Abstract: The optimum approach for producing the ORSV-free and CyMV-free Cymbidium hybridum by using three virus deprivation methods was discussed in this research, which were ribavirin treatments combining with shoot tips culture or seedlings and shoot tips came from seedlings had been treated by ribavirin for three months. The results showed that the ribavirin added to the medium treat shoot tips for two weeks was not effective against CyMV and ORSV. Shoot tips came from seedling which were treated by ribavirin was the best method, and the method of ribavirin treatment seedling for three months came second. Ribavirin was more effective against CyMV than ORSV in Cymbidium hybridum.

Key words: Cymbidium hybridum, shoot tips, ribavirin, virus eradication

摘要： 在大花蕙兰茎尖培养中结合病毒唑处理，探讨对建兰花叶病毒(CyMV)和齿兰环斑病毒(ORSV)的脱除效果。结果表明，病毒唑添加到培养基中，处理茎尖2周，脱毒效果低于经病毒唑处理3个月后的幼苗，经病毒唑处理幼苗再结合茎尖培养脱毒效果最好。病毒唑处理对大花蕙兰CyMV的脱除效果优于对ORSV的脱除效果。

关键词: 大花蕙兰, 茎尖, 病毒唑, 脱毒