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10 December 2014, Volume 43 Issue 04 Previous Issue    Next Issue
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Response of Carbon Amount Yield and Quantity of Different Nitrogen Use Efficiency Rape Cultivars to Different Nitrogen Conditions YU Jia-ling,SHU Jia-bin,LIAO Qiong,SONG Hai-xing,ZHANG Xiao-long,GUAN Chun-yun 2014, 43(04): 271-275.  DOI: 10.3969/j.issn.1009-7791.2014.04.001 Abstract ( )   PDF (215KB) ( )   In this study, a pot experiment was conducted to study the differences of organic carbon among rape leaf, silique, pod area and rapeseed, yield and rapeseed oil content in two rape cultivars [High nitrogen (N) use efficiency cultivar, No. 27; low N use efficiency cultivar, No. 6] under two N application levels for exploring the law of carbon accumulation. The results showed that No. 27 ranked higher level than No. 6 in rapeseed yield, harvest index, rapeseed oil content. The accumulation of organic carbon and dry matter in root and leaf of No. 27 ranked higher level than those of No. 6 during bolting, flowering and podding stages, while the results at harvest stage exhibited the contrary trend. Additionally, organic carbon accumulation and dry matter in leaf of No. 27 was lower than that of No. 6 under N-deficient condition at harvest stage, but the result was opposite under N-sufficient condition. The organic carbon and dry matter accumulation in flower, silique and rapeseed of No. 27 were higher than those of No. 6 at reproductive stage, while vegetative growth state exhibited the opposite results in rape stem. Meanwhile, the organic carbon and dry matter in stem of No. 27 accumulated remarkably at podding stage. The organic carbon accumulation of No. 27 was higher than that of No. 6 at early growth period, while the carbon and dry matter of reproductive organs and stem in No. 27 ranked higher level compared to No. 6 at later growth stage. The two varieties showed high rapeseed yield but low oil content under two N application levels, and dry matter accumulation of nutritive organs declined remarkably. The organic carbon accumulation was tightly correlated with rapeseed oil content. In total, carbon accumulation of No. 27 is stronger than that of No. 6 at early stage, meanwhile, organic carbon, dry mass at lately growth stage and stem transportation is also stronger. References | Related Articles | Metrics

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Effects of Five Kinds of Heavy Metal Stress on Seed Germination and Seedling Growth of Trifolium repens and T. pratense SONG Feng-ming,LIU Jian-hua,WU Cai-qiong,LEI Jiang-li,XU Yi-yan 2014, 43(04): 276-280.  DOI: Doi: 10.3969/j.issn.1009-7791.2014.04.002 Abstract ( )   PDF (216KB) ( )   Effects of five kinds of heavy metal stress on seed germination and seedling growth of Trifolium repens and T. pratense were studied by germination test, aimed to provide a reference for the study of the applications of Trifolium in heavy metal contaminated soil. The results showed that Haifa (a cultivar of T. repens)and T. pratense had better tolerance to Cd2+ and Mn2+, than to Pb2+, Cu2+ and Zn2+.They had over 80% germination rate under the III grade standard concentration of Cd2+ or under 800 mg稬-1 Mn2+, and the seedlings still maintained good vitality. High concentration of heavy metal ions stress had significant inhibition on the growth of embryo and hypocotyl, and T. pratense had better germination rate than Haifa. These two species of Trifolium could be applied for Cd-Mn contaminated soil restoration. References | Related Articles | Metrics

