丛枝菌根真菌、磷和生长素对枳侧根形成的调控效应

doi:10.3969/j.issn.1009-7791.2016.03.004

›› 2016, Vol. 45 ›› Issue (03): 216-220.DOI: 10.3969/j.issn.1009-7791.2016.03.004

• 植物生理生化与分子生物学 • 上一篇下一篇

丛枝菌根真菌、磷和生长素对枳侧根形成的调控效应

许朋阳,陈伟立,朱红惠,李娟,陈杰忠,姚青

(1.华南农业大学园艺学院，广东广州 510642；2.广东省微生物研究所，广东广州 510070；3.仲恺农业工程学院，广东广州 510225)

收稿日期:2016-04-29 修回日期:2016-10-12 出版日期:2016-09-10 发布日期:2016-09-10
通讯作者: 姚青
作者简介:许朋阳，硕士研究生，从事果树生理与生态研究。
基金资助:
国家自然科学基金项目(31270448)；广东省高等学校人才引进专项(粤财教[2013]246号)

Regulative Effects of Arbuscular Mycorrhizal Fungus, P and Auxin on the Lateral Root Formation of Poncirus trifoliata

XU Peng-yang,CHEN Wei-li,ZHU Hong-hui,LI Juan,CHEN Jie-zhong,YAO Qing

(1.College of Horticulture, South China Agricultural University, Guangzhou 510642, Guangdong China；2.Guangdong Institute of Microbiology, Guangzhou 510070, Guangdong China; 3.Zhongkai University of Agriculture and Engineering, Guangzhou 510225, Guangdong China)

Received:2016-04-29 Revised:2016-10-12 Online:2016-09-10 Published:2016-09-10
Contact: YAO Qing

摘要/Abstract

摘要： 为探讨丛枝菌根真菌(AMF)、磷水平和生长素对植物侧根形成的影响，在两种磷水平下接种AMF(Rhizophagus irregularis BGC JX04B)，施用IBA、生长素运输抑制剂(TIBA)，观察AMF、磷水平和生长素对枳Poncirus trifoliata幼苗侧根形成的调控效应。结果表明，AMF对植株生物量及各级侧根数量无显著影响，但显著降低一级侧根长度；磷水平对植株生物量、侧根数量及长度无显著影响；TIBA显著降低植株生物量、侧根数量和侧根长度，而IBA对各项指标无显著影响。AMF和生长素对主根长度的影响存在显著互作；AMF、磷水平和生长素对二级和三级侧根数量的影响存在显著互作。因此，AMF对枳侧根形成的调控可能涉及生长素信号途径，而生长素运输是枳侧根形成的关键因素。

关键词: 丛枝菌根真菌, 磷水平, 生长素, 侧根形成, 枳

Abstract: To explore the influence of arbuscular mycorrhizal fungus (AMF), P level and auxin on plant lateral root (LR) formation, AMF strain (Rhizophagus irregularis BGC JX04B) was inoculated to Poncirus trifoliata seedlings at two P levels, which was applied with IBA, TIBA and water as control, and the regulative effects of AMF, P level and auxin on LR formation of trifoliate orange were investigated. The results showed that AMF did not significantly affect the plant biomass and LR number, but significantly decreased the 1st order LR length. P level did not significantly affect the plant biomass, LR number and length. TIBA significantly decreased the plant biomass, LR number and length, while IBA did not significantly influence these parameters. AMF and auxin showed significant interaction on the primary root length, and AMF, P level and auxin showed significant interaction on the 2nd and 3rd order LR number. These data suggested that auxin signaling pathway could be involved in the regulation of AMF and P level on LR formation, and the auxin transportation should be the key factor in LR formation of trifoliate orange.

Key words: AMF, P level, auxin, lateral root formation, Poncirus trifoliata

中图分类号:

Q945.45

许朋阳,陈伟立,朱红惠,李娟,陈杰忠,姚青. 丛枝菌根真菌、磷和生长素对枳侧根形成的调控效应[J]. , 2016, 45(03): 216-220.

XU Peng-yang,CHEN Wei-li,ZHU Hong-hui,LI Juan,CHEN Jie-zhong,YAO Qing . Regulative Effects of Arbuscular Mycorrhizal Fungus, P and Auxin on the Lateral Root Formation of Poncirus trifoliata[J]. , 2016, 45(03): 216-220.

