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30 April 2021, Volume 50 Issue 02 Previous Issue    Next Issue
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Plant physiology and biochemistry
Plant ecology, resources and taxonomy
Plant protection
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Plant physiology and biochemistry

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Isolation of Nobiletin from Citrus depressa Peel and Its Anti-tumor Activity GU Yue, LIU Shao-song, HE Li-juan, SHEN Rui-chi, HUANG Jun-mei 2021, 50(02): 83-87.  DOI: 10.3969/j.issn.1009-7791.2021.02.001 Abstract ( )   PDF (370KB) ( )   Using Citrus depressa peel as the raw meterial, we extracted nobiletin by ultrasound and identified it with high performance liquid chromatography, then explored the anti-tumor activity of nobiletin. The effect of nobiletin on the proliferation was studied through MTT assay in lung carcinomas, hepatocellular carcinoma and cervical carcinomas cells. Flow cytometry was used to detect the apoptosis of hepatocellular carcinoma cells by double staining. The results showed that nobiletin could inhibit the growth of cancer cells in a broad spectrum and promote the apoptosis of hepatocellular carcinoma cells. References | Related Articles | Metrics

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Fruit Nutritional Evaluation of Different Phyllanthus emblica Varieties WEI Wen-hua, TAN Shi-jun, ZHOU Wei-chao, XU Zhu-liang 2021, 50(02): 88-91.  DOI: 10.3969/j.issn.1009-7791.2021.02.002 Abstract ( )   PDF (245KB) ( )   In order to systematically analyze and evaluate fruit nutritional qualities of different Phyllanthus emblica varieties, ‘Chipi’, ‘Boliyougan’, ‘Dongkengtian’, ‘Bingtian’, ‘Liuyuebai’ and ‘Matitian’ were used as materials. Seven nutrient contents were analyzed and evaluated by the principal component analysis method. The results showed that the contents of vitamin C and reducing sugars in each cultivar were relatively high; Vitamin C had a significant positive correlation with protein, and reducing sugar had a significant positive correlation with soluble solids. Three principal components were extracted through principal component analysis. Two varieties, ‘Chipi’ and ‘Boliyougan’, with higher comprehensive nutrients were screened out as excellent varieties for the construction of P. emblica high-yield forests. References | Related Articles | Metrics

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Antibacterial and Antioxidant Activities of Citronella Essential Oil YANG Hui-xiang, HUANG Xiao-qin, PAN Lei, SHI Yu, YE Bin-ji, DING Hong-yang, LI Yong-yu 2021, 50(02): 92-95.  DOI: 10.3969/j.issn.1009-7791.2021.02.003 Abstract ( )   PDF (258KB) ( )   This paper chose citronella which distributed in Xiamen of Fujian Province as materials. The citronella essential oil was extracted to against six pathogens (Pseudomonas spp., Serratia marcescens, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Chromobacterium violaceum) for antibacterial activity, and the antioxidant activity of citronella essential oil was studiedABTS.+ free radical scavenging and superoxide anion scavenging. The results showed that the essential oil extraction yield of citronella was 0.29, also affirmed that the oil had inhibitory effect on all of the tested strains and the inhibitory activity to Chromobacterium violaceum was the strongest. Citronella essential oil had high capacity to remove the ABTS.+ and superoxide anion. References | Related Articles | Metrics

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Dynamic Accumulation and Determination of Triptolide in Different Growth Period of Tripterygium wilfordii YUAN Yu-hong, SUN Yan, ZHENG Yu-shan 2021, 50(02): 96-100.  DOI: 10.3969/j.issn.1009-7791.2021.02.004 Abstract ( )   PDF (296KB) ( )   To explore the dynamic accumulation of triptolide in different growth period of Tripterygium wilfordii, HPLC method was used to determine triptolide in different parts and different harvesting periods in T. wilfordii. The results showed that the linear relationship of triptolide in 7.75～250 μg·mL-1 was good (R2=0.9997), the recovery of T. wilfordii was 99.21% with RSD 1.578%. This method had a good recovery rates, the content of triptolide in the leaves of T. wilfordii in the whole harvest periods was slightly higher than that in roots, which had the potential for resource development and utilization. T. wilfordii had the highest triptolide content in the leaves during July to August. From November to December, T. wilfordii had the highest triptolide content in the roots. This study provided a certain scientific basis for the artificial harvesting, quality evaluation and resource development of T. wilfordii. References | Related Articles | Metrics

