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Subtropical Plant Science

Subtropical Plant Science ›› 2009, Vol. 38 ›› Issue (01): 12-14,18.

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Effects of Nitrogen Application and Cotyledon Excision on Leaf Nitrate Reductase Activity of Swietenia macrophylla Seedling

YAN Xing-fu1, TIAN Wei-min2   

  1. 1. College of Life Science and Engineering, Northern University for Nationalities, Yinchuan 750021, Ningxia China;
    2. Rubber Research Institute, Chinese Academy of Tropical Agricultural Science, Danzhou 571737, Hainan China
  • Received:2008-09-20 Online:2009-03-10 Published:2009-03-10

施氮和去除子叶对大叶桃花心木幼苗叶片硝酸还原酶活性的影响

闫兴富1,田维敏2   

  1. 1. 北方民族大学 生命科学与工程学院, 宁夏 银川 750021;
    2. 中国热带农业科学院 橡胶研究所, 海南 儋州 571737
  • 作者简介:闫兴富(1968-),男,河南周口人,副教授,博士,从事种子生物学和植物生理生态学研究。
  • 基金资助:

    国家自然科学基金(No.30460107);北方民族大学博士科研启动基金资助

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Abstract: Under nitrogen application and cotyledon excision,the nitrate reductase activity (NRA) of seedling leaf of Swietenia macrophylla,a tropical defoliated arbor of Meliaceae,was studied.The results showed that the leaf NRA increased first but decreased then with the development of leaves under non-nitrogen application.After nitrogen application,the leaf NRA of seedlings of all sampling periods (except 35 days after transplanting) were significantly higher (P<0.05) than that of the control and a gradual decrease was observed during the development period of leaves.The leaf NRA of S. macrophylla seedling increased significantly (P<0.05) after 4 weeks of cotyledon excision during different development periods.

Key words: Swietenia macrophylla, nitrogen application, cotyledon excision, NRA

摘要： 研究了热带落叶乔木大叶桃花心木(Swietenia macrophylla)在施氮和去除子叶后幼苗叶片的硝酸还原酶活性(NRA)变化。结果表明,在非施氮(对照)条件下,NRA随着幼苗叶片的发育先升高后降低;施氮后幼苗叶片NRA在各取样时期(除35 d外)均显著高于非施氮处理(P<0.05),并随着取样时期的延续,叶片NRA逐渐降低。在幼苗发育的不同时期去除子叶,4周后,叶片NRA均显著升高(P<0.05)。

关键词: 大叶桃花心木, 施氮, 去除子叶, 硝酸还原酶活性

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