紫花三叉白芨快繁技术研究

doi:10.3969/j.issn.1009-7791.2022.03.011

亚热带植物科学 ›› 2022, Vol. 51 ›› Issue (3): 227-232.DOI: 10.3969/j.issn.1009-7791.2022.03.011

紫花三叉白芨快繁技术研究

王丽，解兰，李义*

(眉山职业技术学院，四川眉山 620010)

收稿日期:2022-05-27 接受日期:2022-06-15 出版日期:2022-06-30 发布日期:2022-10-17
通讯作者: 李义
基金资助:
眉山职业技术学院科研课题(19KY08)；四川高等职业教育研究中心专项课题(GZY21B31)

Tissue Culture System of Purple Three-pronged Bletilla striata

WANG Li, XIE Lan, LI Yi*

(Meishan Vocational and Technical College, Meishan 620010, Sichuan China)

Received:2022-05-27 Accepted:2022-06-15 Online:2022-06-30 Published:2022-10-17
Contact: LI Yi

摘要/Abstract

摘要： 为建立白芨(Bletilla striata)高效实用的组织培养快繁体系，以紫花三叉白芨成熟未开裂的蒴果、块茎、叶片为外植体，筛选最佳外植体材料，并研究不同生长调节剂浓度培养基对原球茎萌发、丛生芽诱导以及生根影响。结果表明，白芨种子为最佳外植体材料；培养基KC + NAA 0.5 mg·L–1有利于白芨原球茎丛生芽诱导；培养基MS + 6-BA 2.0 mg·L–1 + NAA 0.2 mg·L–1 + 10%椰汁有利于丛生芽增殖及成苗；将2 cm高幼苗转入生根培养基1/2MS + NAA 0.5 mg?L–1中，其生根效果最好，生根率达98.67%。

关键词: 白芨, 组织培养, 原球茎, 萌发率

Abstract: In order to establish an efficient and practical tissue culture and rapid propagation system for Bletilla striata, the mature and undehisced capsule, tuber and leaf were used as explants to screen suitable explants, and the effects of medium with different growth regulator concentrations on the germination of protocorms, the induction of clustered buds and the rooting were studied. The results showed that seed was the best explants material. The medium with KC + NAA 0.5 mg·L–1 was beneficial to cluster bud formation from protocorms. The medium with MS + 2.0 mg·L–1 6-BA + 0.2 mg·L–1 NAA + 10% coconut juice was beneficial to proliferation and seedling formation of multiple shoots. The best rooting medium was 1/2MS + NAA 0.5 mg·L–1 for transfering 2 cm height buds, and the rooting rate was 98.67%.

Key words: Bletilla striata, tissue culture, protocorm, germination rate

中图分类号:

S682.31

王丽，解兰，李义. 紫花三叉白芨快繁技术研究[J]. 亚热带植物科学, 2022, 51(3): 227-232.

WANG Li, XIE Lan, LI Yi. Tissue Culture System of Purple Three-pronged Bletilla striata[J]. Subtropical Plant Science, 2022, 51(3): 227-232.

