阔叶百里香鲜、干茎叶精油成分及其抑菌效果

doi:10.3969/j.issn.1009-7791.2021.01.003

亚热带植物科学 ›› 2021, Vol. 50 ›› Issue (01): 15-20.DOI: 10.3969/j.issn.1009-7791.2021.01.003

阔叶百里香鲜、干茎叶精油成分及其抑菌效果

陈品品¹，马晓娟¹，刘靓¹，吴辉煌²，林金秀¹，蓝波妙¹，庄卫东^1*

(1.泉州市农业科学研究所，福建泉州 362212；2.福建省安溪茶厂有限公司，福建安溪 362441)

收稿日期:2020-09-22 修回日期:2020-12-04 出版日期:2021-02-27 发布日期:2021-02-27
通讯作者: 庄卫东
基金资助:
泉州市科技计划项目(2018N054)；福建省科技计划项目(2019N0046)

Component Analysis and Comparison of Antibacterial Activity of Essential Oil from Fresh and Dry Leaves and Stems of Thymus pulegioides

CHEN Pin-pin¹, MA Xiao-juan¹, LIU Liang¹, WU Hui-huang², LIN Jin-xiu¹, LAN Bo-miao¹, ZHUANG Wei-dong^1*

(1.Quanzhou Institute of Agricultural Sciences, Quanzhou 362212, Fujian China; 2.Fujian Anxi Tea Factory Co., Ltd., Anxi 362441, Fujian China)

Received:2020-09-22 Revised:2020-12-04 Online:2021-02-27 Published:2021-02-27
Contact: ZHUANG Wei-dong

摘要/Abstract

摘要： 采用水蒸气蒸馏法和气相色谱-质谱联用技术(GC-MS)分析阔叶百里香(Thymus pulegioides)鲜、干茎叶精油含油率、化学组成及其相对含量的差异，并比较精油对链格孢(Alternaria alternata)、粉红单端孢(Trichothecium roseum)和意大利青霉(Penicillium italicum)的抑制效果。鲜、干茎叶含精油率分别为0.53%、0.37%，分别鉴定出35、40种化合物，其主要化学成分相同，均含有百里香酚(鲜样31.30%，干样26.82%)、麝香草酚甲醚、右旋龙脑和邻伞花烃等，只是相对含量略有差异；在抑菌实验中，百里香精油对链格孢菌的抑制作用最为明显，其次是意大利青霉和粉红单端孢。鲜、干阔叶百里香精油的组分较为接近，相对含量略有差异；阔叶百里香精油对3种真菌具有较好的抑制效果，且鲜样精油的抑菌效果好于干样精油。

关键词: 阔叶百里香, 精油, 成分, 抑菌

Abstract: The essential oil from fresh and dry leaves and stems of Thymus pulegioides was extracted, and their components were detected and identified, and their antibacterial effects were analyzed, so as to provide the basis for the development and application of thyme in this region. Contents of essential oil from fresh and dry thyme were analyzed and compared, by steam distillation extraction and GC-MS. And antibacterial effects of the two essential oils from fresh and dry samples on Alternaria alternate, Trichothecium roseum and Penicillium italicum were compared. The essential oil contents in fresh and dry samples were 0.53% and 0.37%, and total of 35 and 40 compositions were identified, respectively. They had similar main compositions: Thymol(fresh 31.30%, dry 26.82%), 3-methoxy-p-cymene, Borneol and o-Cymene and so on, and these compositions were slightly different in contents. In addition，the inhibitory effect of thyme essential oil on Alternaria alternate was the most significant, the second was Penicillium italicum and Trichothecium roseum. The compositions of essential oils from fresh and dry samples were similar, and the relative contents were slightly different. The essential oil of thyme had a good inhibitory effect on 3 species of fungi, and the antibacterial effect of essential oil of fresh samples was better than that of dry ones.

Key words: Thymus pulegioides, essential oil, composition, antibacterial

中图分类号:

Q946.85

陈品品，马晓娟，刘靓，吴辉煌，林金秀，蓝波妙，庄卫东. 阔叶百里香鲜、干茎叶精油成分及其抑菌效果[J]. 亚热带植物科学, 2021, 50(01): 15-20.

CHEN Pin-pin, MA Xiao-juan, LIU Liang, WU Hui-huang, LIN Jin-xiu, LAN Bo-miao, ZHUANG Wei-dong . Component Analysis and Comparison of Antibacterial Activity of Essential Oil from Fresh and Dry Leaves and Stems of Thymus pulegioides[J]. Subtropical Plant Science, 2021, 50(01): 15-20.

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阔叶百里香鲜、干茎叶精油成分及其抑菌效果

Component Analysis and Comparison of Antibacterial Activity of Essential Oil from Fresh and Dry Leaves and Stems of Thymus pulegioides

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