关键词: 狭叶小金梅草, 花蕾, 增殖, 离体培养

Abstract: The buds of Hypoxis angustifolia were used as material for tissue culturing. The results showed that the optimum culture medium for bud callus induction was MS + 6-BA 0.8 mg·L-1 + NAA 0.05 mg·L-1 + TDZ 0.1 mg·L-1; the culture medium for bud induction was MS + 6-BA 0.5 mg·L-1 + NAA 0.02 mg·L-1 + AC 0.2 g·L-1; the culture medium for propagation was MS + 6-BA 1.0 mg·L-1 + NAA 0.1 mg·L-1 + Huabao-1 1 g·L-1 + AC 0.2 g·L-1. And the subculture circle was 30 d, the multiplication rate was 10-20. The culture medium for root was 1/2 MS + IBA 0.5 mg·L-1 + NAA 0.02 mg·L-1 +AC 0.5 g·L-1. The suitable transplanting medium was surface soil and vermiculite (3:1), the survival rate reached 80%.

Key words: Hypoxis angustifolia, bud, propagation, tissue culture