Clerodendranthus spicatus by HPLC, the chromatographic fingerprint was obtained by Phenomenex Synergi 4u hydro-RP 250×4.60 mm with the gradient elution solvent system composed of methanol-0.1% formic acid. The detective wavelength was 254 nm; the flow rate was 1.0 mL·min-1 and the column temperature was maintained at 40 ℃. The results showed that 15 co-possessing peaks were selected as the fingerprint peaks of C. spicatus and good similarities with correlation coefficients higher than 0.9 were found in fingerprints between the different samples of C. spicatus and the standard fingerprint. The method with good reproducibility was simple and accurate, it could be used as a effective quality control method for C. spicatus. References | Related Articles | Metrics

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Optimization of Separation and Purification Procedure of Total Flavonoids in Pyrrosia petiolosa by Macroporous Adsorption Resin LIU Jing,CHENG Dan-dan,XIN Xiao-wei,GAO De-min,ZHANG Hong-meng 2014, 43(03): 202-205.  DOI: 10.3969/j.issn.1009-7791.2014.03.004 Abstract ( )   PDF (214KB) ( )   To select the optimum purifying process of total flavonoids in Pyrrosia petiolosa, orthogonal experiment was designed with the desorption rate of total flavonoids and the content of total flavonoids as indexes. The results showed that the best separation conditions were determined as: sample concentration 0.5 mg.mL-1 at pH 6, eluent concentration 50%, elution rate 2 mL.min-1 by D-101 macroporous resin. The concentration of total flavonoids in the filtrate could reach 76.40%, and the method was stable and feasible, and the result was reliable. References | Related Articles | Metrics

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Optimization of Protein Extraction and Two-dimensional Electrophoresis Conditions of the Phalaenopsis Leaves ZHU Xiao-shu,YE Qing-sheng 2014, 43(03): 206-211.  DOI: 10.3969/j.issn.1009-7791.2014.03.005 Abstract ( )   PDF (1334KB) ( )   A two-dimensional electrophoresis（2-DE）system for proteomic analysis of Phalaenopsis leaves was established by optimizing the extraction methods, parameters including the pH gradient of IPG strips, sample loading, hydration ways and isoelectric focusing conditions. The optimized system included the following steps: extracting the total protein from Phalaenopsis leaves by phenol method, separating the proteins with 24 cm pH 3—10 NL IPG strips, loading protein samples of 1.35 mg by passive hydration followed by isoelectric focusing program B1, and staining the gels by Coomassie Brilliant Blue after SDS-PAGE (12%) electrophoresis. On the basis of optimized 2-DE system of Phalaenopsis leaves, reproducible profiles with high resolution were obtained. References | Related Articles | Metrics

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Optimization Conditions of Cymbidium hybridum Protoplast Isolation XU Chuan-jun,CHEN Yi-hui,LI Ai-zhen,HUANG Jun-mei,ZENG Bi-yu,HUANG Wen 2014, 43(03): 212-215.  DOI: 10.3969/j.issn.1009-7791.2014.03.006 Abstract ( )   PDF (295KB) ( )   Taking Cymbidium hybridum leaves and roots as material, the effects of enzyme combination, shaking speed, enzymolysis time, enzymolysis temperature, and the ratio of materials enzyme on protoplast isolation were studied. The results showed that：after 90 min pretreatment for plasmolysis, protoplast yield from leaves was the highest using mixed enzyme (2.0% cellulose + 0.1% pectinase + 0.2% macerozyme + 0.2% driselase) with shaking speed 50 r·min-1 at 28 ℃ for 6 h. And protoplast yield from roots was the highest using mixed enzyme (2.0% cellulose + 0.3% pectinase + 0.1% macerozyme + 0.1% driselase) under the same conditions. References | Related Articles | Metrics

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Effect of Soil’s Micronutrient Element on the Quality of Wuyi Rougui Tea CHEN Hua-kui,YANG Jiang-fan 2014, 43(03): 216-221.  DOI: 10.3969/j.issn.1009-7791.2014.03.007 Abstract ( )   PDF (340KB) ( )   Analysing chemical compounds of Rougui tea cultivar fresh leaves planted in different place, we found that polyphenol compounds, amino acids and caffeine of the tea trees in Mingyan are higher than those of tea trees planted in Danyan. So that the fresh leaves in Mingyan are better than Danyan. Rougui tea produced in Mingyan comparing with that produced in Danyan, polyphenol compounds, amino acid and caffeine of Rougui tea produced in Mingyan are higher, but other compounds are no significant differences. Analysing trace elements, we found that copper are significant correlation between fresh leaves and tea product. Copper of fresh leaves in Mingyan are higher than Danyan. Conversely, copper and zinc in soil of Danyan are significant higher than that of Mingyan, iron and manganese are no significant difference. References | Related Articles | Metrics

