关键词: 黄独, 脱毒苗, 快繁, 产业化技术

Abstract: In this paper, effects of different factors on factory production technology were studied by using Dioscorea bulbifera virus-free plantlets as test material. The results showed that during factory production of Dioscorea bulbifera virus-free plantlets, white sugar could substitute sucrose, jam bottle could replace triangle tapered flask, being cultured in liquid media was superior to being cultured in solid media, but tap water could not supersede distilled water. In the transplanting process of Dioscorea bulbifera virus-free plantlets, the pore closure rate reached the highest on 5th day, from then on the pores resumed closure function gradually. There were significant differences in fresh weight and diameter of tuber between virus-free plantlets and plantlets with virus, which indicated that the production could be increased after virus elimination.

Key words: Dioscorea bulbifera, virus-free plantlets, rapid propagation, factory production technology