参考文献

[1] Zhang M, Shao Q, Xu E, Wang Z. Bletilla striata: a review of seedling propagation and cultivation modes[J]. Physiology and Molecular Biology of Plants, 2019, 25(3): 601–609. [2] Wang B, Zhang H, Chen L, Mi Z, Niu J. Extraction, purification, and determination of the gastroprotective activity of glucomannan from Bletilla striata[J]. Carbohydrate Polymers, 2020, 246: 116620. [3] 国家药典委员会. 中华人民共和国药典[M]. 北京: 中国医药科技出版社, 2015: 103. [4] Shi Y, Zhang B, Lu Y, Qian C, Feng Y, Fang L, Ding Z D. Antiviral activity of phenanthrenes from the medicinal plant Bletilla striata against influenza A virus[J]. BMC Complementary and Alternative Medicine, 2017, 17(1): 273. [5] 刘明志, 强娜, 林芳花. 白芨环阿屯烷型三萜的鉴定及抗血管生成活性[J]. 药物生物技术, 2015, 2: 121–124. [6] 杨硕知, 谭著明, 沈宝明, 申爱荣, 谭云. 紫花白芨组织培养技术研究[J]. 湖南林业科技, 2018, 45(3): 10–14. [7] 周涛, 江维克, 魏升华, 陈美兰. 野生白芨的资源调查和利用现状分析[C]//中华中医药学会第九届中药鉴定学术会议论文集. 北京: 中国中医药学会, 2008. [8] 张建霞, 付志惠, 李洪林, 杨波. 白芨胚发育与种子萌发的关系[J]. 亚热带植物科学, 2005, 34(4): 32–35. [9] 王楷, 李玥, 张云峰. 白芨种子的高效萌发及其无性繁殖体系的构建[J]. 云南师范大学学报(自然科学版), 2014, 34(4): 71–76. [10] 刘军凯. 白芨细胞悬浮体系的建立及其次生代谢产物的测定[D]. 昆明: 云南农业大学硕士学位论文, 2012. [11] 高龙梅, 胡振兴, 刘小英, 杨小丽, 李薇, 贾荟芹, 张玲. 白芨组织培养研究进展[J]. 南方农业, 2017, 11(1): 74–76. [12] 马玲, 仇文婷, 王彦军, 康天兰, 栗孟飞. 珍稀中药材白芨组培快繁体系的建立[J]. 中国农学通报, 2020, 19: 80–84. [13] 马彧博, 陈佩. 白芨组织培养方法专利分析[J]. 中国科技信息, 2021, 15: 17–19. [14] 潘学峰, 符式钦, 戴卫端. 青天葵叶片离体培养及植株再生[J]. 海南大学学报(自然科学版), 2001, 19(4): 56–60. [15] 蒋园园, 王兆春, 王凯渊, 谢雪曼, 杨晓红. 一种白芨种子快发壮生的培育方法[P]. 中国: CN107173224A. 2017- 09-19. [16] 李云海, 黑有兰, 陈丽华. 不同浓度6–BA对白芨丛芽诱导的影响[J]. 现代农业科技, 2019, 15: 77–80. [17] 陈灿. 白芨组培快繁技术体系的建立研究[D]. 长沙: 湖南农业大学硕士学位论文, 2016. [2] Vaknin Y, Mishal A. The potential of the tropical “miracle tree” Moringa oleifera and its desert relative Moringa peregrina as edible seed-oil and protein crops under Mediterranean conditions[J]. Scientia Horticulturae, 2017, 225: 431–437. [3] Abd-El-Baky H H, El-Baroty G S. Characterization of Egyptian Moringa peregrine seed oil and its bioactivities[J]. International Journal of Management Sciences and Business Research, 2013, 7: 99–108. [4] Mahmood K T, Mugal T, Haq I U. Moringa oleifera: a natural gift–a review[J]. Journal of Pharmaceutical Sciences and Research, 2010(2): 775–781. [5] Gopalakrishnan L, Doriya K, Kumar D S. Moringa oleifera: A review on nutritive importance and its medicinal application[J]. Food Science and Human Wellness, 2016(5): 49–56. [6] 刘凤霞, 王苗苗, 赵有为, 徐晓云, 潘思轶. 辣木中功能性成分提取及产品开发的研究进展[J]. 食品科学, 2015, 36(19): 282–286. [7] Coppin J P H, Juliani H R, Wu Q L, Simon J E．Variations in polyphenols and lipid soluble vitamins in Moringa oleifera[M]. Processing and Impact on Active Components in Food, Pittsburgh: Academic Press, 2015: 655–663. [8] 虎虓真, 陶宁萍, 许长华. 基于食药价值的辣木籽研究进展[J]. 