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Biochemical Characteristics of Camellia oleifera Cutting in Rooting Process YE Xiao-ping, HUANG Yong-fang, TAN Sha, LI Meng-nan, DAI Qiu-yue 2021, 50(02): 101-105.  DOI: 10.3969/j.issn.1009-7791.2021.02.005 Abstract ( )   PDF (216KB) ( )   In the cuttings propagation experiment, the semi-lignified branches of 4-year-old Camellia oleifera ‘Cenruan 2’ were treated with different hormone types, the changes of peroxidase activity, soluble protein and chlorophyll in the cutting woods rooting process were studied, and the relationship between cutting woods and biochemical indices was discussed. The results showed that the activities of peroxidase, soluble protein and chlorophyll were significant difference in different days after softwood cutting (P<0.01). POD activities showed double peak curve of ‘rise, fall, rise sharply and down’, while soluble protein showed curve of ‘rise, fall, rise’, chlorophyll showed curve of ‘fall, rise, down and rise’. Compared to the control, the pear of curve of peroxidase, soluble protein and chlorophyll obviously increased. It was concluded that hormone directly affected the rooting ability of Camellia oleifera cuttings. References | Related Articles | Metrics

Plant ecology, resources and taxonomy

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Textual Research on Scutellaria planipes in Liaoning, China and Its Morphological Differences from S. pekinensis LI Ding-nan, WANG Xin, ZHANG Shu-mei 2021, 50(02): 106-110.  DOI: 10.3969/j.issn.1009-7791.2021.02.006 Abstract ( )   PDF (335KB) ( )   Scutellaria planipes Nakai et Kitag., a perennial herb of genus Scutellaria L., has only been found in Lingyuan City, Liaoning Province and is classified as a third-class protected plant in Liaoning province. This species was first published in 1934, however, Flora Reipublicae Popularis Sinicae, Flora of China and Species Catalogue of China all identified it as a synonymy of Scutellaria pekinensis Maxim.. In this study, a textual research on protologue, holotype, type locality, historical and current distribution area of S. planipes as well as the comparative study on morphology and pollen grains between the two species confirmed that S. planipes is an independent species and an additional description of the fruit was also made. The results here provide a reliable basis for the identification, conservation and further research on medicinal value of S. planipes. References | Related Articles | Metrics

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Growth Traits of Eucalyptus urophylla Under Different Water and Fertilizer Conditions YANG Hui-xiao, XU Fang, YANG Xiao-hui, LIAO Huan-qin, ZHANG Wei-hua, PAN Wen 2021, 50(02): 111-117.  DOI: 10.3969/j.issn.1009-7791.2021.02.007 Abstract ( )   PDF (283KB) ( )   In order to reveal the response mechanism of growth and bark thickness for Eucalyptus urophylla under different water and fertilizer gradients, 31 clones treated with nine combination of water and nutrients were used as experimental materials. After 18 months of artificial control of water and nutrient, the height, ground diameter, height under branch, 1/4 diameter, 2/4 diameter, 3/4 diameter and bark thickness were measured. The co-efficient of variation, Duncan multiple comparison and principal components were analyzed, and excellent clones were selected. The results showed that the growth and bark thickness traits of E. urophylla clones had great variation. Among them, the variation coefficient of height under branch and ground diameter was 50.78% and 12.46%, respectively, which suggested that analyzed traits had extensive variation with rich genetic diversity. There were significant differences in height, ground diameter, bark thickness, 1/4 diameter, 2/4 diameter and 3/4 diameter of E. urophylla clones under different water and fertilizer treatments. All clones performed better under 32 and 33 treatments. The 6 traits could be integrated into 3 principal components, and the cumulative contribution rate of the first 3 principal components was 84%. According to the genotype values of height, bark thickness and ground diameter, the best clones were 30, 7, 1, 29, 20 and 6. The average genetic gain was increased by 10.73 cm, 0.02 mm and 0.83 mm, reaching 5.29%, 6.38% and 2.24%, respectively. References | Related Articles | Metrics