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Study on Photosynthetic Characteristics of Camellia fluviatilis YANG Ya-hui, HUANG Yong-fang 2014, 43(04): 281-285.  DOI: 10.3969/j.issn.1009-7791.2014.04.003 Abstract ( )   PDF (270KB) ( )   The photosynthetic characteristics of Camellia fluviatilis were studied by LI -6400 (Li-Cor Inc, USA) portable photosynthesis system. The results showed that the curves of diurnal variation in Pn (net photosynthetic rate) had a peak at about 13:00 pm, and reached to 18.65 μmol·m-2·s-1; light compensation point of photosynthesis of C. fluviatilis in un-folding leaves was 12 µmol·m-2·s-1, light saturation point of photosynthesis was 500 µmol·m-2·s-1. The correlation analysis showed that: (1) The Pn of C. fluviatilis had extremely significant positive correlation (P≤0.01) with light intensity and air temperature, the correlation coefficients were 0.715 and 0.939 respectively; (2) There was significant negative correlation between Pn of C. fluviatilis and intercellular CO2 concentration (P≤0.01), the correlation coefficient was -0.752; (3) The Pn of C. fluviatilis also had a negative correlation with the atmosphere CO2 concentration (P≤0.05), correlation coefficient of which was -0.605. References | Related Articles | Metrics

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Preliminary Study of Predominant Factors on Chromosome Doubling of Betula alnoides CHEN Rong,ZHU Chang-san,PANG Zheng-hong 2014, 43(04): 286-288.  DOI: 10.3969/j.issn.1009-7791.2014.04.004 Abstract ( )   PDF (182KB) ( )   In this paper, a method for chromosome doubling of Betula alnoides was developed. Terminal buds from aseptic germination of B. alnoides were used as explants, effect factors on chromosome doubling of B. alnoides were discussed, such as concentration of colchicine, co-culture time of colchicine, preculture time. The results showed that the concentration of colchicine was better to be 120 mg稬-1 for chromosome doubling of B. alnoides, and maximum chimaera number would be obtained in the regermination plantlet of B. alnoides, after preculture without colchicine for 10 d and then co-culture with colchicine for 15 d. References | Related Articles | Metrics

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Induction of Callus of Borneol Camphor Caulicle XU Jian,LI Hui-hua,CHANG Qiang,WANG Wei,SU Ming-hua 2014, 43(04): 289-292.  DOI: 10.3969/j.issn.1009-7791.2014.04.005 Abstract ( )   PDF (311KB) ( )   In this study, caulicle of borneol camphor was used as culture material, the optimal medium with different combination of growth regulator was screened using orthogonal method. The time of callus induction, the growth state, fresh weight growth index and D-borneol content of callus were measured. The results showed that the optimal growth regulator combination was 2 mg·L-1 2,4-D and 0.6 mg·L-1 6-BA, and extract yield of D-borneol was 2.6698 μg·g -1 DW. References | Related Articles | Metrics

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Optimization of Extracting Technology of Total Flavonoids in Phyllanthus emblica by Ultrasonic-assisted CHEN Hong-bin,ZHOU Jie,WU Jin-hui,CAI Ying-qing,ZHANG Miao-xia 2014, 43(04): 293-297.  DOI: 10.3969/j.issn.1009-7791.2014.04.006 Abstract ( )   PDF (383KB) ( )   The emblica flavonoid was extracted from dried emblica powders by ultrasonic-assisted extraction, four factors (liquid-material ratio, ethanol concentration, ultrasonic times and ultrasonic powers) were investigated by the single factor experiments. The process parameters were optimized by response surface analysis method. The optimum extraction conditions of Phyllanthus emblica total flavonoids were determined as: liquid-material ratio 82:1 (V/W), ethanol concentration 45%, ultrasonic times 41 min and ultrasonic powers 210 W. Under this condition, the extraction of total flavonoids was 6.12%. References | Related Articles | Metrics

