Abstract: Genome DNA of Hainan Oryza meyeriana with high resistance and vigorous growth was extracted, then was transformed into rice restorer R225, R128, R15 by stem injecting.From F₁ generation, variation rice from others in growth period, height, type and spikelets was chosen.After 3 generation, 15 stable rice materials were screened out.The materials and controls were detected with SSR markers, which indicated that there were 4 materials from which DNA fragments specific to wild rice were amplified.The result showed that variation materials from Oryza meyeriana were acquired.

Key words: Hainan, Oryza meyeriana, genome DNA, transformation, SSR detection

摘要： 选取抗性较好且生长旺盛的海南疣粒野生稻,提取其基因组DNA,通过穗颈注射法将其导入杂交水稻恢复系R225、R128、R15。第1代开始选择在生育期、株高、株叶型和穗粒结构等性状与群体中其他单株有明显不同的变异株,经过3代选择,筛选到15份稳定材料。利用SSR分子标记检测4份导入后代材料具有供体而受体无的DNA条带,说明本试验获得了来自疣粒野生稻的变异材料。

关键词: 海南, 疣粒野生稻, 基因组DNA, 导入, SSR检测