食品科学, 2018, 39(15): 302–309. [9] Aviara N A, Musa W B, Owolarafe O K, Ogunsina B S, Oluwole F A. Effect of processing conditions on oil point pressure of Moringa oleifera seed[J]. Food Scientists & Technologists, 2015, 52: 4499–4506. [10] Ndubuaku U M, Ndubuaku T C N, Ndubuaku N E. Yield characteristics of Moringa oleifera across different ecologies in Nigeria as an index of its adaptation to climate change[J]. Sustainable Agriculture Research, 2014, 3(1): 95–100. [11] Ojiako E N, Okeke C C. Determination of antioxidant of Moringa oleifera seed oil and its use in the production of a body cream[J]. Asian Journal of Plant Science and Research, 2013(3): 1–4. [12] Ayerza R. Seed characteristics, oil content and fatty acid composition of moringa (Moringa oleifera Lam.) seeds from three arid land locations in Ecuador[J]. Industrial Crops & Products, 2019, 140: 111575. [13] Amaglo N K, Bennett R N, Curto R B L, Rosa E A S, Turco V L, Giuffrida A, Curto A L, Crea F, Timpo G M. Profiling selected phytochemicals and nutrients in different tissues of the multipurpose tree Moringa oleifera L., grown in Ghana[J]. Food Chemistry, 2010, 122: 1047–1054. [14] Wiltshire F M S, Santos A F, Silva L K B, Almeida L C, Freitas L S, Lima A S, Fricks A T, Dariva C, Soares C M F. Influence of seasonality on the physicochemical properties of Moringa oleifera Lam. seed oil and their oleochemical potential[J]. Food Chemistry, 2022, 4: 100068. [15] Mbikay M. Therapeutic potential of Moringa oleifera leaves in chronic hyperglycemia and dyslipidemia: a review[J]. Frontiers in Pharmacology, 2012(3): 1–24. [16] Fakayode O A, Ajav E A. Process optimization of mechanical oil expression from Moringa (Moringa oleifera) seeds[J]. Industrial Crops and Products, 2016, 90: 142–151. [17] Guzman-Albores J M, Velazquezb E B, Leon-Rodríguez A D, Calva-Cruz O D J, Ruiz-Valdiviezo V W. Comparison of Moringa oleifera oils extracted with supercritical fluids and hexane and characterization of seed storage proteins in defatted flour[J]. Food Bioscience, 2021, 40: 100830. [18] Haile M, Duguma H T, Chameno G, Kuyu C G. Effects of location and extraction solvent on physical chemical properties of Moringa stenopetala seed oil[J]. Heliyon, 2019, 5(11): e02781. [19] Yusoff M M, Gordon M H, Niranjan K. Aqueous enzyme assisted oil extraction from oil seeds and emulsion de-emulsifying methods: A review[J]. Trends in Food Science & Technology, 2015, 41: 60–82. [20] Yusoff M M, Gordon M H, Ezeh O, Niranjan K. Aqueous enzymatic extraction of Moringa oleifera oil[J]. Food Chemistry, 2016, 211: 400–408. [21] Zhong J F, Wang Y H, Yang R, Liu X, Yang Q Q, Qin X L. The application of ultrasound and microwave to increase oil extraction from Moringa oleifera seeds[J]. Industrial Crops & Products, 2018, 120: 1–10. [22] Sánchez-Machado D I, López-Cervantes J, Nú?ez-Gastélum J A, Mora-López G S, López-Hernández J, Paseiro-Losada P. Effect of the refining process on Moringa oleifera seed oil quality[J]. Food Chemistry, 2015, 187: 53–57. [23] 赵翊君, 赵谋明, 陈森林, 陶红, 赵强忠. 辣木籽酶解产物的蛋白回收率及DPPH?清除能力的研究[J]. 