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Determination of Pb, Cu and Cd in Anoectochilus roxburghii with Wet Digestion and Graphite Furnace Atomic Absorption Spectrometry WANG Wei,CAI Xiao-dong,LI Hui-hua,CHANG Qiang,SU Ming-hua 2014, 43(04): 298-301.  DOI: 10.3969/j.issn.1009-7791.2014.04.007 Abstract ( )   PDF (246KB) ( )   The samples were treated by wet digestion method to measure the content of Pb, Cu, Cd in Anoectochilus roxburghii by using graphite furnace atomic absorption spectrometry (GFAAS). The results showed that the atomization temperature of Pb, Cu, Cd were 2100, 2200 and 1800 ℃, the ashing temperature were 400, 800 and 250 ℃, and their detection limits were 0.47, 0.50 and 0.62 μg·L-1, respectively. The spike recoveries ranged from 92.7% to 94.0%, and the average precision relative standard deviation was 1.71%, which showed that this method is effective and reliable for determination of Pb, Cu, Cd in A. roxburghii. References | Related Articles | Metrics

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Preparation Process and Anti-viral Activities of Agaropectin from Gracilaria tenuistipitata BAN Zhen,LIN Yi 2014, 43(04): 302-304.  DOI: 10.3969/j.issn.1009-7791.2014.04.008 Abstract ( )   PDF (276KB) ( )   The 0.46 g agaropectin was prepared from 20.0 g agar by using DEAE-cellulose. The agaropectin sample showed not any activities against Escherichia coli and Staphylococcus aureus. However, obvious activity against tobacco mosaic virus (TMV) was observed to the agaropectin sample with a concentration-dependent manner, 37.02%—81.51% inhibition rate corresponding to 0.05—2.0 mg·mL-1 agaropectin. The results presented would provide a base for further exploration of agaropectin, which was the by-product of agarose preparation. References | Related Articles | Metrics

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Analysis of the Characteristics of Ancient Trees Resource in Qiannan Prefecture of Guizhou Province TIAN Hua-lin,FU Yong-li,XIANG Xia,DU Hong-ye 2014, 43(04): 305-309.  DOI: 10.3969/j.issn.1009-7791.2014.04.009 Abstract ( )   PDF (265KB) ( )   The quantity distribution, species composition and floristic elements of 5571 ancient trees consisted of 56 families, 122 genera and 213 species in Qiannan Prefecture were analyzed. The results showed that ancient trees resource in Qiannan Prefecture was almost of third-class ancient trees, accounted for 79.7% of total trees, the diameter was mostly below 2 m, maximum diameter was 4.9 m. Ancient trees were rarely in towns, courtyard, rural houses and temples. There were 95.7% of the ancient trees distributed in the countryside, and most of them were scattered distribution. The dominant families with species composition were Fagaceae, Lauraceae, Magnoliaceae, Ulmaceae and Pinaceae; the dominant families with number composition were Hamamelidacea, Ulmaceae, Cupressaceae, Lauraceae and Ginkgoacea; the dominant species were Liquidambar formosona, Cupressus funebris, Zelkova schneideriana, Cinnamomum camphora and Ginkgo biloba. There were more relict species, endemic species and Chinese protection species. Areal types and subtypes of families, genera, species were various, the flora of families and species was mainly Tropic, but at the level of genera, there was no significant difference between temperate element and tropical element; tropical element of families was tend to Pantropic, tropical element of genus and species were Pantropic and Tropic Asia; temperate element of families was North Temperate and South Temperate disjunct distribution, temperate element of genera was North Temperate, East Asia, North America disjuncted and East Asia distribution, and temperate element of species was North Temperate. References | Related Articles | Metrics

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Resources of the Vascular Plants in Lagou Nature Reserve of Guangxi LUO Kai-wen,PENG Ding-ren,QIN Yong-hua,ZHANG Xian-lai 2014, 43(04): 310-313.  DOI: 10.3969/j.issn.1009-7791.2014.04.010 Abstract ( )   PDF (282KB) ( )   Based on plant investigation of Lagou Nature Reserve in Guangxi, the result indicated that the vascular plants are bundant, represented by 1078 species (including varieties, subspecies), belonging to 657 genera in 186 families. Among these plants found, there are about 88 timber plants, 358 medicinal plants, 36 oil and fatty plants, 70 fiber plants, 29 starch plants, 25 fruit plants, 28 aromatic plants, 39 tannic plants, 12 drinking plants, 49 forage plants, 75 ornamental plants, 23 soil-conserving plants, 43 rare and endangered plants. It’s important to protect the plant resources, and suggestions about conservation and sustainable utilization of the resources were put forward. References | Related Articles | Metrics