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Effect of Shading on Adaptive Phenotype of Color-leafed Heuchera micrantha in Summer ZHOU Jia, SONG Li-li 2021, 50(02): 118-124.  DOI: 10.3969/j.issn.1009-7791.2021.02.008 Abstract ( )   PDF (296KB) ( )   Three Heuchera micrantha cultivars, ‘Georgia Peach’, ‘Petite pearl fairy’, ‘Tapestry’, were used to investigate the influence of shading on ornamental characteristics, semi-lethal high temperature and survival rate. Results showed that shading resulted in increase of the height, leaf area and inflorescence length, but delay of flowering of the three cultivars to varying degrees. The flowering period of ‘Georgia Peach’ and ‘Petite pearl fairy’ lengthened while that of ‘Tapestry’ shortened in response to shading treatment. With the increase of shading degree, the contents of chlorophyll and carotenoid in ‘Georgia Peach’ and ‘Petite pearl fairy’ increased significantly but the anthocyanin content declined evidently. Accordingly, the leaf color turned from red or purple to grey and green. In a different way, the contents of chlorophyll, carotenoid and anthocyanin in ‘Tapestry’ rose obviously under shading condition and the leaf developed into dark green with crimson leaf vein. The semi-lethal high temperature and survival rate of the three cultivars increased remarkably with the promotion of shading degree. Correlation analysis revealed that there were extremely significant negative correlations between survival rate and extreme high temperature, extreme high light intensity, air temperature and light intensity, respectively. Instead, there were extremely significant positive correlations between survival rate and relative humidity and semi-lethal temperature, respectively. Multiple linear regression analysis showed that high light intensity was the key factor for the decrease of survival rate in ‘Georgia Peach’ whereas the survival rate of ‘Petite pearl fairy’ and ‘Tapestry’ in summer mainly depended on the semi-lethal high temperature and extreme high temperature, respectively. References | Related Articles | Metrics

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Characteristics of Tapiscia sinensis Community in Guanshan Nature Reserve, Jiangxi and Its Protection WANG Qian, CHEN Jing-rui, XIONG Yong, LIU Zhong-cheng, LIU Wei-qiu, LIAO Wen-bo 2021, 50(02): 125-132.  DOI: 10.3969/j.issn.1009-7791.2021.02.009 Abstract ( )   PDF (408KB) ( )   The typical Tapiscia sinensis community in Guanshan Nature Reserve of Jiangxi Province was investigated and studied. The results are as follows. There are 104 species belonging to 78 genera and 54 families. The generic areal-types are mainly composed by North Temperate, followed by Pantropic, which demonstrates a typical mid-subtropical montane community. The analysis of the important value of the stumpage species shows that Castanopsis tibetana, T. sinensis, Phoebe sheareri and Alniphyllum fortunei are domiment species of the community. The analysis of diameter class and height class shows that T. sinensis population is in decline stage but basically stable and rather competitive as the result of its dominance at the top level. The Raunkiaer frequency grade of the community is A>B>D>C>E, which is consistent with subtropical montane evergreen broad leaved forest. Simpson diversity index D=0.88, Shannon-Wiener diversity index H=3.65, Pielou evenness EH=0.73. With relatively high community diversity, the community of T. sinensis investigated in this paper is one of the few T. sinensis communities in Guanshan area, which has important scientific research value and tangible value. However, the group of T. sinensis is in declining form, so it is advisable to protect it in situ or ex situ in the future. References | Related Articles | Metrics

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Diversity of Bryophytes and Its Relationship with Environment in Zijin Mountain National Forest Park, HUA Zhuang-zhuang, LU Jian-guo, ZHOU Bei-ning 2021, 50(02): 133-139.  DOI: 10.3969/j.issn.1009-7791.2021.02.010 Abstract ( )   PDF (356KB) ( )   In order to deeply understand the bryophyte resources of Zijin Mountain National Forest Park, a field survey of bryophytes in Nanjing Zijin Mountain was carried out, and canonical correspondence analysis (CCA) was used to study the relationship between the distribution of bryophytes and the environment. The results were as follows. The survey recorded 65 species of bryophytes in 37 genera of 23 families. Brachytheciaceae, Pottiaceae, Thuidiaceae, and Entodontaceae were the dominant families (≥6 species). Entodon, Brachythecium, Plagiomnium, and Bryum were the dominant genera (≥4 species). The bryophyte habitat was mainly soil, with the least trees. The life-forms of bryophytes were dominated by weft and turf, accounting for 46.15% and 35.38%, respectively. The flora was dominated by temperate and East Asian components, showing obvious transitional characteristics. The CCA ranking results showed that the humidity, aspect and elevation were the main environmental factors affecting the distribution of bryophytes. References | Related Articles | Metrics

