亚热带植物科学 ›› 2000, Vol. 29 ›› Issue (03): 21-24.

• 研究报告 • 上一篇    下一篇

月光花素对彩叶芋愈伤组织细胞生长和分化的影响

陈德海,陈林娇,辛泽毓,杨盛昌   

  1. 厦门大学 植物基因与基因技术研究所, 福建 厦门 361005
  • 收稿日期:2000-06-16 出版日期:2000-09-10 发布日期:2000-09-10
  • 作者简介:陈德海(1943-),男,福建龙岩人,副教授,从事植物生理学和细胞分子生物学的教学和科研工作。

Effects of calonyctin on growth and differentiation of Caladium bicolor callus

CHEN De-hai, CHEN Lin-jiao, XIN Ze-yu, YANG Sheng-chang   

  1. Institute of Plant Gene and Genetechnique, Xiamen University, Xiamen 361005, China
  • Received:2000-06-16 Online:2000-09-10 Published:2000-09-10

摘要: 在含有NAA0.2mg/L,2.4-D 0.2mg/L的MS基础培养基中添加月光花素培养彩叶芋愈伤组织,结果显示,低浓度的月光花素能促进愈伤组织生长,高浓度则有明显的抑制作用,最适浓度为1.0~2.0mg/L;培养两个月后,添加适宜浓度的月光花素(0.2~2.0mg/L)的培养基能促进体细胞分化和植株再生,而高浓度(10.0mg/L)处理和对照均未能分化出绿苗。表明适宜浓度的月光花素在合适的生长素剂量的协同作用下,对植物愈伤组织细胞的生长和分化有较强的调节作用。

关键词: 月光花素, 彩叶芋, 愈伤组织, 生长和分化, 调节作用

Abstract: Caladium bicolor callus was cultured on the basic MS media containing calonyctin supplemented with 0.2mg/L NAA and 0.2mg/L 2,4-D. The results showed that 0.2~2.0mg/L calonyctin promoted the growth and differentiation of Caladium bicolor callus. After culturing for 2 months, the callus differentiated into plantlets on the medium 0.2~2.0mg/L calonyctin while both control and the callus treated with high concentration of calonyctin (10.0mg/L) did not regenerated plantlets. The results suggested that calonyctin with proper growth regulators has regulatory effects on the growth and differentiation of plant callus.

Key words: calonyctin, Caladium bicolor, callus, growth and differentiation, regulatory effect

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