Subtropical Plant Science ›› 2009, Vol. 38 ›› Issue (02): 19-21,25.DOI: 10.3969/j.issn.1009-7791.2009.02.005
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GAO Li, YANG Bo, LI Hong-lin
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高丽,杨波,李洪林
作者简介:
基金资助:
中国科学院知识创新工程重要方向性项目(KSCX2-YW-N-032)资助
Abstract: Stems of tissue culture seedlings of Ormosia henryi were cultured under low temperature stress. The results showed that the optimal temperature for induction of chilling tolerance callus was 5℃. The differentiation frequency of chilling tolerance callus cultured under 5℃ was 70.0% on MS solid medium supplemented with 6-BA 2.0 mg/L and NAA 0.25 mg/L, and the injury rate of regenerated plants from chilling tolerant callus was much lower than that of control group.
Key words: Ormosia henryi, low temperature stress, chilling tolerance
摘要： 以花榈木组培苗茎段为试材,在低温胁迫条件下进行耐冷性愈伤组织诱导培养。结果表明,5℃是花榈木茎段低温胁迫培养及耐冷性诱导的最适温度。经过5℃低温胁迫培养获得的耐冷性愈伤组织在分化培养基MS+6-BA 2.0 mg/L+NAA 0.25 mg/L上的分化率达70.0%,且再生植株的低温伤害率明显低于对照。
关键词: 花榈木, 低温胁迫, 耐冷性
CLC Number:
Q945.78
GAO Li, YANG Bo, LI Hong-lin. Stems Culture under Low Temperature Stress and Chilling Tolerance Induction of Ormosia henryi Seedlings Cultured in vitro[J]. Subtropical Plant Science, 2009, 38(02): 19-21,25.
高丽,杨波,李洪林. 花榈木组培苗茎段低温胁迫培养及耐冷性诱导[J]. 亚热带植物科学, 2009, 38(02): 19-21,25.
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