亚热带植物科学 ›› 2003, Vol. 32 ›› Issue (02): 52-54.

• 研究报告 • 上一篇    下一篇

红掌组培苗继代过程中细菌污染的防治试验

简兴,王米力,石大兴   

  1. 四川农业大学 林学园艺学院, 四川 雅安 625014
  • 收稿日期:2003-01-06 出版日期:2003-06-10 发布日期:2003-06-10
  • 作者简介:简兴(1975-),男,广西桂林人,在读硕士生,从事植物组培研究。

Control of bacteria contamination in sub-culture of Anthurium andraeanum

JIAN Xing, WANG Mi-li, SHI Da-xing   

  1. College of Forestry and Horticulture, Sichuan Agricultural University, Yaan 625014, Sichuan China
  • Received:2003-01-06 Online:2003-06-10 Published:2003-06-10

摘要: 针对红掌组织培养过程中易出现细菌污染的问题,进行多种抗菌素处理及二次升汞灭菌处理对细菌污染的防治比较试验。结果表明,用300mg/L青霉素+180mg/L链霉素溶液注射到培养基表面,能有效控制细菌污染,且对组培苗生长影响最小。

关键词: 红掌, 组织培养, 细菌污染, 抗菌素

Abstract: Anthurium andraeanum is a good ornamental herbaceous plant and easy to be contaminated by bacteria in tissue culture. A comparative experiment treated with antibiotics and HgCl2 to control the bacteria contamination was made. The results showed that 300mg/L penicillin+180mg/L streptomycin could effectively control the contamination of sub-culture for Anthurium andraeanum and it had less effect on the growth of plantlet than any other solutions.

Key words: Anthurium andraeanum, tissue culture, bacteria contamination, antibiotic

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