亚热带植物科学 ›› 2012, Vol. 41 ›› Issue (02): 19-22.DOI: 10.3969/j.issn.1009-7791.2012.02.005

• 研究报告 • 上一篇    下一篇

不同浓度6-BA及KT对马铃薯无菌苗诱导的影响

余如刚,杜雪玲,宋运贤   

  1. 淮北师范大学生命科学学院,资源植物学安徽省重点实验室,安徽 淮北 235000
  • 收稿日期:2012-01-19 修回日期:2012-01-19 出版日期:2012-06-10 发布日期:2012-06-10
  • 通讯作者: 余如刚
  • 作者简介:余如刚(1976-),男,宁夏盐池人,硕士,从事植物生理学研究。
  • 基金资助:

    安徽省高等学校省级自然科学研究项目(KJ2009B064Z)、淮北师范大学校青年项目(700295)

Effects of Different Cytokinins on Induction of Potato Sterile Plantlet

YU Ru-gang,DU Xue-ling,SONG Yun-xian   

  1. College of Life Science, Huaibei Normal University, Huaibei 235000, Anhui China
  • Received:2012-01-19 Revised:2012-01-19 Online:2012-06-10 Published:2012-06-10
  • Contact: YU Ru-gang

摘要: 以‘高原7 号’马铃薯茎尖为外植体,用0.1%升汞和75%酒精消毒,经组织培养获得脱毒马铃薯茎段,在增殖培养中,研究不同浓度6-BA 及KT 对马铃薯茎段诱导增殖效果的影响。结果表明:用0.1%升汞对茎尖消毒8 min 效果最好,灭菌率达96.67%。细胞分裂素对马铃薯茎段增殖的促进作用为6-BA>KT,以1.5 mg/L 6-BA 诱导不定芽及其生长势效果最佳。

关键词: 马铃薯, 茎尖培养, 茎段, 细胞分裂素

Abstract: Shoot tips of ‘Highland No.7’ potato are selected as explants and 0.1% Mercuric Chloride and 75% alcohol are employed to disinfect; then through tissue culture, virus-free potato stems are obtained. In the process of enrichment culture, the paper tries to analyze the effect that 6-BA and KT with different consistency have on proliferation-inducing result of potato stems. The result indicates that it is the most effective to use 0.1% Mercuric Chloride to disinfect the shoot tips for 8 minutes, by which the disinfection rate can be up to 96.67%. The better promoting effect of potato stem proliferation made by cytokinin can be achieved when 6-BA > KT; adventitious bud induction and growth potential are the best when the consistency of 6-BA reaches 1.5 mg/L.

Key words: potato, shoot tip culture, stem, cytokinin

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