现代食品科技, 2017, 33(12): 77–83. [24] 赵谋明, 李巧琳, 林恋竹, 杨延清. 辣木叶提取物的制备及抗氧化活性[J]. 食品科学, 2018, 39(21): 25–30. [25] Baptista L T A, Silva M O, Gomes R G, Bergamasco R, Vieira M F, Vieira A M S. Protein fractionation of seeds of Moringa oleifera Lam. and its application in superficial water treatment[J]. Separation and Purification Technology, 2017, 180: 114–124. [26] 孙丹, 管俊岭, 许玫, 李涛, 陈文品. 辣木的有效成分保健功能和开发利用研究进展[J]. 热带农业科学, 2016, 36(3): 28–33. [27] Ghebremichael K A, Gunaratna K R, Henriksson H, Brumer H, Dalhammar G. A simple purification and activity assay of the coagulant protein from Moringa oleifera seed[J], Water Research, 2005, 39: 2338–2344. [28] Dezfooli S M, Uversky V N, Saleem M, Baharudin F S, Hitam H M S, Bachmann R T. A simplified method for the purification of an intrinsically disordered coagulant protein from defatted Moringa oleifera seeds[J]. Process Biochemistry, 2016, 51: 1085–1091. [29] Bassogog C B B, Nyobe C E, Ngui S P, Minka S R, Mune M A M. Effect of heat treatment on the structure, functional properties and composition of Moringa oleifera seed proteins[J]. Food Chemistry, 2022, 384: 132546. [30] Bennett R N, Mellon F A, Foidl N, Pratt J H, Dupont M S, Perkins L, Kroon P A. Profiling glucosinolate and phenolics in vegetative and reproductive tissues of the multi-purpose trees Moringa oleifera L. (Horseradish Tree) and Moringa stenopetala L.[J]. Journal of Agricultural and Food Chemistry, 2003, 51: 3546–3553. [31] Maldini M, Maksoud S A, Natella F, Montoro P, Petretto G L, Foddai M, Nicola G R D, Chessa M, Pintore G. Moringa oleifera: study of phenolics and glucosinolates by mass spectrometry[J]. Journal of Mass Spectrometry, 2014, 49: 900–910. [32] F?rster N, Ulrichs C, Schreiner M, Müller C T, Mewis I. Development of a reliable extraction and quantification method for glucosinolate in Moringa oleifera[J]. Food Chemistry, 2015, 166: 456–464. [33] Brunelli D, Tavecchio M, Falcioni C, Frapolli R, Erba E, Lori R, Rollin P, Barillari J, Manzotti C, Morazzoni P. The isothiocyanate produced from glucomoringin inhibits NF-kB and reduces myeloma growth in nude mice in vivo[J]. Biochemical Pharmacology, 2010, 79: 1141–1148. [34] Waterman C, Cheng D M, Rojas-Silva P, Poulev A, Dreifus J, Lila M A, Raskin L. Stable, water extractable isothiocyanates from Moringa oleifera leaves attenuate inflammation in vitro[J]. Phytochemistry, 2014, 103: 114–122. [35] Jaja-Chimedza A, Zhang L, Wolff K, Graf B L, Kuhn P, Moskal K, Carmouche R, Newman S, Salbaum J M, Raskin L. A dietary isothiocyanate–enriched Moringa (Moringa oleifera) seed extract improves glucose tolerance in a high–fat–diet mouse model and modulates the gut microbiome[J]. Journal of Functional Foods, 2018, 47: 376–385. [36] 杨扬, 贺水莲, 唐明霆, 戴吴兰, 史崇颖, 田盛军. 从辣木籽中制备 4-α-L-鼠李糖基-异硫氰酸苄酯的工艺优化研究[J]. 