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Effect of Different Factors on the Growth of Piper kadsura under Forest KANG Yong-wu 2014, 43(04): 314-317.  DOI: 10.3969/j.issn.1009-7791.2014.04.011 Abstract ( )   PDF (187KB) ( )   The L9 (34) orthogonal design was applied to test the effects of afforestation method, slope position and canopy density with 3 levels for each treatment on the growth of Piper kadsura seedling under forest. The results showed that: afforestation method, slope position and canopy density have significant influence on biomass fresh weight, the total vine length and the number of primary branches; afforestation method has a significant on the main stem base diameter; primary and secondary factors were afforestation method, canopy density and slope position on promoting biomass fresh weight, the total vine length and the number of primary branches; the optimal combination of promoting biomass fresh weight, the total length of vine, the number of primary branches and the main stem base diameter of P. kadsura are light matrix container seedling planting, downslope, canopy density 0.5—0.7. References | Related Articles | Metrics

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Interplanting Camellia oleifera under Deciduous forest of Huanghua Pear YU Hong-ying 2014, 43(04): 318-320.  DOI: 10.3969/j.issn.1009-7791.2014.04.012 Abstract ( )   PDF (221KB) ( )   Different interplanting methods (inter-plant, inter-row and intersection) using 2 a Camellia oleifera grafted seedling interplanted with 15 a Huanghua pear tree were carried out, then the growth and coning quantity of 6 a plants of C. oleifera, the production and quality of Huanghua pear were investigated. The results showed that: intersection planting was best for oil-tea, average crown breadth was upto 141.0 cm, the average survival rate was 91.5%, and the average yield of C. oleifera seeds was 3.86 kg per plant; there was little effect on production of chrysanthemum pear by intersection and inter-row planting; while, inter-planting had great effect on production of chrysanthemum pear, the production per plant and high grade fruit rate of chrysanthemum pear were lower 20.4% and 23.3% than control, respectively. References | Related Articles | Metrics

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Investigation and Control Measures of Harmful Plants in Urban Green Land of Guangzhou City DONG Bin,XIE Xiu-feng,ZHANG Xiang-hui,LIANG Chun-hui,CHEN Li-fen 2014, 43(04): 321-325.  DOI: 10.3969/j.issn.1009-7791.2014.04.013 Abstract ( )   PDF (318KB) ( )   Based on a survey on various types of urban green lands which were seriously invaded, the article listed harmful plants which爓ere seriously damaged in urban green land of Guangzhou. Moreover, characteristics, ways爋f invasion and areas爋f invasion of harmful plants were concluded. All of the results revealed the rules of harmful plants intruded into the urban green land of Guangzhou, or even Guangdong, the protect and control measures were proposed so as to provide relevant information for monitoring and management. References | Related Articles | Metrics

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The Introduction of Cassia bakeriana and Its Landscape Application CHEN Heng-bin 2014, 43(04): 326-326.  DOI: 10.3969/j.issn.1009-7791.2014.04.014 Abstract ( )   PDF (233KB) ( )   The morphological characteristics of Cassia bakeriana introduced recently in Xiamen were described. The methods of propagation and techniques of cultivation were reported. Meanwhile, the application in landscape was also recommended. Related Articles | Metrics

