关键词: 文心兰, 组织培养, 试管苗

Abstract: The test for passing domestication culture of Oncidium adventitious buds from PLBs showed that taking the modified 1 medium as basic medium supplemented with 100g/L coconut milk for organic additive and no plant-growth regulator were favorable for the PLBs regeneration and could promote the adventitious buds to grow healthy and good uniformity. According to the test of culture medium, organic additive, the amount of activated charcoal, illumination intensity, container and the altitude of the plantlet to fixing into the container, esc, all which relating to the culture elements of PLBs rhizogenesis plantlet strengthening were screened. Results showed that, all plantlets growing from modified 1 medium with 100g/L banana mud, 1g/L activated charcoal, no plant-growth regulator, under the strong light (2 000~3 000 lx), which promoting the growth of plantlets had better performance in each growing index, and they were favorable for the survival of the later domestication. Results showed that also the plants growing in the triangular flask had highest inoculation rate, the lowest contamination rate and better growth index. In the altitude 3 cm of the plantlet to fixing into the container, the efficiency of factory micropropagation of Oncidium was improved.

Key words: Oncidium, tissue culture, plantlet