云南农业大学学报(自然科学), 2019, 34(2): 303–308. [37] Ojiako E N, Okeke C C. Determination of antioxidant of Moringa oleifera seed oil and its use in the production of a body cream[J]. Asian Journal of Plant Science and Research, 2013(3): 1–4. [38] Sánchez-Machado D I, López-Cervantes J, Nú?ez-Gastélum J A, Mora-LópezG S, López-Hernández J, Paseiro-Losada P. Effect of the refining process on Moringa oleifera seed oil quality[J]. Food Chemistry, 2015, 187: 53–57. [39] 殷春雁, 刘自单, 马瑞菊, 刘超. 辣木果胚油的体外抗氧化活性研究[J]. 中国新技术新产品, 2019(5): 22–23. [40] Lalas S, Gortzi O, Athanasiandis V, Tsaknis J, Chinon I. Determination of antimicrobial activity and resistance to oxidation of Moringa peregrine seed oil[J]. Molecules, 2012, 17(3): 2330–2334. [41] Lin L, Gu Y, Cui H. Moringa oil/chitosan nanoparticles embedded gelatin nanofibers for food packaging against Listeria monocytogenes and Staphylococcus aureus on cheese[J]. Food Packaging and Shelf Life, 2019, 19: 86–93. [42] Cui H Y, Li H, Li C Z, Abdel–Samie M A, Lin L. Inhibition effect of Moringa oil on the cheese preservation and its impact on the viability, virulence and genes expression of Listeria monocytogenes[J]. LWT-Food Science and Technology, 2020, 134: 110163. [43] Vieira G H F, Mourao J A, Angelo A M, Costa R A, Silva R H, Vieira S F. Antibacterial effect (in vitro) of Moringa oleifera and Annona muricata against Gram positive and Gram negative bacteria[J]. Revista Do Instituto De Medicina Tropical De S?o Paulo, 2010, 52(3): 129–132. [44] Moyo B, Masika P J, Julius, Muchenje V. Antimicrobial activities of Moringa oleifera Lam leaf extracts[J]. African Journal of Biotechnology, 2012, 11(11): 2797–2802. [45] Freire J , Vasconcelos I M, Moreno F, Batista A, Lobo M, Pereira M L, Lima J, Almeida R, Sousa A, Monteiro-Moreira A, Oliveira J, Grangeiro T. Mo-CBP3, an antifungal chitin-binding protein from Moringa oleifera Seeds, is a member of the 2S albumin family[J]. Plos One, 2015, 10(3): e0119871. [46] Neto J, Pereira M , Oliveira J, Rocha-Bezerra L, Lopes T, Costa H, Sousa D, Rocha B, Grangeiro T, Freire J, Monteiro-Moreira A C, Lobo M, Brilhante R, Vasconcelos I. A chitin-binding protein purified from Moringa oleifera seeds presents anticandidal activity by increasing cell membrane permeability and reactive oxygen species production[J]. Front Microbiology, 2017, 8: 980. [47] Vunain E, Masoamphambe E F, Mpeketula P M G, Monjerezi M, Etale A. Evaluation of coagulating efficiency and water borne pathogens reduction capacity of Moringa oleifera seed powder for treatment of domestic wastewater from Zomba, Malawi[J]. Journal of Environmental Chemical Engineering, 2019, 7: 103–118. [48] Shamlan G, Al-Nouri D M, Alathbah A A, Arzoo S, Habibullahb M M. Antiarthritic, anti-inflammatory activity of Moringa peregrina seed oil and leaves in Freund’s complete adjuvant-induced arthritis in rats[J]. Journal of King Saud University Science, 2021, 33(3): 101350. [49] Ventura A C S S B, Paula T, Gon?alves J P, Soley B S, Cretella A B