参考文献

[1] Smith S E, Read D J. Mycorrhizal Symbiosis[M]. New York: Academic Press, 2010.
[2] Nouri E, Breuillin-Sessoms F, Feller U, Reinhardt D. Phosphorus and nitrogen regulate arbuscular mycorrhizal symbiosis in Petunia hybrida[J]. PloS One, 2014,9(3): e90841.
[3] Yao Q, Wang L R, Zhu H H, Chen J Z. Effect of arbuscular mycorrhizal fungal inoculation on root system architecture of trifoliate orange (Poncirus trifoliata L. Raf.) seedlings[J]. Scientia Horticulturae, 2009,121(4): 458—461.
[4] Nibau C, Gibbs D J, Coates J C. Branching out in new directions: the control of root architecture by lateral root formation[J]. New Phytologist, 2008,179(3): 595—614.
[5] Gruber V, Zahaf O, Diet A, de Zélicourt A, de Lorenzo L, Crespi M. Impact of the environment on root architecture in dicotyledoneous plants[M]// de Oliveira A C, Varshney R K. Root Genomics. Berlin: Springer Berlin Heidelberg, 2011: 113—132.
[6] Li Z, Xu C, Li K, Yan S, Qu X, Zhang J. Phosphate starvation of maize inhibits lateral root formation and alters gene expression in the lateral root primordium zone[J]. BMC Plant Biology, 2012,12(1): 89.
[7] Péret B, Desnos T, Jost R, Jost R, Kanno S, Berkowitz O, Nussaume L . Root architecture responses: in search of phosphate[J]. Plant Physiology, 2014,166(4): 1713—1723.
[8] Lavenus J, Goh T, Roberts I, Guyomarc S, Lucas M, De Smet I, Laplaze L. Lateral root development in Arabidopsis: fifty shades of auxin[J]. Trends in Plant Science, 2013,18(8): 450—458.
[9] Fukaki H, Tasaka M. Hormone interactions during lateral root formation[J]. Plant Molecular Biology, 2009,69(4): 437—449.
[10] Dinh P T Y, Roldan M, Leung S, McManus M T. Regulation of root growth by auxin and ethylene is influenced by phosphate supply in white clover (Trifolium repens L.)[J]. Plant Growth Regulation, 2012,66(2): 179—190.
[11] 庄伊美. 柑桔营养与施肥[J]. 福建果树, 1992(4): 32—37.
[12] Giovannetti M, Mosse B. An evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection in roots[J]. New Phytologist, 1980,84(3): 489—500.
[13] Dubrovsky J G, Sauer M, Napsucialy-Mendivil S, Ivanchenko M G, Friml J, Shishkova S, BenkováE. Auxin acts as a local morphogenetic trigger to specify lateral root founder cells[J]. Proceedings of the National Academy of Sciences, 2008,105(25): 8790—8794.
[14] Teale W D, Paponov I A, Palme K. Auxin in action: signaling, transport and the control of plant growth and development[J]. Nature Reviews Molecular Cell Biology, 2006,7(11): 847—859.
[15] Mayzlish-Gati E, De-Cuyper C, Goormachtig S, Beeckman T, Vuylsteke M, Brewer P B, Wininger S. Strigolactones are involved in root response to low phosphate conditions in Arabidopsis[J]. Plant Physiology, 2012,160(3): 1329—1341.
[16] De Dorlodot S, Forster B, Pagès L, Price A, Tuberosa R., Draye X. Root system architecture: opportunities and constraints for genetic improvement of crops[J]. Trends in Plant Science, 2007,12(10): 474—481.
[17] 邹英宁,吴强盛,李艳,黄咏明. 丛枝菌根真菌对枳根系形态和蔗糖、葡萄糖含量的影响[J]. 应用生态学报, 2014,25(4): 1125—1129.
[18] 牛琳琳,高崇,曾明,李伟,雷世梅,伍加勇. 不同 pH 值下接种AM 真菌对枳橙苗生长及光合作用的影响[J]. 中国南方果树, 2012,41(5): 13—16.
[19] Oláh B, Brière C, Bécard G, Dénarié J, Gough C. Nod factors and a diffusible factor from arbuscular mycorrhizal fungi stimulate lateral root formation in Medicago truncatula via the DMI1/DMI2 signalling pathway[J]. Plant Journal, 2005,44(2): 195—207.
[20] Maillet F, Poinsot V, André O, Puech-Pagès V, Haouy A, Gueunier M, Martinez E A. Fungal lipochitooligosaccharide symbiotic signals in arbuscular mycorrhiza[J]. Nature, 2011,469: 58—63.
[21] Arenas-Huertero F, Arroyo A, Zhou L, Sheen J, León P. Analysis of Arabidopsis glucose insensitive mutants, gin5 and gin6, reveals a central role of the plant hormone ABA in the regulation of plant vegetative development by sugar[J]. Genes & Development, 2000,14(16): 2085—2096.
[22] Nacry P, Canivenc G, Muller B, Azmi A, Van Onckelen H, Rossignol M, Doumas P. A role for auxin redistribution in the responses of the root system architecture to phosphate starvation in Arabidopsis[J]. Plant Physiology, 2005,138(4): 2061—2074.
[23] Fusconi A. Regulation of root morphogenesis in arbuscular mycorrhizae, what role do fungal exudates, phosphate, sugars and hormones play in lateral root formation?[J]. Annals of Botany, 2014,113(1): 19—33.
[24] Kaldorf M, Ludwig-Müller J. AM fungi might affect the root morphology of maize by increasing indole-3-butyric acid biosynthesis[J]. Physiologia Plantarum, 2000,109(1): 58—67.

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丛枝菌根真菌、磷和生长素对枳侧根形成的调控效应

Regulative Effects of Arbuscular Mycorrhizal Fungus, P and Auxin on the Lateral Root Formation of Poncirus trifoliata

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