关键词: 蔓茎堇菜, 柔毛堇菜, 多糖, 抗菌, 抗氧化活性

Abstract: Polysaccharides of Viola diffusa and V. principis were extracted by ethanol precipitation and antimicrobial and antioxidant tests were carried out respectively. The results showed that the extraction rate of V. diffusa and V. principis polysaccharide was 7.0% and 8.3% respectively. The antimicrobial and antioxidant activities of polysaccharides extracted by ethanol precipitation with different multiple volumes were different. Antibacterial experiment indicated that the inhibition zone of V. diffusa polysaccharide to Escherichia coli and Bacillus subtilis reached 8.46 mm and 8.59 mm, while those of V. principis were 9.13 mm. However, neither of the two violets showed inhibitory activity against Aspergillus niger and Saccharomyces cerevisiae. The antioxidant evaluation suggested that antioxidant activity of polysaccharide of V. diffusa was 243.64 U·mL-1, while the antioxidant activity of V. principis was 411.78 U·mL-1. Thus V. principis was significantly stronger than V. diffusa in antimicrobial and antioxidant activities (P<0.01). In general, there was a certain anti-oxidative activity of polysaccharide of V. diffusa and V. principis, therefore, both of them could be used as a resource of medicinal and edible plant for development and utilization.

Key words: Viola diffusa, Viola principis, polysaccharides, antibacterial, antioxidant activity