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Dynamics of Flower and Fructification of Three Dominant Species in the Baishanzu Forest, Zhejiang Province WU Meng-si,ZHOU Li-meng,XU Da-ming,DING Bing-yang 2014, 43(03): 222-226.  DOI: 10.3969/j.issn.1009-7791.2014.03.008 Abstract ( )   PDF (587KB) ( )   This study describes the dynamics of flower and fructification of three dominant species, including Rhododendron latoucheae, Camellia cuspidata and Schima superba, from 2009 to 2012 in Baishanzu evergreen broad-leaved forest. The results show that: 1) From the analysis of temporal dynamics of flowers, R. latoucheae and S. superba have significant in-and-off year phenomena of flower in the plot, while C. cuspidata doesn't. 2) From the analysis of temporal dynamics of fructification, R. latoucheae has significant in-and-off year phenomena of fruits, but C. cuspidata and S. superba don't. 3) From the analysis of seasonal variation of flowers, R. latoucheae and C. cuspidata littered in the late spring, whereas S. superba littered in the early fall. 4) Abnormal litter of fructification has a closed contact with unfavorable weather. References | Related Articles | Metrics

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Behavior and Activity Rhythm of Flower-visiting Insects on Buddleja davidii SHAO Chang-fen,GUAN Wen-ling,LI De-fa,LI Ye-fang 2014, 43(03): 227-231.  DOI: 10.3969/j.issn.1009-7791.2014.03.009 Abstract ( )   PDF (293KB) ( )   The species and visiting behavior of flower-visiting insects on Buddleja davidii were investigated during flowering period in Yunnan Agricultural University. There were 63 species of flowering-visiting insects on B. davidii, belonging to Diptera, Hemiptera, Hymenopter, Lepidoptera, Coleoptera, Dermaptera and Mantodea. The main flower-visiting insect on Buddleja davidii was syrphid flies. The species of flower-visiting insects was differed among different month. The species and quantity of flower-visiting insect was effected by temperature changing. The flower-visiting frequency of flower-visiting insects to B. davidii was different, with the highest flower-visiting frequency from 12:00 to 16:00 everyday. References | Related Articles | Metrics

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Investigation on Wild Resources of Caryota maxima in the Northernmost Distribution in China GUO Hui-zhu,CHEN Heng-bin,RUAN Zhi-ping,CHEN Xiao-hui,CAI Bang-ping,HUANG Quan-neng,LIU Yu-ming 2014, 43(03): 232-235.  DOI: 10.3969/j.issn.1009-7791.2014.03.010 Abstract ( )   PDF (315KB) ( )   During a survey of wild resources of Palmae in China, Caryota maxima was first found in Longhai county, Zhangzhou city of Fujian province, and the investigation of distribution, habitat and growth situation of the wild C. maxima was carried out. The results show that, firstly, Longhai county in Zhangzhou city is the northernmost and easternmost known natural distribution of C. maxima in China; Secondly, C. maxima grows well in the natural distribution, flowering or fruiting plants and plantlets are found and indicates that the offspring could regenerate in the area; Thirdly, the area distribution of C. maxima is narrow and sporadic, and the wild resources are in endangered, so they should be protected. References | Related Articles | Metrics

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Listera——A Newly Recorded Genus of Orchidaceae from Zhejiang Province LIU Ri-lin,MEI Zhong-hai,ZHANG Fen-yao,XIE Wen-yuan,CHEN Fen 2014, 43(03): 236-237.  DOI: 10.3969/j.issn.1009-7791.2014.03.011 Abstract ( )   PDF (206KB) ( )   Listera japonica and the genus Listera (Orchidaceae) were recorded as geographic distribution new to Zhejiang Province, which were found in Wangdongyang alpine wetland nature reserve of Jingning County. The voucher specimens were preserved in the herbarium of Zhejiang Agriculture and Forestry University (ZJFC). References | Related Articles | Metrics

