关键词: 香附子, 块茎, 试管苗, 微型块茎

Abstract: With the method of tissue culture, the tubers of Cyperus rotundus were used as materials to do the research on tube seedlings’ propagation. The results demonstrated that 1/3MS with the matrix of cinder was the ideal medium for adventitious buds growth, and 1/2MS+ZT 0.6 mg/L+NAA 0.1 mg/L was the optimum medium for differentiation cultivation and subculture of growth buds. The ideal medium for rooting and miniature tuber cultivation was 1/2MS+ABT2 6.0 mg/L+ IAA 0.4 mg/L. The transplanting survival rate of tube seedlings after 30 days’ training and 60 days’ training were 96% and 89.7%. The sprout growth rate of tubers was 99.8%. The planting survival rate of tube seedlings and miniature tubers were above 99%. The tube seedlings stable planted maintained all botany characteristics of the wild Cyperus rotundus.

Key words: Cyperus rotundus, tuber, tube seedlings, miniature tuber