Plant protection

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Identification of Post-harvest Pathogens of Tomato and Bacteriostasis of Cinnamon Essential Oil on Them CAI Jin-ling, LAN Bo-miao, CHEN Pin-pin, YAO Wen, LIN Tao 2021, 50(02): 140-146.  DOI: 10.3969/j.issn.1009-7791.2021.02.011 Abstract ( )   PDF (448KB) ( )   In order to clarify the main pathogens during the preservation of tomato, rDNA-ITS protocol was applied to identify eight pathogens with different morphology isolated from Solanum lycopersicum var. cerasiforme fruits. The bacteriostatic effect of cinnamon essential oil on these post-harvest pathogens was studied by using direct contact in vitro and inhibition zone. The results were as followed: The eight pathogens could be divided into five categories: Cladosporium, Penicillium, Alternaria, Botrytis and Trichothecium. Cinnamon essential oil had a moderate bacteriostatic effect on these five clusters of pathogens. The inhibitory performances from strong to weak were Trichothecium roseum, Alternaria brassicae, Penicillium expansum, Cladosporium cladosporioides, Botrytis cinerea. In the experiment of sensitivity performances, the most sensitive pathogen was T. roseum, and the least sensitive one was C. cladosporioides. Using GC-MS analysis for cinnamon essential oil composition, 75% of the ingredients had antibacterial effect. The study of bacteriostatic experiment showed that cinnamon essential oil had promisingly bacteriostatic effect and could be used as a natural preservative for tomato after harvesting. References | Related Articles | Metrics

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Investigation and Control Measures of Rhynchophorus ferrugineus in Xiamen, Fujian LI Yan 2021, 50(02): 147-154.  DOI: 10.3969/j.issn.1009-7791.2021.02.012 Abstract ( )   PDF (390KB) ( )   The occurrence, harm characteristics and effective control measures of red palm weevil (Rhynchophorus ferrugineus) in Xiamen Botanical Garden were studied by theoretical analysis, field trapping, laboratory efficacy and actual control effect. Results showed that the generations of red palm weevil in Xiamen overlap seriously, 2 or 3 generations occur a year, each insect state may be as overwintering insect state. The main harm period was from March to November, the peak period of adult trapping was from April to June. The laboratory efficacy of larve was that 45% Acetamiprid-monosultap was superior to 70% Imidacloprid and 1.14% Abamectin-aminomethyl. The laboratory efficacy of adults was that 45% Acetamiprid-monosultap in 1000 times slightly better than 16000 IU·mg-1 Bacillus thuringiensis and 70% Imidacloprid. The laboratory efficacy of adults was that 8000 IU·μL-1 Bacillus thuringiensis in 2000 times was the best, followed by 40 billion spores per gram Beauveria bassiana. By adopting comprehensive control measures, carrying out quarantine inspection and treatment of newly introduced seedlings strictly, strengthen cultivation management to improve the tree potential, reduce plant’s internal consumption, enhance the ability of resistance to diseases and pests, chemical control by means of bagging method , root-irrigation and spraying method, and the affected plants and insect population density can be reduced effectively. References | Related Articles | Metrics

Review

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Formation Mechanism of Blue Flowers in Ornamental Plants LI Han-chun, ZHANG Da-sheng, LIU Qing-qing, LIU Feng-luan, LIU Zhuo-xing, XIONG Lei, AN Xiang-jie, TIAN Dai-ke 2021, 50(02): 155-162.  DOI: 10.3969/j.issn.1009-7791.2021.02.013 Abstract ( )   PDF (303KB) ( )   Blue color is not common in flowers of ornamental plants and it is also a breeding goal for flower breeders. In this paper, the influencing factors related to biosynthesis of anthocyanins, types of anthocyanins, transfer and accumulation of anthocyanins in vacuoles, covalent modification of anthocyanins, inter molecular copigmentation, metal ions and vacuolar pH were discussed. References | Related Articles | Metrics