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Rapid Propagation of the Hybrid Seeds of Den. nobile♀ ×Den. moniliforme♂ and Flowering in vitro ZHUO Xiao-kang,CHEN Yan-qiong,LI Shu-xian,QI Zi-yu,PENG Dong-hui,LAN Si-ren 2014, 43(04): 327-328.  DOI: 10.3969/j.issn.1009-7791.2014.04.015 Abstract ( )   PDF (286KB) ( )   he F1 hybrid seeds of Den. nobile♀ × Den. moniliforme♂ were taken as materials to explore in vitro rapid propagation techniques and flowering. The results showed that the optimum media for seedlings germination was 1/2MS + 6-BA 1.0 mg·L-1 + NAA 2.0 mg·L-1+ IBA 2.0 mg·L-1+ banana mud 100 g·L-1. The optimum medium for proliferation of subculture was 1/2MS + 6-BA 0.2 mg·L-1+ NAA 1.0 mg·L-1 + banana mud 100 g·L-1 with the proliferation coefficient of 4—5 times. The medium for regeneration of adventitious buds was 1/2MS + 6-BA 2.0 mg·L-1+ NAA 0.2 mg·L-1+ peptone 2 g·L-1 with 93.3% induction rate and 3.7 times induction coefficient. The rooting medium was TH + 6-BA 0.5 mg·L-1+ NAA 1.0 mg·L-1, the rooting rate was up to 91.7% with 3—5 roots. The medium for the floral bud induction was 6-BA 2.0 mg·L-1 + NAA 0.5 mg·L-1 + IBA 0.5 mg·L-1 with the rate of 8%. References | Related Articles | Metrics

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Overview of Cold Resistance in Woody Plants GUO Xue-min,LIU Jian-zhen,LIU Yong-jun,XIAO Xiao,GAO Rong-fu 2014, 43(04): 329-338.  DOI: 10.3969/j.issn.1009-7791.2014.04.016 Abstract ( )   PDF (329KB) ( )   Cold resistance or freezing tolerance is used to represent the ability of plants to adapt to and withstand freezing temperatures. It is a complex, multigenetic trait, and its research has a wealth of history. Recent studies provide an important clue for plants responding to low temperature and acquiring cold resistance. In this paper, advances in ice nuclation and its transmission, deep supercooling and vitrification, physiology and genetic regulation of cold acclimation, molecular biology of cold acclimation and genetic engineering approaches were summarized in woody plants, which provide references for improving cold hardiness in economically important crops while still maintaining high yield. References | Related Articles | Metrics

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Application and Research Progress of ITS2 Sequence in Plant Identification Using DNA Barcoding FEI Xi-tong,JU Miao-miao,LIN Yuan,LI Bin,LI De-long,XIN Pei-yao 2014, 43(04): 339-342.  DOI: 10.3969/j.issn.1009-7791.2014.04.017 Abstract ( )   PDF (263KB) ( )   The rapid development of DNA barcoding technology has greatly promoted the work of plant identification. With the increase of identification work and newly discovered sequences, the technology of ITS2 sequence has become a widely used identification at present. In order to provide certain theoretical reference of plant identification, this study introduced the ITS2 sequence identification process and analyzed the characteristics and problems occurred in the process, based on the characteristics of ITS2 sequence. References | Related Articles | Metrics

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Research Advances on Rapid Propagation and Germplasm in Toona sinensis XIAO Hua-shan,XIAO Xiang-xi,HE Wen-guang,LIN Xin-chun,ZHENG Yang-ling 2014, 43(04): 343-346.  DOI: 10.3969/j.issn.1009-7791.2014.04.018 Abstract ( )   PDF (279KB) ( )   Toona sinensis, grows rapidly, distributes widely in Southeast Asia and all over the country in China. Applications in wide-area for mankind were known, for instance afforestation, wood, medicinal and edible. This paper reviewed the research advances on rapid propagation and germplasm of T. sinensis in recent years. The objective was to provide data of micropropagation, breeding and utilization in T. sinensis. References | Related Articles | Metrics

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Subtropical Plant Science Contents (2013—2014) 2014, 43(04): 347-356.  Abstract ( )   PDF (353KB) ( )   Related Articles | Metrics