M, Otuki M F, Cabrini D A. The oil from Moringa oleifera seeds accelerates chronic skin wound healing[J]. Phytomedicine Plus, 2021, 1(3): 100099. [50] Galuppo M, Giacoppo S, Nicola G R D, Iori R, Navarra M, Lombardo G E, Bramanti P, Mazzon E. Antiinflammatory activity of glucomoringin isothiocyanate in a mouse model of experimental autoimmune encephalomyelitis[J]. Fitoterapia, 2014, 95: 160–174. [51] Giacoppo S, Galuppo M, Nicola G R D, Iori R, Bramanti P, Mazzon E. 4(α-L-Rhamnosyloxy)-benzyl isothiocyanate, a bioactive phytochemical that attenuates secondary damage in an experimental model of spinal cord injury[J]. Bioorganic & Medicinal Chemistry, 2015, 23: 80–88. [52] Jaja-Chimedza A, Graf B L, Simmler C, Kim Y, Raskin I. Biochemical characterization and antiinflammatory properties of an isothiocyanate enriched Moringa (Moringa oleifera) seed extract[J]. Plos One, 2017(8): 1–21. [53] Santos A O, Val D R, Silveira F D, Gomes F I F, Freitas H C, Almeida D K C, Silva I I C, Barbosa F G, Mafezoli J, Silva M R, Brito G A C, Clemente-Napimoga J T, Pinto V P T P T, Filho G C, Bezerra M M, Chaves H V. Antinociceptive, anti–inflammatory and toxicological evaluation of semisynthetic molecules obtained from a benzyl-isothiocyanate isolated from Moringa oleifera Lam. in a temporomandibular joint inflammatory hypernociception model in rats[J]. Biomedicine & Pharmacotherapy, 2018, 98: 609–618. [54] Brunelli D, Tavecchio M, Falcioni C, Frapolli R, Erba E, Iori R, Rollin P, Barillari J, Manzotti C, Morazzoni P, Incalci M. The isothiocyanate produced from glucomoringin inhibits NF-kB and reduces myeloma growth in nude mice in vivo[J]. Biochemical Pharmacology, 2010, 79: 1141–1148. [55] Rajan T S, Nicola G R D, Iori R, Rollin P, Bramanti P, Mazzon E. Anticancer activity of glucomoringin isothiocyanate in human malignant astrocytoma cells[J]. Fitoterapia, 2016, 110: 1–7. [56] Tiloke C, Phulukdaree A, Anand K, Gengan R M, Chuturgoon A A. Moringa oleifera gold nanoparticles modulate oncogenes, tumor suppressor genes, and caspase-9 splice variants in A549 cells[J]. Journal of cellular biochemistry, 2016, 9999: 1–13. [57] Cuellar-Núnez M L, Loarca-Pina G, Berhow M, Mejia E G. Glucosinolate-rich hydrolyzed extract from Moringa oleifera leaves decreased the production of TNF-α and IL-1β cytokines and induced ROS and apoptosis in human colon cancer cells[J]. Journal of Functional Foods, 2020. https: //doi.org/10.1016/j.jff. 2020.104270. [58] Anwar F, EHussain A I, Iqbal S, Bhanger M I. Enhancement of the oxidative stability of some vegetable oils by blending with Moringa oleifera oil[J]. Food Chemistry, 2007, 103(4): 1181–1191. [59] 段琼芬, 马李一, 王有琼, 张重权, 赵虹. 辣木籽油食用安全性毒理学评价[J]. 中国油脂, 2014, 39(2): 48–52. [60] 王鹏, 陈婷, 孙加节, 习欠云, 张永亮. 辣木在动物饲料中应用的研究进展[J]. 