Application and Research Progress of ITS2 Sequence in Plant Identification Using DNA Barcoding

doi:10.3969/j.issn.1009-7791.2014.04.017

›› 2014, Vol. 43 ›› Issue (04): 339-342.DOI: 10.3969/j.issn.1009-7791.2014.04.017

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Application and Research Progress of ITS2 Sequence in Plant Identification Using DNA Barcoding

FEI Xi-tong,JU Miao-miao,LIN Yuan,LI Bin,LI De-long,XIN Pei-yao

College of Forestry, Southwest Forestry University, Kunming 650224, Yunnan China

Received:2014-09-05 Revised:2015-01-26 Online:2014-12-10 Published:2014-12-10
Contact: XIN Pei-yao

ITS2序列在植物DNA条形码鉴定中的应用(综述)

费希同,巨苗苗,林源,李斌,李德龙,辛培尧

西南林业大学林学院，云南昆明 650224

通讯作者: 辛培尧
作者简介:费希同，硕士研究生，从事林木遗传育种研究。
基金资助:
云南省教育厅科学研究基金重点项目( 2012Z066)资助

Abstract

Abstract: The rapid development of DNA barcoding technology has greatly promoted the work of plant identification. With the increase of identification work and newly discovered sequences, the technology of ITS2 sequence has become a widely used identification at present. In order to provide certain theoretical reference of plant identification, this study introduced the ITS2 sequence identification process and analyzed the characteristics and problems occurred in the process, based on the characteristics of ITS2 sequence.

Key words: ITS2 sequence, plant identification, DNA barcoding, research progress

摘要： DNA条形码技术的迅速发展极大地推动了植物的鉴定工作，随着鉴定工作的不断进行和新序列的不断发现，利用ITS2序列进行鉴定已成为目前较为广泛使用的鉴定技术。本文根据ITS2序列的特点和性质，介绍ITS2序列鉴定的一般过程，并分析其特点和存在问题，以期为植物鉴定方面的研究提供参考。

关键词: ITS2序列, 植物鉴定, DNA条形码, 研究进展

CLC Number:

Q943.2

FEI Xi-tong,JU Miao-miao,LIN Yuan,LI Bin,LI De-long,XIN Pei-yao. Application and Research Progress of ITS2 Sequence in Plant Identification Using DNA Barcoding[J]. , 2014, 43(04): 339-342.

费希同,巨苗苗,林源,李斌,李德龙,辛培尧. ITS2序列在植物DNA条形码鉴定中的应用(综述)[J]. , 2014, 43(04): 339-342.

