亚热带植物科学 ›› 2001, Vol. 30 ›› Issue (04): 27-31.

• 研究报告 • 上一篇    下一篇

蓝猪儿组织培养和再生

李玲,黄群声,张铭光,宾金华   

  1. 华南师范大学 生物系, 广东 广州 510631
  • 收稿日期:2001-07-04 出版日期:2001-11-10 发布日期:2001-11-10
  • 作者简介:李玲(1958-),女,湖南桂阳人,教授,博士,从事植物生理学研究工作。
  • 基金资助:

    广东省自然科学基金项目(003062)

Tissue culture and regeneration of Torenia fournieri

LI Ling, HUANG Qun-sheng, ZHANG Ming-guang, BIN Jin-hua   

  1. Department of Biology, South China Normal University, Guangzhou 510631, Guangdong China
  • Received:2001-07-04 Online:2001-11-10 Published:2001-11-10

摘要: 光照有利于蓝猪儿(Torenia fournieri)种子的萌发。上胚轴转接在生芽培养基(MS+0.1 mg/L 4PU+2 mg/L NAA)上,25℃,光暗16/8h培养18d,芽发生,25d不定芽数目达7~8个。叶或茎形成愈伤组织的适宜培养基为MS+0.05 mg/L 2,4-D+0.2 mg/L NAA,愈伤组织再分化形成芽的能力较弱。1/2 MS培养基无论是否添加萘乙酸,蓝猪儿试管苗都能发生不定根,萘乙酸使不定根的数目增多,分布密集。

关键词: 蓝猪儿, 组织培养

Abstract: Illumination could promote the germination of Torenia fournieri seeds. Adventitious buds were produced from epicotyl on MS medium containing 4PU at 0.1mg/L, NAA at 2mg/L with a 16h photoperiod for 18 days at 25℃. 7~8 adventitious buds were obtained after 25 days of culture. A combination of MS added with 2,4-D at 0.05mg/L and NAA at 0.2mg/L gave the best result for leaf and stem to induce calli, but the callus differentiation to buds was difficult. The tube plantlet cultured on 1/2 MS medium could produce roots and NAA was optimum for root number.

Key words: Torenia fournieri, tissue culture

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