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Residual Dynamics and Safe Evaluation of Fenvalerate in Bananas and Its Orchard Soil CHEN Li-ping 2014, 43(03): 238-241.  DOI: 10.3969/j.issn.1009-7791.2014.03.012 Abstract ( )   PDF (275KB) ( )   The residue dynamics of fenvalerate in bananas and the soil were studied by using the methods of GC and field experiments. The results showed that the original sediment of fenvalerate in bananas was significant differences among different treatments. Fenvalerate was degrading at a slow rate in bananas and its soil. The residual degradation dynamics of fenvalerate degradation in bananas and its orchard soil could be followed first order kinetic equation ‘C＝C0·e-kt’. In bananas, the correlation coefficient (r) ranged from 0.9722 to 0.9980 (P < 0.01), the degradation coefficient (k) ranged from 0.0703 to 0.0767, T1/2 ranged from 9.1 d to 9.9 d and T0.99 ranged from 60.1 d to 65.6 d. On the other hand, r＝0.9877 (P＜0.01), k＝0.0821, T1/2＝8.5 d and T0.99＝56.1 d in its soil. Furthermore, after fenvalerate secondly being applied for 62—68 d, the residue of fenvalerate in banana was < 0.01 mg·kg-1, and that in orchard soil was from 0.015 to 0.020 mg·kg-1. The application of fenvalerate into bananas is done according to the regular method in Fujian with an effective dose of 112.50 g·hm-2, and applied twice with an interval of 7 d which means that >13.6 d can be recommended as pre-harvest time. The quality safety of their products was fulfilled quality requirements of GB/T 2763-2012 and NY/T 750-2011. References | Related Articles | Metrics

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Inhibition of Exopleura Extract of Ginkgo biloba on Pathogens from Several Kinds of Vegetables LIN Qing-hong,LI Jin-yu,LIN Zhi-kai,HUANG Yu-huan,XU Wen-jiang,LIN Guang-rong 2014, 43(03): 242-245.  DOI: 10.3969/j.issn.1009-7791.2014.03.013 Abstract ( )   PDF (312KB) ( )   Inhibition of extract from exopleura of Ginkgo biloba on pathogens from several kinds of vegetables was conducted. The results showed that inhibition rates of extract of ethanol and methanol against the pathogens were the best. The inhibition rates of extract of ethanol at 500 mg.mL-1 on the Colletotrichum higgisianum, Rhizoctonia solani, Pellicularia rolfsii, Fusarium oxysporum f. sp. cucrmerinum were 100%, those of methanol at 500 mg.mL-1 on the Alternaria brassicae, Colletotrichum higgisianum, Rhizoctonia solani, Pellicularia rolfsii, Fusarium oxysporum f. sp. cucrmerinum were also 100%. References | Related Articles | Metrics

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Comprehensive Evaluation and Analysis of Common Garden Tree Species in Jinjiang City, Fujian Province ZHUANG Shu-yuan 2014, 43(03): 246-250.  DOI: 10.3969/j.issn.1009-7791.2014.03.014 Abstract ( )   PDF (306KB) ( )   The common trees applied for many years in built-up area of Jinjiang City, Fujian were investigated. To understand their growth in Jinjiang, evaluation and analysis of stress resistance, ornamental effect, ecology, dangers, maintenance and other indicators were carried out. The results indicated that the numbers of class Ⅰ, Ⅱ, Ⅲ and Ⅳ were 8, 17, 17 and 7, respectively. Class Ⅰ, including Ficus microcarpa, Syzygium cumini, Cinnamomum camphora, Ficus virens var. sublanceolata, Ficus altissima, Khaya senegalensis, Alstonia scholaris and Ficus religiosa, could be widely used in urban landscape for their high ecological functions and ornamental effect. References | Related Articles | Metrics

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Artificial燘reeding of Some Native燜ragrant Plants in Xiamen, Fujian Province LIU Fu-ping,SONG Zhi-yu,ZHANG Xiao-hang 2014, 43(03): 251-254.  DOI: 10.3969/j.issn.1009-7791.2014.03.015 Abstract ( )   PDF (307KB) ( )   To play the effects of native fragrant plants of Xiamen on perfuming environment, four kinds of ornamental fragrant plant were tested on artificial breeding for improving their reproductive efficiency. In accelerating germination experiment of Michelia maudiae, it worked best that seeds were soaked with 300 mg·L-1GA3 at 50℃, the germination rate and germination potentiality were increased by 22.7% and 17.3%, respectively,  as compared?to that of peeled seeds. The seed of Vitex trifolia var. simplicifolia which was accelerated germination with 1.0 g·L-1 GA3 at 50 ℃ had a best result, germination rate and germination potentiality were increased by 46.7% and 25.4%, respectively, than the control. In cuttage test the cuttings were pretreatmented with some plant growth regulators, and observed rooting rate, survival rate and average root number per cutting. Increase rates of three indexes were highest when Gardenia jasminoides cuttings pretreatmented with the solution of 100 mg·L-1 NAA and 100 mg·L-1 IBA, rooting rate and survival rate were increased by 18.7% and 15.8%, respectively, comparing?with the control, and the average root number per cutting were increased ?by 58.9%. It had the?best?] cuttage effect  that Trachelospermum jasminoides cuttings soaked with the solution of 200 mg·L-1 NAA and 200 mg·L-1 IBA, the increase rates of rooting rate and survival rate were 24.5% and 19.1% respectively, compared to the control, and the increase rates of average root number per cutting was 66.7%. References | Related Articles | Metrics

