Subtropical Plant Science ›› 2016, Vol. 45 ›› Issue (01): 53-56.DOI: 10.3969/j.issn.1009-7791.2016.01.011

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A Method for Extraction of Total RNA from Cinnamomum kanehirae

MENG Hong-yan,GUO Ying,LIN Wen-zhen,WANG Wen-hua,LIU Li-qing   

  1. (Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant,燜ujian Institute of Subtropical Botany, Xiamen 361006, Fujian China)
  • Received:2016-01-15 Revised:2016-04-17 Online:2016-03-10 Published:2016-03-10
  • Contact: GUO Ying

台湾牛樟总RNA提取方法的建立

孟红岩,郭莺,林文珍,汪文华,刘黎卿   

  1. (福建省亚热带植物研究所,福建省亚热带植物生理生化重点实验室,福建 厦门 361006)
  • 通讯作者: 郭莺
  • 作者简介:孟红岩,博士,助理研究员,从事植物分子生物学研究。
  • 基金资助:

    厦门市对台科技合作项目(3502Z20131150)

Abstract: In this study, total RNA extraction of roots, stems, and leaves from Cinnamomum kanehirae was carried out. Electrophoresis, Nanodrop and Aglient 2100 analysis indicated that the value of 28S/18S was 1.7 to 2.1, RNA?integrity number was higher than 7, and RNA concentration was higher than 50 ng·μL-1, indicating that RNA was intact. In summary, the study showed that the purity, concentration and?integrity of the isolated RNA were qualified, and could be used for subsequent molecular biology studies.

Key words: Cinnamomum kanehirae, RNA extraction

摘要: 以牛樟Cinnamomum kanehirae根、茎、叶为材料,在RNAprep Pure Plant Kit (Polysaccharides & Polyphenolics-rich)方法的基础上进行改良,建立台湾牛樟总RNA的提取方法。经琼脂糖凝胶电泳、Nanodrop和Aglient 2100检测发现,牛樟根、茎、叶所得RNA的28S和18S比值介于1.7~2.1之间,RNA完整性较好,浓度均在50 ng·μL-1以上,RIN值均大于7。该方法提取的RNA纯度、浓度和完整性均合格,可满足后续分子生物学实验要求。

关键词: 牛樟, RNA提取

CLC Number: