Subtropical Plant Science

Identification and Expression Analysis of Trihelix Transcription Factor Family in Tobacco

LI Dong-cheng, LI Kui-yin, WEI Xing-qi, DUAN Li-li, MO Ze-jun, LIU Ren-xiang

2022, 51(1): 1-12. DOI: 10.3969/j.issn.1009-7791.2022.01.001

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Tobacco is a kind of temperature-loving economic crop. Screening and utilization of cold-resistant genes plays an important role in the growth and development, yield and quality of tobacco. In this study, bioinformatics analysis was used to identify and analyze the physical and chemical properties, chromosome distribution, chromosome collinearity, motif composition, gene structure, cis-acting elements and expression of Trihelix family genes in tobacco genome data, and the structure and function of tobacco Trihelix family members were preliminarily analyzed. Thirty-two Trihelix genes were identified from common tobacco, which were divided into 5 subfamilies: GT-1, GT-2, GTγ, SH4 and SIP1. Thirty-two NtTH genes had 10 different motifs and different gene structures, but were similar in the same subfamily. There were homologous Trihelix genes in tobacco, tomato, Arabidopsis and rice, and there were collinear genes in the Trihelix family of common tobacco. Thirteen different types of cis elements were identified, which were mainly related to abiotic stress, hormone and growth and development. ABRE and MeJA were the two most cis elements, indicating that the function of NtTH gene was mainly related to abiotic stress and the regulation of growth and development response. Among them, 11 genes, such as tobacco NtTH3 and NtTH16, played an important role in response to low temperature stress. In this study, the genes related to cold resistance of tobacco were found, which provided a theoretical basis for the genetic improvement of cold resistance and stress resistance of tobacco.

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Over-expression of NtMYB4a Gene Enhances Drought Resistance of Tobacco

LI Hong-qiang, LUO Qian, YANG Shuai, XIE Rui-ying, NIE Qiong

2022, 51(1): 13-18. DOI: 10.3969/j.issn.1009-7791.2022.01.002

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In order to explore the ability of transcription factor NtMYB4a to participate in the regulation of tobacco (Nicotiana tabacum) resistance to drought stress, this study used NtMYB4a over-expressed strains (OE), knockout strains (KO) and wild type (WT) as materials, and simulated natural drought stress through pot experiment. Photosynthetic characteristics, root activity, the contents of chlorophyll, proline and malondialdehyde and antioxidant enzyme activities in three strains under drought stress at 0, 2, 4, 6, 8 days and 2 days after re-watering were compared and analyzed. The results showed that net photosynthetic rate (Pn), root activity, chlorophyll content, Pro content, and activities of SOD, POD and CAT in OE strains were significantly higher than those of WT strains, while transpiration rate (Tr), stomatal conductance (Gs) and MDA content were significantly lower than those of WT strains. The Pn, root activity, chlorophyll content, Pro content, activities of SOD, POD and CAT of KO strains were significantly lower than those of WT strains, while Tr, Gs and MDA content of KO strains were significantly higher than those of WT strains. Therefore, the over expression of NtMYB4a should improve the drought resistance of tobacco seedlings by increasing photosynthetic capacity, root activity and antioxidant enzyme capacity, and reducing cell membrane damage.

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Effect of Different Soil Moisture on Root Growth and Architecture of Cinnamomum camphora

QI Jun

2022, 51(1): 19-25. DOI: 10.3969/j.issn.1009-7791.2022.01.003

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The 1-year-old seedlings of Cinnamomum camphora were used as test materials, and the control group (CK), moderate drought treatment (M) and severe drought treatment (S) were set to compare above-ground growth and root configuration of the seedlings among different treatments. The results showed that the accumulation of dry matter, root length, root surface area, root diameter and number of root tips of C. camphora in the moderate and severe drought treatment groups were significantly smaller than those of control group (P<0.05). Drought also significantly increased the root topology index and reduced the fractal dimension and mean branching angle of roots in C. camphora seedlings (P<0.05). The degree of soil moisture and the duration of treatment significantly affected the growth of C. camphora roots and their placement in the soil. Lower soil moisture treatments significantly inhibited root length extension, root surface area expansion and root proliferation, and as soil moisture levels continued to decrease and treatment times increased, the inhibition of C. camphora seedling root growth by water deficit increased and root establishment costs increased while root branching complexity decreased and root systems had to grow and elongate in steeper, deeper directions to improve water uptake. The root system must improve water uptake efficiency by elongating in a steeper and deeper direction. It was recommended that scientific water management strategies should be developed during the maintenance of landscaping projects to meet the needs of soil moisture during the seedling growth of C. camphora.

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Isolation and Identification of an Endophytic Fungi from Azolla and Its Conidial Development Process

HUANG Min-min, CHEN Mei-zhen, CHEN Jian, ZHENG Wei-wen

2022, 51(1): 26-33. DOI: 10.3969/j.issn.1009-7791.2022.01.004

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Water plant Azolla is a symbiotic association between the fern and N-fixing cyanobacterium. This study aimed to isolate and identify its other partner, the endophytic fungi. Through ITS sequencing and electron microscopic observation, the purified isolate was identified to be Aspergillus sydowii which has the potential producing various active substances with economic value. The structural characteristics of conidial development and senescence were explored using transmission electron microscopy. The results revealed that more conidia died via autopygic pathway in the 12 d culture than in 6 d culture, indicating that it was necessary to regulate conidial developmental status by optimizing the fermentation conditions in order to obtain higher product yield in the further research. The autophagic process of the conidia was described and discussed in detail as well.

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Ecology Suitability Regionalization of Polygonatum cyrtonema in Fujian Province

LI Min, WU Ai-qin, LI Xin, SU Ke-bing, YANG Cheng-zi

2022, 51(1): 34-41. DOI: 10.3969/j.issn.1009-7791.2022.01.005

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In order to protect the wild resources of Polygonatum cyrtonema and provide basis for standardized cultivation, the ecological suitability of P. cyrtonema was studied in this paper. 237 samples of P. cyrtonema distribution locations and 55 ecological factors were collected, the maximum entropy (MaxEnt) model was introduced, and the suitable growth areas were analyzed by geographic information system (GIS). The results showed that the main ecological factors affecting the ecological suitability of P. cyrtonema were monthly precipitation in March, annual precipitation, monthly precipitation in June, monthly precipitation in May, monthly precipitation in April, monthly precipitation in February and precipitation in the coldest season. In Fujian province, there is a wide area suitable for P. cyrtonema growth. The prediction results of this study are reliable, which can provide protection for wild resources of P. cyrtonema and provide guidance for scientific planting.

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Determination of Thermal Conductivity and Convective Heat Transfer Coefficient of Leaves of 10 Species of

SUN Yi, ZHANG Yan-jin, FU Hai-ming

2022, 51(1): 42-47. DOI: 10.3969/j.issn.1009-7791.2022.01.006

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In order to study the heat transfer characteristics of plant layer, this paper selected ten species of common landscape plants on the campus, and determined the thermal conductivity of their leaves, convective heat transfer coefficient between leaves and surrounding air, conducts several experimental measurements and analyses, fit the approximate relationship between thermal conductivity and leaf water content, compare the experimentally determined convective heat transfer coefficient with the convective heat transfer coefficient obtained through theoretical calculation of empirical formula, and compare the relative humidity of air before and after contact with leaves. The results showed that there was a cooling and humidifying effect of the leaves; the thermal conductivity of the blade changed less with temperature at 5 to 25 ℃; When the leaf temperature was 20 ℃, the thermal conductivity of the blade decreased with the reduction of leaf water content; the convective heat transfer coefficient of the experimental test was in high agreement with the theoretical calculation.

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Comprehensive Evaluation on the Ornamental Value of 67 Species of Acanthaceae Based on Analytic

YANG Sen, MO Hai-bo, SHI Ji-pu

2022, 51(1): 48-57. DOI: 10.3969/j.issn.1009-7791.2022.01.007

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Based on the present collection and conservation of Acanthaceae plants in Xishuangbanna Tropical Botanical Garden (XTBG), Chinese Academy of Sciences (CAS), we used the analytic hierarchy process (AHP) to construct an evaluation system and comprehensively evaluate the ornamental value of 67 Acanthaceae species with potential for application in gardens. The results showed that among the primary indicators, flower ornamentation properties had the highest weight, indicating that flower ornamentation properties were the most significant ornamental value of the Acanthaceae plants. Some of the secondary indicators, including flower (inflorescence) volume, flower (inflorescence) color vividness, and the length of the flowering period, all had more than 10% influence on the ornamental value and had a higher impact on the evaluation results. While the other secondary indicators, including flower (inflorescence) size, post-flowering ornamentation properties, and leaf coverage, all affected the ornamental value by less than 5% and had a lower impact on the evaluation results. The ornamentation value of the 67 Acanthaceae species was rated and graded according to the weighting and scoring criteria. The evaluation concluded that the ornamental value of 12 Acanthaceae species (Megaskepasma erythrochlamys, Asystasia gangetica, Crossandra infundibuliformis, Ruellia simplex, Thunbergia alata, Pachystachys lutea, Thunbergia mysorensis, Justicia betonica, Strobilanthes hamiltoniana, Pachystachys coccinea, Schaueria calytricha, Thunbergia grandiflora) was high, with a comprehensive evaluation grade Ⅰ and good prospects for application in gardens.

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Morphological and Microscopic Identification of Anoectochilus nandanensis

PENG Meng-chao, ZHENG Cheng-jian, WU Jian-guo, WU Yan-bin, WU Jin-zhong

2022, 51(1): 58-61. DOI: 10.3969/j.issn.1009-7791.2022.01.008

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The pharmacognostical identification method was used to examine the original plant, tissue structure and microscopic characteristics of Anoectochilus nandanensis. Leaves were ovate or ovate elliptic with golden red veins. Non-inverted white flowers had Y-shaped and yellow labellum, which were anteriorly enlarged and 2-lobed. The lobes were narrowly oblong, and its two sides showed the serration or downward angle shape, with 4 to 5 white serration on each side. In the microscopic structure, the cortex in the cross section of root and stem was obvious, with calcium oxalate needle crystal and mucous cells. The epidermal cells on the cross section of the leaves were papilloid in shape. This study provides reference basis for the identification of crude drugs and the exploitation and utilization of the resources.

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Study of Vascular Plant Type Specimens from Fanjing Mountain National Nature Reserve of Guizhou

KUANG Juan, LIU Ting, QUAN Zhi-xin, DUAN Lin-dong, KANG Gong-ping, XIAO Jia-wei

2022, 51(1): 62-66. DOI: 10.3969/j.issn.1009-7791.2022.01.009

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Based on extensive investigation of the related type specimens, 91 vascular plant species (including infraspecies) belonging to 47 families and 73 genera with Fanjing Mountain National Nature Reserve as the model origin were counted. All of them were collected by 31 collectors except one species without collector information, and preserved in 21 research institutions worldwide. Among the species with this area as the model origin, 19 species are named after Fanjing Mountain. Among the original scientific names, 17 were merged, of which 12 changed at the species level and 5 changed at the subspecies level. There are 15 threatened species, including one critically endangered (CR) species, six endangered (EN) species and eight vulnerable (VU) species.

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Two Species of Newly Recorded Seed Plants from Hunan Province

FAN Shi-guang, ZHAO Wan-yi, LIU Zhong-cheng, ZHANG Zhong, LI Xu-jie, Liao Wen-bo

2022, 51(1): 67-69. DOI: 10.3969/j.issn.1009-7791.2022.01.010

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The new records of two species, Tricyrtis viridula Takah. and Rhododendron microcarpum R. L. Liu et L. M. Gao, were reported to Hunan Province of China. Both species were discovered in Luoxiao Mountains. The main recognition features, classification history and floristic geographic implications of these new recorded species were also discussed.

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Three New Records of Angiosperms in Fujian Province

LIN Yi-zhe, FU Hou-hua, CHEN Shuai, CHEN Xin-yan, CHEN Shi-pin

2022, 51(1): 70-72. DOI: 10.3969/j.issn.1009-7791.2022.01.011

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Three species of angiosperms, i.e. Clerodendrum kwangtungense Hand.-Mazz., Symplocos chaoanensis F. G. Wang & H. G. Ye, Phyllanthus tenellus Roxb., were reported as new records in Fujian Province, China. Among them, P. tenellus belongs to newly naturalized species. Certificate specimens were deposited in the Herbarium of College of Forestry, Fujian Agriculture and Forest University (FJFC).

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Research Progress on in vitro Regeneration of Pineapple

SUN Jing-lang, ZHOU Hui-jing, ZHENG Ke-yuan, DAI Xi-ling, ZHU Mu-lan

2022, 51(1): 73-80. DOI: 10.3969/j.issn.1009-7791.2022.01.012

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Pineapple has high edible, medicinal and ornamental value, but there are problems such as low seed germination rate, difficulty in genetic transformation, and lack of gene editing system. Its basic research and large-scale breeding of high-quality seedlings urgently need efficient and reliable in vitro regeneration system. This paper reviewed the research progress in seven aspects: initial explant selection, explant sterilization, basic medium determination, direct adventitious bud formation, indirect adventitious bud formation, somatic embryogenesis, adventitious root induction, and seedling transplanting, in order to provide theoretical and technical reference for pineapple cell engineering breeding, genetic resource improvement and seedling factory production.

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