关键词: 菰腺忍冬；快繁体系；组织培养；总黄酮；绿原酸

Abstract: Lonicera hypoglauca is a traditional Chinese medicinal plant. In this study, the tender young leaves of L. hypoglauca were used for the first time as the explants to establish a rapid in vitro propagation and regeneration system. The results revealed that the optimal time for disinfection of the explants was 8 min and the optimal medium for callus induction was MS + 2,4-D 4.0 mg·L–1 + sucrose 30 g·L–1, with an average callus induction rate of 86.67%. The optimal medium to induce differentiation of callus to bud was MS + 6-BA 1.0 mg·L–1 + NAA 0.10 mg·L–1 + sucrose 30 g·L–1, with an average germination rate of 83.33%. The optimal medium to induce multiplication was MS + 6-BA 1.5 mg·L–1 + NAA 0.05 mg·L–1 + sucrose 30 g·L–1, with a multiplication coefficient of 5.42. The optimal medium for root induction was 1/2 MS + NAA 0.15 mg·L–1 + activated carbon 0.3 g·L–1 + sucrose 15 g·L–1, with an average rooting rate of 91.11%. The survival rate of tissue-cultured seedlings in nutrient soil cultivation medium was as high as 100%. The total flavonoid content and chlorogenic acid content in the explant, callus tissue and regenerated plant were 1.83%, 2.27%, 1.33% and 2.77%, 1.83%, 1.74% respectively. This study provides novel insights into the rapid propagation and mass production of L. hypoglauca seedlings at an industrial scale and that it exhibits important application value and future prospects.

Key words: Lonicera hypoglauca; rapid propagation system; tissue culture; total flavonoids; chlorogenic acid