Effects of Different Drying Methods on the Contents of 7 Polyphenols in Tetrastigma
hemsleyanum Leaves

doi:10.3969/j.issn.1009-7791.2025.06.003

Subtropical Plant Science ›› 2025, Vol. 54 ›› Issue (6): 621-628.DOI: 10.3969/j.issn.1009-7791.2025.06.003

• Research articles • Previous Articles Next Articles

Effects of Different Drying Methods on the Contents of 7 Polyphenols in Tetrastigma hemsleyanum Leaves

LIN Mian-fa, FU Deng-ke, YANG Nan, HONG Yu-yan, PAN Meng-ting, FAN Shi-ming, XU Wen, XU Hui-long^*

(College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian China)

Online:2025-12-31 Published:2025-12-31
Contact: XU Hui-long

不同干燥方式对三叶青叶片7种多酚类成分含量的影响

林绵发，付登科，杨楠，洪玉燕，潘梦婷，范世明，许文，徐惠龙^*

(福建中医药大学药学院，福建福州 350122)

通讯作者: 徐惠龙
基金资助:
福建省自然科学基金项目(2022N5018)；福建省自然科学基金项目(2024L3013)；厦门市科技特派员专项(20222004)；福建中医药大学校管课题(X2023031-重点)

Abstract

Abstract: The effects of different drying methods on the content of polyphenolic components in Tetrastigma hemsleyanum leaves were investigated using ultra-high performance liquid chromatography (UHPLC) to provide an experimental basis for its processing methods. The contents of neo-chlorogenic acid, chlorogenic acid, 3-O-p-coumaroylquinic acid, isoorientin, orientin, isovitexin, and vitexin were determined from the leaves with three drying methods: shade-drying, oven-drying (40, 60, 80 ℃), and freeze-drying (–20 ℃). The drying methods were then comprehensively analyzed using the entropy weight-TOPSIS method. The validation results of content determination methodology were good, and the content ranges of seven ployphenols components (neo-chlorogenic acid, chlorogenic acid, 3-O-p-coumaroylquinic acid, isoorientin, orientin, isovitexin, vitexin) in T. hemsleyanum were 0.67–5.39, 1.05–8.12, 0.22–0.63, 0.60–1.38, 0.93–1.37, 0.38–0.66, 0.56–1.21 mg·g^–1, respectively. The total contents of ployphenol, phenolic acids (neo-chlorogenic acid, chlorogenic acid, 3-O-p-coumaroylquinic acid), and flavonoids (isoorientin, orientin, isovitexin, vitexin) ranged from 4.47 to 18.15 mg·g^–1, 1.94 to 14.14 mg·g^–1, and 2.53 to 4.41 mg·g^–1, respectively. Entropy weight-TOPSIS analysis showed that drying at 80 ℃ was suitable for T. hemsleyanum leaf drying. The established method is accurate, stable, and reliable; the different drying methods significantly affect the ployphenol content in T. hemsleyanum leaves, with hot-air drying at 80 ℃ likely representing the optimal drying approach. This finding provides valuable reference for subsequent research on fresh-processing techniques of T. hemsleyanum leaves.

Key words: Tetrastigma hemsleyanum, UHPLC, drying methods, entropy weight-TOPSIS, content determination

摘要： 考察不同干燥方式对三叶青Tetrastigma hemsleyanum叶片多酚类成分含量的影响，为其加工方法提供科学依据。采用超高液相色谱测定阴干、烘干(40、60、80 ℃)和冻干(–20 ℃)三种干燥方式提取物中新绿原酸、绿原酸、3-O-对香豆酰基奎宁酸、异荭草素、荭草素、异牡荆素、牡荆素含量，并结合熵权-TOPSIS对各干燥方式进行综合分析。结果表明，含量测定方法学验证结果良好，7种多酚类成分(新绿原酸、绿原酸、3-O-对香豆酰基奎宁酸、异荭草素、荭草素、异牡荆素、牡荆素)含量范围分别为0.67～5.39、1.05～8.12、0.22～0.63、0.60～1.38、0.93～1.37、0.38～0.66、0.56～1.21 mg·g^–1；多酚类成分总量为4.47～18.15 mg·g^–¹，酚酸类成分(新绿原酸、绿原酸、3-O-对香豆酰基奎宁酸)总量为1.94～14.14 mg·g^–1，黄酮类成分(异荭草素、荭草素、异牡荆素、牡荆素)总量为2.53～4.41 mg·g^–1。熵权-TOPSIS分析结果显示，80 ℃烘干较适合三叶青叶片干燥。该研究建立的方法准确、稳定、可靠；三种干燥方式对三叶青叶片多酚类成分含量影响显著，80 ℃烘干可能为最佳干燥方式，为后续三叶青叶片趁鲜加工工艺研究提供参考。

关键词: 三叶青, 超高液相色谱, 干燥方式, 熵权-TOPSIS法, 含量测定

CLC Number:

R932

LIN Mian-fa, FU Deng-ke, YANG Nan, HONG Yu-yan, PAN Meng-ting, FAN Shi-ming, XU Wen, XU Hui-long. Effects of Different Drying Methods on the Contents of 7 Polyphenols in Tetrastigma hemsleyanum Leaves[J]. Subtropical Plant Science, 2025, 54(6): 621-628.

林绵发, 付登科, 杨楠, 洪玉燕, 潘梦婷, 范世明, 许文, 徐惠龙. 不同干燥方式对三叶青叶片7种多酚类成分含量的影响[J]. 亚热带植物科学, 2025, 54(6): 621-628.

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Effects of Different Drying Methods on the Contents of 7 Polyphenols in Tetrastigma hemsleyanum Leaves

不同干燥方式对三叶青叶片7种多酚类成分含量的影响

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