Abstract: The morphology and tissue structure of Goodyera brachystegia were studied by pharmacognosy identification method, and the contents of its main components, including kinsenoside, total flavonoids and polysaccharides, were determined by HPLC-ELSD method and UV spectrophotometry. The leaves of G. brachystegia are elliptical or ovate, with green upper surface without white spots and light green underside. The flowers are small, with white petals, oblique rhombus-shaped oblanceolate. The lip is broadly ovate, with light yellow base, and concave into a hemispherical hood. Microscopic identification shows that the ventilation tissue of the root cross-section is well developed, and the vascular bundles are arranged in a radial pattern of multiple archetypes. The vascular bundles of the stem are scattered into a finite external tough type. The lower epidermis of the main vein of the leaves is semicircular and raised, and the stomata of the lower epidermal are mainly indefinite. The average contents of kinsenoside, total flavonoids and polysaccharides in two batches of G. brachystegia were 14.90%, 1.52% and 2.05%, Among them, kinsenoside is a distinctive chemical marker that differentiates G. brachystegia from other Goodyera species. The above characteristics can provide a reference for the identification, resource development and utilization of G. brachystegia.

Key words: Goodyera brachystegia, pharmacognosy identification, kinsenoside, total flavonoids, polysaccharides

摘要： 采用生药学鉴定方法对莲座叶斑叶兰Goodyera brachystegia植物形态和组织构造进行研究，并应用HPLC-ELSD法和紫外分光光度法测定其主要成分金线莲苷、总黄酮和多糖含量。结果表明，莲座叶斑叶兰叶片椭圆形或卵形，叶上表面绿色，无白色斑纹，叶背面淡绿色；花较小，花瓣白色，斜菱状倒披针形；唇瓣宽卵形，基部淡黄色，凹陷成半球形兜状；显微鉴定表明，根横切面通气组织发达，维管束多原型辐射状排列；茎维管束散生为有限外韧型；叶主脉下表皮呈半圆形隆起且下表皮气孔以不定式为主。两批次莲座叶斑叶兰中金线莲苷、总黄酮、多糖的平均含量分别是14.90%、1.52%、2.05%，其中金线莲苷是区别莲座叶斑叶兰与其他斑叶兰属植物的差异性化学标志物。上述特征可为莲座叶斑叶兰的鉴定、资源开发利用提供参考依据。

关键词: 莲座叶斑叶兰, 生药学鉴定, 金线莲苷, 总黄酮, 多糖