关键词: 叶下珠, 组织培养, 快速繁殖

Abstract: Using stem-segment with node of Phyllanthus urinaria as explants, effects of different culture medium and hormones on induction of P. urinaria were analyzed. Using germ-free shoots as material, proliferation, rooting, acclimatization and transplant of P. urinaria were studied. The results showed that the combination of 1/2MS + 6-BA 2.0 mg·L-1 + IBA 0.2 mg·L-1 + agar 7.5 g·L-1 + sucrose 30 g·L-1 + activated carbon 0.5 g·L-1 (pH 5.8—6.0) was the optimal medium for buds induction with induction rate of 100%; the optimum for multiple shoots was the combination of 1/2MS + 6-BA 1.0 mg·L-1 + IBA 0.2 mg·L-1 + agar 7.5 g·L-1 + sucrose 30 g·L-1 (pH 5.8—6.0) and the average multiplication coefficient was 3.8; It was best to rooting under the condition of 1/2MS + IBA 0.5 mg·L-1 + agar 7.5 g·L-1 + sucrose 20 g·L-1 (pH 5.8—6.0) and the rooting rate was 100%. After closing cap 2 d and then opened cap 3 d to practice seedlings and transplant, surviving of tube plantlet rates could reach 86.6%.

Key words: Phyllanthus urinaria, tissue culture, rapid propagation