关键词: 枳, 酸橙, 胚轴, 子叶, 离体培养, 不定芽

Abstract: Epicotyl,hypocotyl and cotyledon of trifoliate orange and sour orange tubed-seedlings were taken as materials in the experiment and cut into several segments, and cultured in vitro. The results showed that the highest percentage adventitious buds formation occured on MS medium added with 10^-4 or 5×10^-5 mol BA, the total number of mean adventitious buds per trifoliate orange explant was up to 41 and 33.5,respectively;the most adventitious buds were formed on the first epicotyl and hypocotyl. Fewer produced aventitious tuds on MS medium supplemented with KT in all treatments.Percentage rooting was the highest on MS medium containing 10^-5 mol NAA. 15-20 days after transferred to MS medium added with 5×10^-6 or 10^-6 mol NAA, the adventitious buds of trifoliate orange rooted and plantlets produced. The number of adventitious buds formed in sour orange was significantly less than that in trifoliate orange in the same treatments.

Key words: Trifoliate orange, Sour orange, hypocotyl, cotyledon, In vitro, Adventitious buds