动物营养学报, 2018, 30(7) : 2488–2495. [61] Cardines P H F, Baptista A T A, Gomes R G, Rosangela B, Vieira A M S. Moringa oleifera seed extracts as promising natural thickening agents for food industry: Study of the thickening action in yogurt production[J]. LWT, 2018, 97: 39–44. [62] Shi Y N, Prabakusuma A S, Zhao Q, Wang X, Huang A. Proteomic analysis of Moringa oleifera Lam. leaf extract provides insights into milk-clotting proteases[J]. LWT-Food Science and Technology, 2019, 109: 289–295. [63] Kholif A E, Gouda G A, Morsy T A, Salem A Z M, Lopez S, Kholif A M. Moringa oleifera, leaf meal as a protein source in lactating goat’s diets: feed intake, digestibility, ruminal fermentation, milk yield and composition, and its fatty acids profile[J]. Small ruminant research, 2015, 129: 129–137. [64] Sun B, Zhang Y, Ding M, Xi Q, Liu G, Li Y, Liu D, Chen X. Effects of Moringa oleifera leaves as a substitute for alfalfa meal on nutrient digestibility, growth performance, carcass trait, meat quality, antioxidant capacity and biochemical parameters of rabbits[J]. Journal of Animal Physiology & Animal Nutrition, 2017, 102(1): 194–203. [65] Broin M, Santaella C, Cuine S, Kokou K, Peltier G, Joet T. Flocculant activity of a recombinant protein from Moringa oleifera Lam. seed[J]. Applied Microbiology and Biotechnology, 2002, 60: 114–119. [66] Bhuptawat H, Folkard G K, Chaudhari S. Innovative physico-chemical treatment of wastewater incorporating Moringa oleifera seed coagulant[J]. Journal of Hazardous Materials, 2007, 142: 477–482. [67] Moulin M, Mossou E, Sign L, Kieffer-Jaquinod S, Rennie A R. Towards a molecular understanding of the water purification properties of Moringa seed proteins[J]. Journal of Colloid and Interface Science, 2019, 554: 296–304. [68] Rashid U, Anwar F, Moser B R, Knothe G. Moringa oleifera oil: A possible source of biodiesel[J]. Bioresource Technology, 2008, 99: 8175–8179. [69] Fernandes D, Sousa R, Oliveira A, Morais S, Richter E, Munoz R. Moringa oleifera: a potential source for production of biodiesel and antioxidant additives[J]. Fuel, 2015, 146: 75–80. [70] 黄丽娜, 程世敏, 赵增贤, 魏守兴, 谢子四. 我国辣木产业发展的现状与前景[J]. 贵州农业科学, 2016, 44(7): 104–107. [2] 李欣, 孙美莲, 刘小芬, 叶须钦, 吴爱琴, 安昌, 杨成梓. 福建被子植物分布新记录Ⅺ[J]. 亚热带植物科学, 2021, 50(1): 61–64. [3] 朱艺耀, 林玉麟, 马良, 杨成梓. 福建省被子植物新记录属种[J]. 亚热带植物科学, 2021, 50(1): 69–71. [4] 叶宝鉴, 陈新艳, 陈永滨, 马良, 陈世品. 福建南靖3种省级被子植物新记录[J]. 亚热带农业研究, 2020, 16(2): 106–109. [5] 胡喻华. 叶下珠属植物新资料[J]. 华南农业大学学报, 2006(2): 121–122. [6] 王发国, 叶华谷. 中国山矾属一新种[J]. 热带亚热带植物学报, 2002(1): 35–36. [7] 中国科学院中国植物志编辑委员会. 中国植物志(第65卷第1册)[M]. 北京: 科学出版社, 1982. [8] 中国科学院中国植物志编辑委员会. 中国植物志(第40卷)[M]. 北京: 科学出版社, 1994. [9] 编写组福建省科学技术委员会福建植物志. 福建植物志(第1～6卷)[M]. 福州: 福建科学技术出版社, 1995. [10] 彭绍云. 福建新记录野生植物——总状山矾[J]. 亚热带植物科学, 2013, 42(2): 181. [11] 王清隆, 王茂媛, 杨虎彪, 晏小霞, 王祝年. 海南被子植物分布新资料(Ⅲ)[J]. 热带作物学报, 2020, 41(7): 1484–1489. [12] 曾宪锋, 邱贺媛. 福建省3种新记录归化植物[J]. 福建林业科技, 2013, 40(2): 110–111. [13] 姜秋怡, 侯学良, 王文卿, 张宜辉. 福建植物新资料(一)[J]. 亚热带植物科学, 2013, 42(2): 174–176. 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