References

[1] 陈士林,姚辉,宋经元,李西文. 基于DNA barcoding(条形码)技术的中药材鉴定[J]. 世界科学技术-中医药现代化, 2007,9(3): 7—12.
[2] Li D Z, Liu J Q, Chen Z D, Wang H, Ge X J, Zhou S L, Gao L M, Fu C X, Chen S L. Plant DNA barcoding in China[J]. Journal of Systematics and Evolution, 2011,49: 165—168.
[3] Li M, Cao H, But P P H, Shaw P C. Identification of herbal medicinal materials using DNA barcodes[J]. Journal of Systematics and Evolution, 2011,49(3): 271—283.
[4] 陈士林. 中药DNA条形码分子鉴定[M]. 北京: 人民卫生出版社, 2012: 4.
[5] 李隆云,钟国跃,卫莹芳,秦松云. DNA分子标记及其在中药中的应用[J]. 中国中医药科技, 2002,9(5): 315—319.
[6] 王建波,张文驹,陈家宽. 核rDNA的ITS序列在被子植物系统与进化研究中的应用[J]. 植物分类学报, 1999,37(4): 407—416.
[7] Baldwin B G, Sanderson M J, Porter J M,Wojciechowski M F, Campbell C S, Donoghue M J. The ITS region of nuclear ribosomal DNA: Avaluable source of evidence on angiosperm phylogeny[J]. Annals of the Missouri Botanical Garden, 1995,82(2): 247—277.
[8] Spencer V M. Examining rates and patterns of nucleotide substitution in plants[J]. Plant Molecular Biology, 2000,42(1): 25.
[9] CBOL Plant Working Group. A DNA barcode for land plants[J]. Proceedings of the National Academy of Sciences of the United States of America, 2009,106(31): 12794.
[10] Chen S L, Yao H, Han J P, Liu C, Song J Y, Shi L C, Zhu Y J, Ma X Y, Gao T, Pang X H, Luo K, Li Y, Li X W, Jia X C, Liu Y L, Leon C. Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plants species[J]. PLoS ONE, 2010,5(1): e8613.
[11] 陈士林,宋经元,姚辉,石林春,罗焜,韩建萍. 药用植物DNA条形码鉴定策略及关键技术分析[J]. 中国天然药物, 2009,7(5): 322—327.
[12] 陈士林,庞晓慧,姚辉. 中药DNA条形码鉴定体系及研究方向[J]. 世界科学技术—中医药现代化, 2011,11(5): 747—754.
[13] Chiou S J, Yen J H, Fang C L, Chen H L, Lin T Y. Authentication of medicinal herb susing PCR-amplified ITS2 with specific primers[J]. Planta Medica, 2007,73: 1421—1426.
[14] Keller A, Schleicher T, Schultz J, Müller T, Dandekar T, Wolf M. 5.8S-28S rRNA interaction and HMM-based ITS2 annotation[J]. Gene, 2009,430: 50—57.
[15] 孙稚颖,罗红梅,陈士林. 大青叶及其混淆品的ITS2序列鉴定研究[J]. 世界科学技术—中医药现代化,2011,13(2):395—399.
[16] Hollingsworth P M. Refining the DNA barcode for land plants. PNAS, 2011,108(49): 19451—19452.
[17] Coleman A W. Pan-eukaryote ITS2 homologies revealed by RNA secondary structure[J]. Nucleic Acids Research, 2007,35(10): 3322—3329.
[18] Koch M A, Dobes C, Mitchell-Olds T. Multiple hybrid formation in natural populations: concerted evolution of the internal transcribed spacer of nuclear ribosomal DNA (ITS) in North American Arabis divaricarpa (Brassicaceae)[J]. Molecular Biology and Evolution, 2003,20(3): 338—350.
[19] 赵莎,辛天怡,侯典云, 庞晓慧,程睿旸,高建平. 党参药材及其混伪品的ITS/ ITS2条形码鉴定研究[J]. 世界科学技术-中医药现代化, 2013,15(3): 421—428.
[20] 罗焜,陈士林,陈科力,宋经元,姚辉,马新业, 朱英杰, 庞晓慧,余华,李西文,刘震. 基于芸香科的植物通用DNA条形码研究[J]. 中国科学: 生命科学, 2010,40(4): 342—351.
[21] 朱英杰,陈士林,姚辉,谭睿,宋经元,罗焜,鲁静. 重楼属药用植物DNA条形码鉴定研究[J]. 药学学报, 2010,45(3): 376—382.
[22] 李妮,陈科力,刘震,叶丛进,陈士林. 景天属药用植物DNA条形码研究[J]. 世界科学技术-中医药现代化, 2010,12(3): 463—467.
[23] 高婷,姚辉,马新业,朱英杰,宋经元. 中国黄芪属药用植物DNA条形码(ITS2)鉴定[J]. 世界科学技术-中医药现代化, 2010,12(2): 222—227.
[24] 石林春,陈士林,宋经元,姚辉,韩建萍. 豆蔻属药用植物DNA条形码鉴定研究[J]. 世界科学技术-中医药现代化, 2009,12(3): 1—6.
[25] 唐银琳,刘镛,姚辉,陈振东,吴耀生,徐鹏,蒋东. DNA条形码(ITS2)在葫芦科鉴定中应用价值的评估[J]. 植物科学学报, 2012,30(6): 631—638.
[26] 曾旭,李秋实,王钤,谢丽芳,张亚兰,罗焜,刘志华. 基于ITS2 条形码的五加皮基原植物及其易混品的鉴定[J]. 中国现代中药, 2012,14(2): 15—18.
[27] 石林春,宋经元,李莉,姚辉,陈士林. 基于ITS2条形码序列的京大戟及其易混品的DNA分子鉴定[J]. 环球中医药, 2011,4(2):103—108.
[28] 高婷,姚辉,陈士林. 基于ITS2序列的藁本与常见混伪品的分子鉴定[J]. 世界科学技术-中医药现代化, 2011,13(2): 418—423.
[29] 冯思玲. 系统发育树构建方法研究[J]. 信息技术, 2009(6): 38—44.
[30] 罗焜,马培,姚辉,宋经元. 基于ITS2序列鉴定川贝母及其混伪品基原植物[J]. 世界科学技术-中医药现代化, 2012,14(1): 1153—1158.
[31] 刘美子,刘萍,李美妮,姚辉. 党参及其易混伪品的ITS2分子鉴定[J]. 世界科学技术-中医药现代化, 2011,13(2): 412—417.
[32] 郑司浩,孙稚颖,黄林芳. 麦冬及其混伪品的ITS2序列分析及鉴别研究[J]. 中国现代中药, 2012,14(1): 21—23.
[33] 孙涛,孔德英,滕少娜,陆丽华,邓朝晖,李应国. 基于ITS2序列的黄连及其伪混品的分子鉴定[J]. 贵州农业科学, 2013,41(9): 20—22.

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Application and Research Progress of ITS2 Sequence in Plant Identification Using DNA Barcoding

ITS2序列在植物DNA条形码鉴定中的应用(综述)

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