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Study on Rapid Propagation in vitro of Two Cultivars of Nandina domestica WANG Hua-yu,LI Li-feng,CHEN Yan,CHEN Nai-ming 2014, 43(03): 255-258.  DOI: 10.3969/j.issn.1009-7791.2014.03.016 Abstract ( )   PDF (320KB) ( )   The stems and stem apexes of Nandina domestica ‘Firepower’ and N. domestica ‘Harbour Dwarf’ were used as explants to study the techniques of adventitious bud induction, proliferation and rooting. The results showed that the best medium for proliferation of two cultivars was MS + 6-BA 1.0 mg·L-1 + NAA 0.1 mg·L-1. The rooting rate of N. domestica ‘Harbour Dwarf’ could be as high as 100% in rooting medium 1/2MS + NAA 0.1 mg·L-1 + IBA 0.1 mg·L-1, while only 73.5% for the other cultivar. Plantlets in propagation stage were cultivated for 15 days in the root medium, and then transplanted to coconut chaff in plastic containers which were sterilized by γ-ray. In the coconut chaff, the rooting rate of ‘Firepower’ could be increased to 92.0%, and the plantlets grew well. References | Related Articles | Metrics

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Study on Callus Induction of Dendrobium officinale YANG Rui-juan,WANG Qiao-mei,YAN Liang, 2014, 43(03): 259-261.  DOI: 10.3969/j.issn.1009-7791.2014.03.017 Abstract ( )   PDF (277KB) ( )   The effect of different culture media and lighting conditions on callus induction of Dendrobium officinale was conducted. The result indicated that the optimal culture condition for full explants was MS 5.92 g·L-1 + 2,4-D 5 mg·L-1 + IAA 1.5 mg·L-1 + KT 0.62 mg·L-1 + sucrose 37.5 g·L-1 + agar 0.8% (pH 5.9～6.0), keeping 15 days in dark and then incubating under light．The optimal culture condition for mashed explants was MS 4.74 g·L-1 + 2,4-D 1 mg·L-1 + IAA 1.5 mg·L-1 + KT 0.25 mg·L-1 + sucrose 30 g·L-1 + agar 0.8% (pH 5.9～6.0), incubating under light at 25 ℃. References | Related Articles | Metrics

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Rapid Propagation and in vitro Conservation of Fosbergia shweliensis LI Ai-hua,CHENG Zhi-ying,YANG Xiang-yun 2014, 43(03): 262-263.  DOI: 10.3969/j.issn.1009-7791.2014.03.018 Abstract ( )   PDF (252KB) ( )   The techniques of tissue culture and in vitro conservation of Fosbergia shweliensis were studied. The results showed the aseptic plantlets could be obtained from stem tip of seedlings and stem node explants of F. shweliensis. Their suitable initial medium was MS medium with 3.0 mg·L-1 6-BA, 0.1 mg·L-1 NAA and 3% sucrose, and MS medium with 2.0 mg·L-1 6-BA, 0.1 mg·L-1 NAA and 3% sucrose, respectively. The multiplication rate was up to 3—4 times. The best rooting for plantlets occurred on 1/2MS medium with 1.0 mg·L-1 IBA and 2% sucrose, the rooting rate was 100%. The survival rate of aseptic plantlets transplanted after hardening was more than 90%. The tube-test plantlets could grow slowly on 1/2MS medium with 3% sucrose at 12—15 ℃, in consequence subculture period was lengthened to 18—24 months. The success of rapid propagation and in vitro conservation supported the further research, development and utilization of this species. References | Related Articles | Metrics

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Cross Breeding Test of Cycas sexseminifera and C. panzhihuaensis PAN Ai-fang 2014, 43(03): 264-265.  DOI: 10.3969/j.issn.1009-7791.2014.03.019 Abstract ( )   PDF (215KB) ( )   Artificial pollination between Cycas sexseminifera (the male) and C. panzhihuaensis (the female) was carried out．The results showed that these two species of cycads can interspecific hybridization, but the pollination need pollinators. References | Related Articles | Metrics

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Review on Crop Space Breeding and Industrialization Promotion CHENG Xiao-bing 2014, 43(03): 266-270.  DOI: 10.3969/j.issn.1009-7791.2014.03.020 Abstract ( )   PDF (244KB) ( )   The concept and development of crops space breeding, breeding programs, its biosafety and industrialization promotion are reviewed. The biological safety of space breeding, physical mutation breeding and transgenic breeding are compared. The problems of space seed research and its industrialization promotion are revealed, and some solutions are suggested. References | Related Articles | Metrics